Ajuba (NM_010590) Mouse Tagged ORF Clone

CAT#: MR208706

Ajuba (Myc-DDK-tagged) - Mouse ajuba (Jub)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ajuba
• Synonyms: Jub
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208706 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGGTTAGGGGAGAAAGCCAGTCGTCTGCTAGAAAAGTTGAGACTCTCGGACTCCGGCAGCGCCA
  AGTTCGGCCGCAGAAAGGGCGAAGCGAGCCGATCTGGGTCTGATGGGACCCCCGGAGCAGGCAAGGGACG
  CTTAAGCGGGTTGGGGGGACCTAGGAAGTCAGGACACCGTGGAGCGAATGGTGGGCCTGGAGATGAACCT
  TTGGAACCGGCCAGGGAGCAAGGGCCCCTGGACGCCGAGCGGAACGCACGCGGCTCCTTTGAAGCGCAGC
  GCTTCGAAGGGTCCTTTCCCGGGGGGCCGCCGCCCACTCGAGCCCTGCCTCTGCCTCTGTCGTCGCCTCC
  TGATTTTCGGCTGGAGACCACGGCTCCAGCCCTTAGCCCTCGCTCCAGCTTCGCCAGTAGCTCGGCCAGC
  GATGCGAGCAAGCCATCTAGCCCCCGGGGCAGCCTGCTGCTGGACGGAGCGGGGGCCAGCGGAGCCGGAG
  GTAGCCGGCCGTGCAGCAATCGGACCAGCGGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCGCT
  GCCCGCGGGGCCGTGCCTATTCGGCCTCCCGCTGACCACTGCTCCTGCGGGCTACCCAGGAGGGGCTCCG
  TCCGCCTACCCGGAGCTCCACGCTGCCCTGGACCGACTATGTGCTCATCGGTCCGTGGGATTCGGCTGCC
  AGGAGAGCCGTCACTCGTACCCCCCGGCCCTGGGCAGCCCCGGAGCTCTAACCGGAGCCGTGGTGGGAAC
  AGCGGGTCCTTTGGAGAGACGTGGGGCGCAACCCGGACGACACTCGGTTACAGGCTACGGGGACTGCGCC
  GCGGGGGCCCGTTACCAGGACGAGCTAACAGCATTGCTGCGTTTGACCGTGGCTACCGGTGGGCGAGAAG
  CCGGTGCTCGCGGGGAACCCTCGGGGATTGAGCCGTCGGGTCTGGAGGAGTCTCCTGGTCCCTTCGTTCC
  AGAGGCCTCCCGATCACGGATACGGGAGCCAGAGGCCAGAGAAGATTACTTTGGCACCTGTATCAAGTGC
  AACAAAGGTATCTATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCACACCCAGTGCTTTG
  TCTGCTGCTCCTGTGGACGAACTTTGCGGTGCAAGGCTTTCTACAGCGTCAATGGCTCTGTCTACTGTGA
  GGAAGACTATCTGTTTTCAGGGTTTCAGGAGGCAGCTGAGAAGTGCTGTGTCTGTGGCCACTTGATTCTA
  GAGAAGATCCTCCAGGCCATGGGGAAGTCCTATCACCCGGGCTGCTTCCGATGCATCGTATGTAACAAGT
  GCCTGGACGGCGTCCCCTTCACTGTGGACTTCTCCAACCAGGTGTACTGTGTTACCGACTACCACAAAAA
  TTACGCCCCGAAATGTGCAGCCTGCGGACAACCCATCCTCCCCTCAGAGGGCTGTGAGGACATTGTGAGG
  GTGATATCCATGGACCGTGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGCATGCAGCTGAGTG
  ACGAGGAAGGTTGCTGCTGTTTCCCTCTTGATGGACATTTGCTCTGCCACGGCTGTCACATGCAGCGCCT
  CAGTGCCCGGCAGCCCTCTACCAACTATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208706 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAGKGRLSGLGGPRKSGHRGANGGPGDEP
  LEPAREQGPLDAERNARGSFEAQRFEGSFPGGPPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSAS
  DASKPSSPRGSLLLDGAGASGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPAGYPGGAP
  SAYPELHAALDRLCAHRSVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGAQPGRHSVTGYGDCA
  AGARYQDELTALLRLTVATGGREAGARGEPSGIEPSGLEESPGPFVPEASRSRIREPEAREDYFGTCIKC
  NKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLIL
  EKILQAMGKSYHPGCFRCIVCNKCLDGVPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVR
  VISMDRDYHFECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPSTNYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010590
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010590.1, NM_010590.2, NM_010590.3, NM_010590.4, NM_010590.5, NP_034720.2
• RefSeq Size: 3503 bp
• RefSeq ORF: 1644 bp
• Locus ID: 16475
• MW: 57.9 kDa