Eya2 (NM_010165) Mouse Tagged ORF Clone

CAT#: MR208530

Eya2 (Myc-DDK-tagged) - Mouse eyes absent 2 homolog (Drosophila) (Eya2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eya2
• Synonyms: Eab1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208530 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAAGTGGTGACCTCACCCAGCCTCGCAACAAGCAGTGACTGGAGCGAGCACGGTGCTGCCGTGG
  GGACGCTGAGTGACAGGGAAGGCATCGCCAAATCAGCGGCTCTGAGTGTGCCTCAGCTCTTTGTGAAGTC
  TCATCCACGTGTCCCTCCTGGTCAGTCCTCCACAGCCATGGCGGCCTATGGCCAGACACAGTACAGCACA
  GGCATTCAGCAGGCACCACCCTATACAGCGTACCCAACTCCGGCGCAAGCCTATGGAATCCCCCCTTACA
  GCATCAAGACAGAAGACAGTTTGAATCACTCCCCCAGCCAGAGCGGGTTCCTGAGCTACGGACCGAGCTT
  CAGCACCGCGCCTGCTGGACAGAGCCCCTACACCTACCCCGTGCACAGCACCGCTGGGCTCTATCAAGGC
  GCCAACGGACTGACCAACACCGCTGGATTTGGGAGCGTGCACCAGGATTATCCGTCCTACCCCAGCTTTT
  CACAGAACCAGTACCCCCAGTATTTCAGCCCATCATACAACCCGCCCTACGTCCCTGCCAGCAGCCTCTG
  CTCCTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCTCCTCACAATGTCCCCAGCCAGAGTTCT
  GAGTCCCTGGCCGGAGACTACAACACACACAACGGACCCTCCACACCAGCAAAGGAGGGTGACACAGAGA
  GGCCACATCGAGCCTCGGATGGGAAGCTACGGGGCCGGTCAAAGAGAAATAGTGACCCTTCCCCAGCAGG
  AGACAATGAAATCGAGCGCGTGTTCGTCTGGGACCTGGACGAGACAATCATTATCTTCCACTCCCTGCTC
  ACAGGGACGTTTGCATCCAGATACGGGAAGGACACCACGACGTCTGTGCGCATTGGCCTGATGATGGAGG
  AGATGATCTTCAACCTTGCTGACACACACCTGTTCTTCAATGACCTGGAGGACTGTGACCAAATCCACGT
  GGATGATGTCTCATCCGATGACAATGGTCAGGATTTAAGCACATACAACTTCTCCACTGATGGCTTCCAC
  AGCACGGCGCCAGGAGCCAGCTTGTGCCTGGGTACAGGTGTTCATGGCGGTGTGGACTGGATGAGGAAAC
  TGGCCTTCCGCTACCGTCGTGTGAAGGAGATGTACAACACCTACCGCAACAACGTGGGTGGCTTGATAGG
  TGCTCCCAAAAGAGAGACCTGGCTGCAGCTGCGCGCCGAGCTGGAGGCCCTGACTGACCTCTGGCTCACC
  CACTCCCTGAAAGCCCTCAATCTCATCAACTCTCGACCCAACTGTGTCAATGTGTTGGTCACCACCACGC
  AACTGATCCCTGCATTGGCCAAGGTCCTGCTGTACGGTCTGGGCTCCGTGTTCCCCATCGAGAACATCTA
  CAGTGCGACCAAGACAGGCAAGGAGAGCTGCTTCGAAAGAATCATGCAGAGGTTTGGCCGCAAAGCTGTC
  TACATTGTGATAGGCGACGGGGTAGAGGAAGAGCAAGGAGCCAAAAAGCACAACATGCCTTTCTGGAGGA
  TATCCTGTCATGCTGACCTGGAGGCTCTAAGGCATGCCCTGGAACTGGAGTATCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208530 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLEVVTSPSLATSSDWSEHGAAVGTLSDREGIAKSAALSVPQLFVKSHPRVPPGQSSTAMAAYGQTQYST
  GIQQAPPYTAYPTPAQAYGIPPYSIKTEDSLNHSPSQSGFLSYGPSFSTAPAGQSPYTYPVHSTAGLYQG
  ANGLTNTAGFGSVHQDYPSYPSFSQNQYPQYFSPSYNPPYVPASSLCSSPLSTSTYVLQEAPHNVPSQSS
  ESLAGDYNTHNGPSTPAKEGDTERPHRASDGKLRGRSKRNSDPSPAGDNEIERVFVWDLDETIIIFHSLL
  TGTFASRYGKDTTTSVRIGLMMEEMIFNLADTHLFFNDLEDCDQIHVDDVSSDDNGQDLSTYNFSTDGFH
  STAPGASLCLGTGVHGGVDWMRKLAFRYRRVKEMYNTYRNNVGGLIGAPKRETWLQLRAELEALTDLWLT
  HSLKALNLINSRPNCVNVLVTTTQLIPALAKVLLYGLGSVFPIENIYSATKTGKESCFERIMQRFGRKAV
  YIVIGDGVEEEQGAKKHNMPFWRISCHADLEALRHALELEYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010165
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010165.1, NM_010165.2, NM_010165.3, NP_034295.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 1599 bp
• Locus ID: 14049
• MW: 58.2 kDa
• Gene Summary: This gene encodes a member of the eyes absent protein family. The encoded protein is a tyrosine phosphatase which acts as a transcriptional activator during development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]