Cpne1 (NM_170590) Mouse Tagged ORF Clone

CAT#: MR208584

Cpne1 (Myc-DDK-tagged) - Mouse copine I (Cpne1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpne1
• Synonyms: 1810028N16Rik; mKIAA4108
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208584 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCATTGCGTGACCTTGGTCCAGCTCTCCGTGTCCTGTGAACATCTCATTGACAAGGACATTGGCT
  CCAAGTCTGACCCACTCTGCGTCCTTTTACAGGATGTGGGCGGGGCCTGGGCCGAGCCTTGCCGGACTGA
  ACGTGTTCGCAACTGCTCAAGCCCTGAGTTCTCCAAGACTCTGCAGATAGAATACCACTTTGAGACTGTC
  CAGAAGCTGCGCTTTGGAATCTATGACATAGACAACAAGACACCAGAGCTGGGGGATGACGACTTCCTGG
  GGGGAGCTGAATGTTCCCTAGGTCAGATTGTATCCAGCCAGACGCTGACCTTACCCTTGATGTTGAAGCC
  TGGGAAGCCTGCAGGGCGGGGAACCATCACAGTTTCAGCTCAGGAGCTAAAGGACAGCCGTGTAGTAACC
  ATGGAGGTGGAAGCTAGAAACCTAGATAAGAAGGACTTCCTAGGGAAATCTGACCCATTCTTGGAGTTCT
  TCCGGCAAGGCGATGGGAAGTGGCAGCTGGCATACAGAACCGAGGTAGTCAAGAACAACCTGAACCCTAC
  CTGGAAGCGCTTCTCAGTCTCTCTGCAGCATTTCTGTGGCGGGGACCTCAGCACGCCCATTCAGGTGCGC
  TGCTCAGACTATGACAGTGACGGCTCACATGATCTCATTGGTACCTTCCATACTACCTTGGCCCAACTGC
  AAGCAGTCCCGGCTGAGTTTGAATGTGTCCACCCTGAAAAGCAGCAGAGAAAGAAAAACTACAGGAACTC
  TGGAACTGTCCGTGTCAAGACTTGCCGGGTGGAGACAGAGTATTCCTTCCTGGACTATGTGATGGGAGGC
  TGCCAGATCAACTTTACTGTGGGTGTGGACTTCACTGGCTCCAATGGCGACCCATCCTCACCTGACTCCC
  TGCATTATCTGAGCCCTACAGGAGTCAACGAGTATCTGACAGCTCTGTGGAGCGTGGGCAGCGTGGTTCA
  AGACTATGACTCAGACAAGCTATTTCCTGCATTTGGTTTTGGAGCCCAGGTGCCCCCTGATTGGCAGGTC
  TCACATGAGTTTGCCTTGAACTTCAATCCCAGTAATCCGTATTGTGCAGGCATCCAGGGTATCGTCGATG
  CCTACCGTCAAGCCCTGCCCCAAGTTCGTCTCTATGGCCCCACCAACTTTGCACCCATCATCAACCACGT
  GGCCAGGTTTGCAGCCCAAGCTGCACAGCAGAGGTCGGCCTCGCAATACTTCGTGCTGTTGCTTTTGACT
  GACGGTGCTGTGACAGATGTGGAGGCCACATGTAAGGCAGTGGTGGACGCCTCAAAGCTGCCCATGTCCG
  TGATCATCGTGGGTGTGGGCGGTGCGGATTTTGAGGTCATGGAACAGCTGGATGCAGATGGTGGCCCTCT
  CCGCACGCGCTCTGGAGAAGCAGCGGCCCGAGACATAGTGCAGTTCGTGCCTTATCGACGATTTCAGAAT
  GCTCCCCGGGAGACACTCGCACAGACCGTACTCGCCGAAGTGCCCACTCAGATGGTTTCCTACTTCAGAG
  CCCAAGGCTGGGCCCCGTTAAAGGCACCTCCAACCCCAGGCAAGGGCCCCGCACAGGCCCCACAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208584 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHCVTLVQLSVSCEHLIDKDIGSKSDPLCVLLQDVGGAWAEPCRTERVRNCSSPEFSKTLQIEYHFETV
  QKLRFGIYDIDNKTPELGDDDFLGGAECSLGQIVSSQTLTLPLMLKPGKPAGRGTITVSAQELKDSRVVT
  MEVEARNLDKKDFLGKSDPFLEFFRQGDGKWQLAYRTEVVKNNLNPTWKRFSVSLQHFCGGDLSTPIQVR
  CSDYDSDGSHDLIGTFHTTLAQLQAVPAEFECVHPEKQQRKKNYRNSGTVRVKTCRVETEYSFLDYVMGG
  CQINFTVGVDFTGSNGDPSSPDSLHYLSPTGVNEYLTALWSVGSVVQDYDSDKLFPAFGFGAQVPPDWQV
  SHEFALNFNPSNPYCAGIQGIVDAYRQALPQVRLYGPTNFAPIINHVARFAAQAAQQRSASQYFVLLLLT
  DGAVTDVEATCKAVVDASKLPMSVIIVGVGGADFEVMEQLDADGGPLRTRSGEAAARDIVQFVPYRRFQN
  APRETLAQTVLAEVPTQMVSYFRAQGWAPLKAPPTPGKGPAQAPQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170590
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170590.1, NM_170590.2, NM_170590.3, NP_733469.1
• RefSeq Size: 3493 bp
• RefSeq ORF: 1611 bp
• Locus ID: 266692
• MW: 58.9 kDa
• Gene Summary: This gene encodes a protein that contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. Its activity is also upregulated in mouse embryos. This gene and the gene for RNA binding motif protein 12 overlap at map location 2 H2. Two alternatively spliced variants that encode the same isoform have been identified for this gene. [provided by RefSeq, Jul 2008]