Aldh6a1 (NM_134042) Mouse Tagged ORF Clone

CAT#: MR208569

Aldh6a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 6, subfamily A1 (Aldh6a1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh6a1
• Synonyms: 1110038I05Rik; Mmsdh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGTGGCGGCAGCGGCAGCAATGCGGTCCCGGATCCTGCAGGTTTCTTCTAAGGTAAATG
  CCACATGGTATCCAGCATCCTCCTTCTCTTCCTCATCAGTGCCAACTGTAAAGCTCTTCATTGATGGAAA
  ATTTGTTGAATCAAAAAGTGATAAATGGATTGACATCCACAACCCAGCCACCAATGAAGTTGTTGGTAGG
  GTTCCTCAGTCCACCAAAGCTGAGATGGACGCAGCCGTTGAGTCCTGCAAACGTGCTTTCCCTGCGTGGG
  CAGACACATCTATTTTAAGCCGGCAGCAGGTCCTGCTTCGTTATCAGCAGCTCATTAAAGAAAACCTGAA
  AGAAATTGCCAGGTTAATCACACTGGAACAAGGGAAGACTCTTGCTGATGCTGAAGGAGATGTATTCCGA
  TGCCTTCAGGTGGTTGAGCATGCCTGTAGTGTGACATCCCTCATGCTGGGAGAGACCATGCCATCTATCA
  CCAAAGACATGGACCTTTATTCCTACCGTCTGCCTCTGGGGGTGTGTGCAGGCATTGCACCATTTAATTT
  TCCTGCCATGATCCCCCTTTGGATGTTTCCCATGGCTATGGTTTGTGGAAATACTTTCCTAATGAAACCA
  TCAGAGCGAGTACCTGGAGCAACTATGCTCCTTGCTAAGTTGCTTCAGGATTCTGGGGCCCCTGACGGAA
  CACTGAACATTATCCATGGACAACATGATGCTGTAAACTTTATTTGCGATCATCCAGACATCAAGGCAAT
  CAGCTTTGTGGGATCCAACCAGGCAGGAGAGTACATCTTCGAAAGAGGATCAAGAAATGGCAAGAGGGTT
  CAAGCCAATATGGGAGCCAAAAACCATGGAGTAGTCATGCCAGACGCCAATAAGGAAAATACCCTCAACC
  AGCTGGTCGGGGCAGCATTCGGAGCTGCTGGGCAGCGCTGCATGGCTCTTTCAACAGCCATCCTCGTAGG
  GGAGGCTAAGAAGTGGCTGCCAGAACTGGTCGATCGGGCCAAAAATCTGCGAGTCAATGCAGGAGACCAG
  CCTGGAGCTGATCTTGGCCCCCTGATCACCCCCCAGGCCAAAGAGAGAGTCTGCAATCTGATCGATAGCG
  GAACAAAAGAGGGAGCTTCCATCCTTCTGGATGGGCGAAGAATTAAAGTCAAGGGCTATGAAAATGGTAA
  CTTTGTTGGACCAACCATCATCTCAAATGTTAAGCCAAGTATGACTTGTTACAAAGAGGAGATATTTGGT
  CCAGTTCTTGTGGTTTTGGAGACAGAAACACTGGATGAAGCAATCAAGATTGTAAATGACAACCCATATG
  GAAATGGAACTGCCATCTTCACCACCAATGGAGCCACCGCCCGGAAATATGCTCACATGGTGGATGTGGG
  ACAGGTGGGTGTGAATGTCCCCATTCCAGTGCCTTTGCCGATGTTTTCCTTCACCGGCTCTCGCTCTTCC
  TTCAGGGGAGACACCAATTTCTATGGCAAACAGGGCATCCAGTTTTACACGCAGTTAAAGACTATCACGT
  CTCAGTGGAAAGAAGAAGATGCTACGCTTTCCTCCCCTGCTGTAGTCATGCCTACCATGGGGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVAAAAAMRSRILQVSSKVNATWYPASSFSSSSVPTVKLFIDGKFVESKSDKWIDIHNPATNEVVGR
  VPQSTKAEMDAAVESCKRAFPAWADTSILSRQQVLLRYQQLIKENLKEIARLITLEQGKTLADAEGDVFR
  CLQVVEHACSVTSLMLGETMPSITKDMDLYSYRLPLGVCAGIAPFNFPAMIPLWMFPMAMVCGNTFLMKP
  SERVPGATMLLAKLLQDSGAPDGTLNIIHGQHDAVNFICDHPDIKAISFVGSNQAGEYIFERGSRNGKRV
  QANMGAKNHGVVMPDANKENTLNQLVGAAFGAAGQRCMALSTAILVGEAKKWLPELVDRAKNLRVNAGDQ
  PGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRRIKVKGYENGNFVGPTIISNVKPSMTCYKEEIFG
  PVLVVLETETLDEAIKIVNDNPYGNGTAIFTTNGATARKYAHMVDVGQVGVNVPIPVPLPMFSFTGSRSS
  FRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPTMGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134042
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_134042.1, NM_134042.2, NM_134042.3, NP_598803.1
• RefSeq Size: 3346
• RefSeq ORF: 1608
• Locus ID: 104776
• MW: 58 kDa
• Gene Summary: This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded enzyme is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This enzyme catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Mutations in the human gene result in Methylmalonate Semialdehyde Dehydrogenase Deficiency, characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]