Glud1 (NM_008133) Mouse Tagged ORF Clone

CAT#: MR208853

Glud1 (Myc-DDK-tagged) - Mouse glutamate dehydrogenase 1 (Glud1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glud1
• Synonyms: AI118167; Gdh-X; Glud; Gludl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208853 representing NM_008133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGCCGTCTGGGCGAAGCGCTGCTGCTGTCCCGCGCCGGGCCCGCTGCCCTGGGCTCTGCGGCTG
  CAGACTCAGCCGCGCTGCTGGGCTGGGCCCGCGGACAGCCCTCCGCCGCCCCGCAACCCGGGCTCACGCC
  GGTCGCCCGGCGCCACTACAGCGAAGCGGCCGCCGACCGCGAAGACGACCCCAACTTCTTCAAGATGGTG
  GAGGGCTTCTTCGACCGCGGCGCCAGCATCGTAGAGGACAAGCTGGTGGAAGACCTGAAGACCCGGGAGA
  GCGAGGAGCAGAAGCGGAACCGGGTGCGCGGCATCCTGAGGATCATCAAGCCCTGCAACCATGTGTTGAG
  CCTCTCCTTCCCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGC
  CAGCACCGCACGCCCTGCAAGGGAGGTATCCGTTACAGCACTGACGTGAGTGTGGATGAAGTAAAAGCAC
  TGGCTTCCTTAATGACATACAAGTGCGCTGTGGTCGATGTACCGTTTGGAGGTGCTAAAGCAGGCGTTAA
  GATCAACCCCAAGAACTATACAGATAATGAATTAGAAAAAATTACACGGAGGTTCACTATGGAGCTGGCC
  AAGAAGGGTTTTATTGGTCCTGGCATTGATGTGCCTGCCCCAGACATGAGCACGGGTGAGCGGGAGATGT
  CCTGGATCGCTGACACCTATGCCAGCACCATAGGGCACTATGATATCAATGCGCATGCCTGTGTTACTGG
  GAAACCCATCAGTCAAGGAGGCATCCACGGGCGCATCTCCGCTACTGGCCGGGGTGTCTTCCATGGAATT
  GAAAACTTCATCAATGAGGCTTCTTACATGAGCATTTTAGGAATGACACCAGGCTTTGGCGATAAGACAT
  TTGTTGTTCAGGGATTTGGTAATGTGGGCCTGCACTCTATGAGATATTTACATCGTTTTGGTGCTAAATG
  TGTTGGTGTTGGAGAGTCTGATGGGAGTATATGGAATCCGGATGGGATTGACCCAAAAGAACTGGAAGAC
  TTCAAGTTGCAACATGGATCAATTCTGGGCTTCCCCAAAGCCAAGGTCTATGAAGGAAGCATCTTGGAGG
  CTGACTGTGACATTCTGATTCCTGCCGCCAGCGAGAAGCAGTTGACCAAATCCAATGCACCCAGAGTCAA
  AGCCAAGATCATTGCTGAAGGTGCCAATGGGCCAACCACTCCAGAGGCTGATAAGATTTTCCTGGAAAGA
  AACATCATGGTTATTCCCGATCTCTACTTAAATGCTGGAGGAGTAACAGTGTCTTACTTTGAGTGGCTAA
  AGAATCTAAATCATGTCAGCTACGGCCGATTGACCTTCAAATATGAAAGGGACTCTAACTACCACTTGCT
  CATGTCTGTTCAAGAGAGTTTAGAGAGAAAGTTTGGAAAGCATGGTGGAACTATTCCTGTGGTCCCCACA
  GCAGAGTTCCAGGACAGGATATCGGGTGCATCTGAGAAAGACATTGTGCACTCTGGCTTGGCCTACACAA
  TGGAGAGATCTGCCAGGCAAATTATGCGCACAGCCATGAAGTATAACCTGGGATTGGACCTGAGAACAGC
  TGCCTATGTCAATGCTATCGAGAAAGTCTTCAAGGTGTACAATGAAGCTGGTGTGACCTTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208853 representing NM_008133
  Red=Cloning site Green=Tags(s)
  
  MYRRLGEALLLSRAGPAALGSAAADSAALLGWARGQPSAAPQPGLTPVARRHYSEAAADREDDPNFFKMV
  EGFFDRGASIVEDKLVEDLKTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHS
  QHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELA
  KKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGI
  ENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCVGVGESDGSIWNPDGIDPKELED
  FKLQHGSILGFPKAKVYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLER
  NIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPVVPT
  AEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008133
• ORF Size: 1674 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008133.1, NM_008133.2, NM_008133.3, NM_008133.4, NP_032159.1
• RefSeq Size: 3158
• RefSeq ORF: 1677
• Locus ID: 14661
• MW: 61.8 kDa