Cnot2 (NM_001037847) Mouse Tagged ORF Clone

CAT#: MR208625

Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (Cnot2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot2
• Synonyms: 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208625 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAGGACTGATGGACATACATTATCTGAGAAAAGAAACTACCAGGTGACAAACAGCATGTTTGGTG
  CTTCAAGAAAGAAGTTTGTAGAGGGGGTGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTC
  TTCTATGTTCCCACATCGGTCAGAGAAAGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAA
  TTTGGGGCAAGTTTATACGGGCAACAAAGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCC
  CTCAGTTAAATCGCAGCTTATCACAAGGCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACC
  AACAATGTCACTTCACACGCCTCCATCTCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATG
  AACCACTCCCAGGTTGGTCAGGGCATTGGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAG
  GTAGCCCCAACAGAAGCTCGCCAAGCATAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCCTTTAC
  TGTGAACAGTATGTCTGGATTTGGAATGAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGT
  AACATTTTTAATGGAACAGATGGCAGTGAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAG
  CTGACCGGAATAGAAGGGAAGGGAGCGGCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCC
  TTACGTTGGAATGGTAACAAAACCTGCAAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTT
  CCAGCATTACCTGGTTCCAGCTATAAAGATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTA
  CATCGGGGAAGACGACTTCAAGTACAGATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAA
  CAATAATCAACAGAAAAAGGGGATCCAGGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATG
  GTGACGGACCAATTTGGAATGATTGGCCTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGG
  TACATCTTGCTTTAGGAAGTGACTTAACAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCC
  CAAATTTGCATCACCCTGGGCTTCTTCACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAA
  TATTTAACAAACATTCACATTAGGGATAAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCC
  TCTTCTATCTGTATTACATGAACGGCGGGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCG
  TGATTGGAGATACCACAAGGAGGAGCGGGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAA
  ACCAACACGTATGAGCGGGGGACCTACTATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGT
  TCCATCTGGAATATGACAAATTAGAAGAGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCA
  GCAAGCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208625 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQ
  FGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMM
  NHSQVGQGIGIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSS
  NIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDF
  PALPGSSYKDPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGM
  VTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSE
  YLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMK
  TNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037847
• ORF Size: 1623 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037847.1, NP_001032936.1
• RefSeq Size: 2746 bp
• RefSeq ORF: 1653 bp
• Locus ID: 72068
• Cytogenetics: 10 D2
• MW: 59.7 kDa