Ftcd (NM_080845) Mouse Tagged ORF Clone

CAT#: MR208640

Ftcd (Myc-DDK-tagged) - Mouse formiminotransferase cyclodeaminase (Ftcd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ftcd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208640 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGTTGGTGGAATGTGTCCCCAACTTCTCAGAGGGGAACAACCAGGAGGTGATTGATGCCATCT
  CTCGAGCCATCTCCCAGACCCCAGGCTGTGTGCTGTTGGACGTCGATGCCGGACCCTCCACCAACCGCAC
  TGTCTACACTTTCGTGGGGCAGCCAGAGTGTGTCGTAGAGGGGGCCCTCCATGCTGCGCGTACGGCCTCA
  CAGCTCATCGACATGAGCAAGCACAAGGGTGAGCACCCTCGAATGGGCGCTCTGGATGTGTGTCCCTTCA
  TCCCGGTGAGGGGCGTCAGCATGGAAGAGTGTGTGCTCTGTGCCAAGGCATTTGGGCAGCGGCTGGCAGA
  GGAACTTAATGTACCAGTGTATCTCTATGGTGAGGCAGCTCAGACGCCCAGTCGTCAGACCCTGCCAGCC
  ATCCGGGCTGGAGAGTATGAGGCCCTGCCTGAGAAGCTCAAACAGGCCGAGTGGGTGCCAGACTTTGGCC
  CTAGCTCCTTTGTCCCCAGTTGGGGTGCTACTGTCACGGGTGCACGGAAGTTCCTCATTGCATTCAATAT
  CAACCTGCTAAGCACCAAGGAGCAGGCCCACCGTATTGCTCTTAACCTGCGGGAGCAGGGCCGAGGGAAA
  GACCAGCCTGGACGTCTGAAAAAGGTCCAGGGCATCGGTTGGTACCTGGAAGAGAAGAACCTGGCTCAGG
  TGTCCACAAACCTCCTGGACTTTGAGGTCACAGCTCTGCACACAGTGTTTGAGGAGGCCCGCAGAGAGGC
  TCAGGAGCTGAATCTCCCAGTGGTGGGCTCGCAGCTGGTGGGCCTGGTGCCTCTGAAGGCCTTACTGGAT
  GCCGCAGCCTTCTACTGTGACAAGGAGAAGCTGTTTGTTCTGGAGGAGGAGCATCGGATACGGCTGGTGG
  TGAACCGGCTGGGCCTGGATTCGCTGGCACCCTTCGACCCAAAGGAACGGATCATCGAGTACCTAGTCCC
  CGATAGTGGACCAGAACAGAGCCTGTTGGACACCTCACTGCGCGGTTTTGTTCGGGAGGTGGGAGCCCGC
  TCTGCTGCCCCAGGAGGAGGTTCTGTAGCAGCAGCGGTAGCAGCTCTGGGTGCAGCCCTGGCTTCTATGG
  TGGGCCAGATGACCTATGGCCGGCGCCAGTTTGATCACTTGGATTCCACAATGCGACGCCTGATCCCACC
  CTTCCACGCAGCCTCTGCCCAGCTGACTTCATTGGTGGATGCTGATGCCCGGGCGTTTGCTGCCTGTTTG
  GAGGCAATAAAGCTGCCCAAGAACACGCCTGAAGAGAGAGACAGGCGTGCATGCGCTCTGCAGGAGGGGC
  TGAGACAGGCAGTCGCTGTGCCACTGAAACTGGCCGAGACTGTGTCCCAGCTATGGCCAGCGCTGCAGGA
  GCTGGCCCACTGTGGGAACCTGTCCTGCTTGTCTGACCTGCAGGTGGCAGCCAAAGCCTTGGAGACAGGT
  GTGTTTGGTGCGTACTTCAACGTGCTCATCAACCTGAAGGACATGACGGATGATGTATTTAAAGAGAAGA
  CACACCATCGAATCTCCAGCCTTCTGCAGGAAGCCAAGACTCAGGCTGCGCTGGTGCTGGGTAGCCTAGA
  AGCCCGAAAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208640 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQLVECVPNFSEGNNQEVIDAISRAISQTPGCVLLDVDAGPSTNRTVYTFVGQPECVVEGALHAARTAS
  QLIDMSKHKGEHPRMGALDVCPFIPVRGVSMEECVLCAKAFGQRLAEELNVPVYLYGEAAQTPSRQTLPA
  IRAGEYEALPEKLKQAEWVPDFGPSSFVPSWGATVTGARKFLIAFNINLLSTKEQAHRIALNLREQGRGK
  DQPGRLKKVQGIGWYLEEKNLAQVSTNLLDFEVTALHTVFEEARREAQELNLPVVGSQLVGLVPLKALLD
  AAAFYCDKEKLFVLEEEHRIRLVVNRLGLDSLAPFDPKERIIEYLVPDSGPEQSLLDTSLRGFVREVGAR
  SAAPGGGSVAAAVAALGAALASMVGQMTYGRRQFDHLDSTMRRLIPPFHAASAQLTSLVDADARAFAACL
  EAIKLPKNTPEERDRRACALQEGLRQAVAVPLKLAETVSQLWPALQELAHCGNLSCLSDLQVAAKALETG
  VFGAYFNVLINLKDMTDDVFKEKTHHRISSLLQEAKTQAALVLGSLEARKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080845
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080845.1, NM_080845.2, NP_543121.1
• RefSeq Size: 2710 bp
• RefSeq ORF: 1626 bp
• Locus ID: 14317
• MW: 58.9 kDa