Cnot2 (BC063105) Mouse Tagged ORF Clone

CAT#: MR208509

Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot2
• Synonyms: AW557563
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208509 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAAGAAGTTGCACAGGTGACAAACAGCATGTTTGGTGCTTCAAGAAAGAAGTTTGTAGAGGGGG
  TGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTCTTCTATGTTCCCACATCGGTCAGAGAA
  AGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAA
  AGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAG
  GCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATC
  TCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATT
  GGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCA
  TAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAAT
  GAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGT
  GAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCG
  GCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGC
  AAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAA
  GATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAG
  ATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCA
  GGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGC
  CTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAA
  CAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTC
  ACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGAT
  AAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCG
  GGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCG
  GGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTAC
  TATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAG
  AGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCAGCAAGCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208509 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLKEVAQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQ
  SALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGI
  GIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGS
  ENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYK
  DPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIG
  LLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRD
  KLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTY
  YFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC063105
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063105, AAH63105
• RefSeq Size: 3014 bp
• RefSeq ORF: 1595 bp
• Locus ID: 72068
• MW: 58.6 kDa