Dab2 (NM_001008702) Mouse Tagged ORF Clone

CAT#: MR208713

Dab2 (Myc-DDK-tagged) - Mouse disabled homolog 2 (Drosophila) (Dab2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dab2
• Synonyms: 5730435J12Rik; AA960054; AI957090; D15Wsu122e; D630005B22Rik; Doc-2; Doc2; p96
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208713 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAACGAAGTAGAAACAAGCACAGCCAATGGTCAGCCTGACCAACAGGCTGCCCCGAAAGCACCAT
  CAAAGAAGGAGAAGAAGAAAGGTTCTGAAAAGACAGACGAGTACTTGTTGGCCAGGTTCAAAGGTGATGG
  TGTAAAATACAAGGCCAAGCTAATCGGTATTGATGATGTGCCTGATGCTCGAGGAGACAAAATGAGCCAG
  GATTCTATGATGAAACTCAAGGGAATGGCAGCAGCTGGTCGCTCTCAGGGACAACACAAGCAAAGAATCT
  GGGTCAACATTTCCTTGTCTGGCATAAAAATCATTGATGAGAAAACTGGGGTAATTGAACATGAACATCC
  AGTAAATAAGATTTCCTTCATTGCTCGTGATGTGACAGACAACAGAGCATTTGGTTATGTGTGTGGAGGT
  GAAGGCCAGCATCAATTTTTTGCTATAAAAACAGGGCAACAGGCTGAACCATTAGTCGTCGATCTTAAAG
  ACCTTTTTCAAGTTATCTATAATGTAAAGAAAAAGGAAGAAGATAAGAAAAAGGTTGAAGAAGCCAACAA
  AGCAGAAGAGAATGGAAGTGAGGCCCTAATGACCCTTGATGATCAAGCTAACAAATTGAAGCTGGGTGTT
  GACCAGATGGATTTGTTTGGGGACATGTCTACACCTCCTGGCCTAAATAGTCCAACATCTTCAGCAAACG
  ACTTGCTTGCTTCAGACATCTTTGCCTCAGAACCTCCAGGCCAGATGTCCCCCACAGGACAACCTGCAGT
  CCCGCAGTCGAACTTTCTGGATCTCTTCAAAGGCAATGCTCCTGCCCCAGTGGGGCCCCTTGTAGGTCTA
  GGTACGGTCCCAGTAACACCCCCCCAAGCAGGACCCTGGACGCCTGTTGTCTACAGTCCTTCGACAACTG
  TGGTCCCAGGAGCCATAATAAGTGGCCAGCCTCCCAGTTTTGGCCAGCCACTCGTTTTTGGTACAACCCC
  AGCAGTACAAGTCTGGAATCAGCCTCCATCATTTGCAACCCCAGCTTCCCCTCCACCCCCCACAGTTTGG
  TGTCCTACCACATCTGTGGCGCCCAACGCTTGGTCATCCACAAGCCCTCTGGGGAATCCTTTTCAGAGTA
  ATAATATCTTTCCACCTCCCACCATGTCCACTCAGTCCTCTCCTCAGCCTATGATGTCCTCTGTTCTGGC
  CACACCGCCTCAACCACCTCCCCGAAATGGCCCACTAAAGGACATTCCCAGTGACGCTTTCACTGGCTTA
  GACCCCCTTGGGGATAAAGAGGTCAAAGAAGTGAAAGAAATGTTTAAGGACTTCCAGCTGCGGCAGCCAC
  CTCTTGTTCCCTCAAGGAAGGGGGAGACGCCTCCCTCTGGGACTTCAAGCGCCTTCTCCAGTTACTTCAA
  CAATAAAGTTGGCATTCCTCAGGAGCACGTAGACCATGATGATTTTGATGCCAATCAACTGTTGAACAAG
  ATTAATGAACCACCAAAGCCAGCCCCGAGACAAGGTGTCCTCTTGGGTACCAAGTCTGCTGACAATTCAC
  TTGAGAACCCTTTCTCCAAAGGGTTCAGCTCATCAAACCCCTCTGTGGTTTCTCAGCCTGCATCTTCTGA
  TCCCCACAGGAGCCCTTTCGGAAATCCTTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208713 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNEVETSTANGQPDQQAAPKAPSKKEKKKGSEKTDEYLLARFKGDGVKYKAKLIGIDDVPDARGDKMSQ
  DSMMKLKGMAAAGRSQGQHKQRIWVNISLSGIKIIDEKTGVIEHEHPVNKISFIARDVTDNRAFGYVCGG
  EGQHQFFAIKTGQQAEPLVVDLKDLFQVIYNVKKKEEDKKKVEEANKAEENGSEALMTLDDQANKLKLGV
  DQMDLFGDMSTPPGLNSPTSSANDLLASDIFASEPPGQMSPTGQPAVPQSNFLDLFKGNAPAPVGPLVGL
  GTVPVTPPQAGPWTPVVYSPSTTVVPGAIISGQPPSFGQPLVFGTTPAVQVWNQPPSFATPASPPPPTVW
  CPTTSVAPNAWSSTSPLGNPFQSNNIFPPPTMSTQSSPQPMMSSVLATPPQPPPRNGPLKDIPSDAFTGL
  DPLGDKEVKEVKEMFKDFQLRQPPLVPSRKGETPPSGTSSAFSSYFNNKVGIPQEHVDHDDFDANQLLNK
  INEPPKPAPRQGVLLGTKSADNSLENPFSKGFSSSNPSVVSQPASSDPHRSPFGNPFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008702
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008702.1, NM_001008702.2, NP_001008702.1
• RefSeq Size: 3792 bp
• RefSeq ORF: 1647 bp
• Locus ID: 13132
• Cytogenetics: 15 2.15 cM
• MW: 58.6 kDa