Cnot2 (BC063105) Mouse Tagged ORF Clone

CAT#: MR208509

  • TrueORF®

Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026)


  "BC063105" in other vectors (4)


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Reconstitution Protocol

USD 320.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Cnot2
Synonyms AW557563
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAAAGAAGTTGCACAGGTGACAAACAGCATGTTTGGTGCTTCAAGAAAGAAGTTTGTAGAGGGGG
TGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTCTTCTATGTTCCCACATCGGTCAGAGAA
AGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAA
AGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAG
GCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATC
TCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATT
GGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCA
TAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAAT
GAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGT
GAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCG
GCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGC
AAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAA
GATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAG
ATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCA
GGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGC
CTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAA
CAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTC
ACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGAT
AAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCG
GGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCG
GGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTAC
TATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAG
AGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCAGCAAGCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208509 protein sequence
Red=Cloning site Green=Tags(s)

MLKEVAQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQ
SALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGI
GIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGS
ENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYK
DPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIG
LLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRD
KLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTY
YFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC063105
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC063105, AAH63105
RefSeq Size 3014 bp
RefSeq ORF 1595 bp
Locus ID 72068
MW 58.6 kDa

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