Ppp2r3a (BC027415) Mouse Tagged ORF Clone

CAT#: MR208433

Ppp2r3a (Myc-DDK-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha (cDNA clone MGC:36855 IMAGE:4235453)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp2r3a
• Synonyms: PR59
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208433 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCTGACGCCGCGGCTACAGCTGAAGGTGGACGAGCTCTTCCGGCGCTGGCTCGGCGATCCTC
  ACACGCAGCGCGCGCTCAGCCACGCCCTCCGCCGCATCCGGGACCCAGGGACGACCTCTGACCCCGCCGC
  CGCCGCGACCCCCGATGCGGACCCCGGAAACACGACCTCCGACCCTAACCCCGCCTCCCGACCGCTGCCG
  CGGCCCGCGCTGAGAACGACGGGACCCAGGACGGTCCATGCCCACAGGAAGGAGGAGCCCCCGCCCCAGT
  CCCCACCCCCTGGCCACAGCGTCCCCGCCTTCTACTTCCCTTGCGGTCGACCCCCGCCCCGTCCCCAGGA
  CACGGAGGATGCCATCGCCCTCGTGGAGTGCGCGTTCGAGGGGCTGCCTCGTGGGAGGGCGGGTCTGGGC
  GACATGGCCGTGGTTGCCAAGGCCTGTGGCTGCCCTCTGTACTGGAAGGCCCCGCTGTTCTACGCTGCGG
  GCGGCGAGCGCACAGGCTCTGTATCCGTGCACATGTTCGTAGCTATGTGGCGCAAAGTCCTGCTGACCTG
  CCATGATGATGCTGCGCGGTTCGTGCGGCTGCTGGGCCACCCCGGGTGTAGTGGCCTCATCCAGGAGGAC
  TTTGTGCCCTTCCTGCAGGATGTGGTGAACTCGCACCCGGGCCTGGCCTTCCTGAGGGCAGCGAAGGACT
  TCCACTCACGTTACATCACCACAGTGATTCAGAGGATCTTCTACACAGTAAACCGGTCATGGTCAGGGAT
  GATCTCGCGTGAGGAGCTAAGGCGCAGCAGCTTCCTGCAGGCTGTGTCACAGTTGGAGGTAGAGCCTGAC
  ATCAACCGCATGACGTCGTTCTTCTCTTATGAGCATTTCTATGTCATCTACTGCAAGTTCTGGGAGCTCG
  ACCTCGACCGCGACCTCACCATTGACAGGAGCGACCTCGCACGCCATGGGGACGGGGCCATCTCCTCCAG
  AATGATTGACCGCATCTTCTCAGGGGCCGTCACCAGGGCGAGGCTGCCCCGAAAGGTCGGGAAGCTGAGC
  TACGCAGACTTTGTATGGTTCCTGCTCTCCGAGGAGGACAAGACTACGCCCACCAGCACCGAGTACTGGT
  TCCGTTGCATGGACCTGGACGGTGACGGCGCTCTCTCCATGTTTGAGCTCGAGTTCTTCTATGAGGAGCA
  GGCCCAGCGCATGGCCGCCCGGGGTGTGGAGCCCCTCCCCTTCCACGACCTTGCACGCCAGGTGCTCGAC
  CTCGTCGCCCCGCGCTGCCCCGGCCGGATCACGCTGCGCGACCTGAAGCAGTGCGGCCTGGCGGGTGAGT
  TCTTCGACGCCTTCTTCAACGTGGACAAGTACCTGGCACGGGAGCAGCGCGAGCAGGCGGGGACTCCGCA
  GGACACTGACTCCGACCCCGCGGCATCTGCCTGGGACAGGTATGCCGCCGAGGAGTACGACTTCCTCGTG
  GCCGAGGAGGCCATGGCAGAGGACGACGACGACCATGACGAAGGGTCCGACCCCATAGATCTCTACGGCC
  TTGCTGACGAGGACTGCGACGACCTGGAGCCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208433 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPLTPRLQLKVDELFRRWLGDPHTQRALSHALRRIRDPGTTSDPAAAATPDADPGNTTSDPNPASRPLP
  RPALRTTGPRTVHAHRKEEPPPQSPPPGHSVPAFYFPCGRPPPRPQDTEDAIALVECAFEGLPRGRAGLG
  DMAVVAKACGCPLYWKAPLFYAAGGERTGSVSVHMFVAMWRKVLLTCHDDAARFVRLLGHPGCSGLIQED
  FVPFLQDVVNSHPGLAFLRAAKDFHSRYITTVIQRIFYTVNRSWSGMISREELRRSSFLQAVSQLEVEPD
  INRMTSFFSYEHFYVIYCKFWELDLDRDLTIDRSDLARHGDGAISSRMIDRIFSGAVTRARLPRKVGKLS
  YADFVWFLLSEEDKTTPTSTEYWFRCMDLDGDGALSMFELEFFYEEQAQRMAARGVEPLPFHDLARQVLD
  LVAPRCPGRITLRDLKQCGLAGEFFDAFFNVDKYLAREQREQAGTPQDTDSDPAASAWDRYAAEEYDFLV
  AEEAMAEDDDDHDEGSDPIDLYGLADEDCDDLEPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC027415
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC027415.1
• RefSeq Size: 1907 bp
• RefSeq ORF: 1577 bp
• Locus ID: 19054
• MW: 59.1 kDa