Asic1 (NM_009597) Mouse Tagged ORF Clone

CAT#: MR208438

Asic1 (Myc-DDK-tagged) - Mouse amiloride-sensitive cation channel 2, neuronal (Accn2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asic1
• Synonyms: Accn2; AI843610; ASIC; ASIC1a; B530003N02Rik; BNaC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208438 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGAAGACCGAGGAGGAGGAGGTGGGTGGTGTCCAGCCGGTGAGCATCCAGGCTTTTGCCAGCA
  GCTCCACGCTGCACGGTCTTGCCCACATCTTCTCCTATGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGC
  CCTGTGTTTCCTGGGTTCGCTGGCCGTCCTGCTGTGTGTGTGCACTGAGCGTGTGCAGTACTACTTCTGC
  TACCACCACGTCACCAAGCTCGACGAGGTGGCTGCCTCCCAGCTCACCTTCCCTGCCGTCACTCTCTGCA
  ACCTCAATGAGTTTCGCTTTAGCCAAGTCTCCAAGAATGACCTGTACCATGCTGGGGAACTGCTGGCCCT
  GCTCAACAACAGGTATGAGATACCGGACACACAGATGGCTGATGAAAAGCAGCTGGAGATATTGCAGGAC
  AAGGCCAACTTCCGTAGCTTCAAGCCCAAGCCCTTCAACATGCGTGAGTTCTACGACAGAGCAGGGCATG
  ACATTCGAGACATGCTTCTCTCGTGCCACTTCCGAGGGGAGGCCTGCAGCGCTGAAGACTTCAAAGTGGT
  CTTCACGCGGTATGGGAAGTGCTACACATTCAACTCGGGCCAAGATGGGCGGCCACGGCTGAAGACCATG
  AAAGGTGGGACTGGCAACGGCCTGGAGATCATGCTGGACATTCAGCAAGATGAATACTTGCCTGTGTGGG
  GAGAGACTGATGAGACATCGTTCGAAGCAGGCATCAAAGTGCAGATCCACAGTCAGGACGAGCCTCCTTT
  CATCGACCAGCTGGGCTTTGGCGTGGCCCCAGGCTTCCAGACGTTTGTGTCTTGCCAGGAGCAGAGGCTC
  ATCTACCTGCCCTCCCCCTGGGGCACCTGCAATGCTGTTACCATGGACTCGGATTTCTTCGACTCCTACA
  GCATCACGGCCTGCCGGATTGATTGTGAAACCCGTTACCTGGTGGAAAACTGCAACTGCCGTATGGTGCA
  CATGCCAGGGGATGCCCCATACTGTACTCCGGAGCAGTACAAGGAGTGTGCAGACCCTGCCCTGGACTTC
  CTAGTGGAGAAAGACCAGGAATACTGTGTGTGTGAGATGCCCTGCAACCTGACCCGCTACGGCAAGGAGC
  TGTCCATGGTCAAGATCCCCAGCAAAGCCTCAGCCAAGTACCTGGCCAAGAAGTTCAACAAATCTGAACA
  GTACATAGGGGAGAATATTCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATCGAGCAG
  AAGAAGGCCTATGAGATCGCAGGGCTTTTGGGTGACATCGGGGGCCAGATGGGATTGTTCATCGGGGCCA
  GCATCCTCACAGTGCTGGAACTCTTTGACTATGCCTATGAGGTCATTAAGCACCGGCTGTGTAGACGTGG
  GAAGTGCCAGAAGGAGGCTAAGAGGAACAGCGCAGATAAGGGCGTGGCGCTCAGCCTGGATGACGTCAAA
  AGACACAATCCCTGCGAGAGCCTCCGAGGACATCCTGCCGGGATGACGTACGCTGCCAACATCCTACCTC
  ACCATCCCGCTCGAGGCACGTTTGAGGACTTTACCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208438 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELKTEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFC
  YHHVTKLDEVAASQLTFPAVTLCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQD
  KANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEACSAEDFKVVFTRYGKCYTFNSGQDGRPRLKTM
  KGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVSCQEQRL
  IYLPSPWGTCNAVTMDSDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDF
  LVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKFNKSEQYIGENILVLDIFFEVLNYETIEQ
  KKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHRLCRRGKCQKEAKRNSADKGVALSLDDVK
  RHNPCESLRGHPAGMTYAANILPHHPARGTFEDFTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009597
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009597.1, NP_033727.1
• RefSeq Size: 3800 bp
• RefSeq ORF: 1581 bp
• Locus ID: 11419
• MW: 59.7 kDa