Gabra4 (NM_010251) Mouse Tagged ORF Clone

CAT#: MR208763

Gabra4 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4 (Gabra4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gabra4
• Synonyms: Gabra-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208763 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTGTCCAGAAGGTACCCGCGATTGCGCTGTGCTCCGGGGTCAGCCTCGCCCTCCTGCACTTCC
  TGTGCCTGGCGGCTTGTTTAAACGAATCCCCAGGACAGAACTCAAAGGACGAGAAATTGTGCCCGGAAAA
  TTTTACCCGTATTCTGGACAGTTTGCTGGATGGTTATGACAACAGGCTGCGTCCTGGATTTGGGGGTCCT
  GTTACAGAAGTGAAAACTGATATATATGTCACCAGCTTTGGGCCCGTTTCTGATGTTGAAATGGAATACA
  CTATGGATGTGTTCTTCAGACAGACATGGATTGACAAAAGACTAAAATATGACGGCCCAATTGAAATCTT
  GAGGCTGAATAATATGATGGTCACCAAAGTTTGGACCCCTGATACTTTCTTCAGGAATGGAAAGAAATCT
  GTCTCACATAACATGACAGCTCCAAATAAGCTTTTTAGAATTATGAGAAATGGCACTATTTTATACACAA
  TGAGACTCACCATAAGTGCGGAGTGCCCCATGAGACTGGTGGATTTTCCTATGGATGGTCATGCCTGCCC
  TTTGAAATTTGGGAGTTATGCATATCCCAAAAGTGAGATGATCTACACCTGGACCAAAGGCCCTGAGAAG
  TCAGTGGAGGTGCCAAAGGAGTCTTCTAGCTTAGTTCAATATGACCTCATTGGGCAGACTGTATCAAGCG
  AGACTATCAAATCTATTACAGGTGAATACATTGTTATGACGGTTTACTTCCACCTCAGACGGAAGATGGG
  CTACTTTATGATTCAGACGTATATCCCATGCATCATGACAGTGATTCTTTCTCAAGTTTCCTTCTGGATA
  AACAAGGAGTCTGTTCCAGCTAGAACTGTATTTGGAATAACCACAGTCCTCACGATGACCACCCTAAGCA
  TCAGTGCTCGGCATTCTTTGCCCAAAGTGTCCTATGCGACTGCCATGGATTGGTTCATAGCTGTCTGTTT
  TGCTTTTGTATTTTCGGCTCTTATTGAGTTTGCTGCTGTCAACTATTTCACCAACATTCAAATGCAGAAA
  GCCAAAAAGAAGATATCAAAGCCTCCCCCAGAAGTTCCAGCTGCTCCTGTGCTGAAGGAGAAACACACAG
  AAACATCCCTTCAGAATACACATGCCAATTTGAACATGAGGAAAAGAACAAATGCCTTGGTCCATTCAGA
  ATCGGATGTCAAAAGCAGAACTGAGGTGGGAAATCACTCCAGCAAGACCAGCGCTGTCCAGGAGTCTTCT
  GAAGCCACGCCTAAGGCTCACTTAGCTTCCAGTCCAAATCCATTCAGCAGGGCAAATGCAGCTGAGACTA
  TGTCTGCTGCAGCCAGAGGTCTTTCATCTGCAGCATCCCCCTCTCCTCATGGCACATTGCGGCCAGCTTC
  TTTGGGGTCAGCTTCCACTCGCCCTGCATTTGGATCTAGACTTGGGCGAATTAAGACAACAGTTAATACA
  ACAGGGGCTGCTGGGAATGTGTCAGCCACACCTCCTCCCCCTGCTCCACCGCCTTCTGGATCTGGCACAA
  GTAAAATAGACAAATATGCTCGTATTCTCTTTCCAGTCACATTTGGAGCATTTAACATGGTCTACTGGGT
  TGTTTATTTATCTAAGGACACCATGGAGAAATCAGAAAGTCTAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208763 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSVQKVPAIALCSGVSLALLHFLCLAACLNESPGQNSKDEKLCPENFTRILDSLLDGYDNRLRPGFGGP
  VTEVKTDIYVTSFGPVSDVEMEYTMDVFFRQTWIDKRLKYDGPIEILRLNNMMVTKVWTPDTFFRNGKKS
  VSHNMTAPNKLFRIMRNGTILYTMRLTISAECPMRLVDFPMDGHACPLKFGSYAYPKSEMIYTWTKGPEK
  SVEVPKESSSLVQYDLIGQTVSSETIKSITGEYIVMTVYFHLRRKMGYFMIQTYIPCIMTVILSQVSFWI
  NKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFTNIQMQK
  AKKKISKPPPEVPAAPVLKEKHTETSLQNTHANLNMRKRTNALVHSESDVKSRTEVGNHSSKTSAVQESS
  EATPKAHLASSPNPFSRANAAETMSAAARGLSSAASPSPHGTLRPASLGSASTRPAFGSRLGRIKTTVNT
  TGAAGNVSATPPPPAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010251
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010251.1, NM_010251.2, NP_034381.1
• RefSeq Size: 4123 bp
• RefSeq ORF: 1659 bp
• Locus ID: 14397
• MW: 60.9 kDa
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. [UniProtKB/Swiss-Prot Function]