Asns (NM_012055) Mouse Tagged ORF Clone

CAT#: MR208883

Asns (Myc-DDK-tagged) - Mouse asparagine synthetase (Asns)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asns
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208883 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGGCATCTGGGCCCTCTTCGGCAGCGATGACTGCCTTTCCGTGCAGTGTCTGAGTGCGATGAAGA
  TCGCGCACAGGGGGCCAGATGCATTTCGCTTTGAGAATGTCAATGGATACACCAACTGCTGCTTTGGCTT
  TCACCGCTTGGCTGTGGTTGACCCGCTGTTTGGAATGCAGCCGATAAGAGTGAGGAAATACCCTTATTTG
  TGGCTCTGTTACAATGGTGAAATCTACAACCACAAGGCGCTACAGCAACGTTTTGAATTTGAATATCAGA
  CCAATGTGGATGGTGAGATTATCCTCCACCTCTATGACAAAGGAGGCATCGAGAAAACCATCTGTATGCT
  GGACGGGGTGTTTGCATTCATCTTACTGGACACTGCCAATAAGAAAGTATTTCTGGGCAGAGACACCTAT
  GGAGTCAGGCCCTTGTTTAAAGCCATGACAGAAGATGGGTTTCTGGCTGTGTGTTCAGAAGCTAAAGGCC
  TTGTTTCCTTAAAACACTCCACCACTCCCTTCTTAAAAGTGGAGCCCTTCCTTCCTGGACACTATGAAGT
  TTTGGATTTAAAACCAAATGGCAAAGTTGCGTCTGTGGAAATGGTCAAATACCATCACTGTACGGATGAA
  CCATTGCATGCCATCTATGACAGCGTGGAGAAACTCTTCCCAGGCTTTGACCTAGAGACCGTGAAGAACA
  ATCTGCGTATCCTTTTTGACAACGCTATCAAGAAACGCTTGATGACAGACCGGAGGATTGGCTGCCTTTT
  ATCAGGGGGCCTGGACTCGAGCTTGGTTGCTGCCTCTCTGCTGAAGCAACTCAAGGAGGCCCAAGTTCAG
  TATCCTCTCCAGACATTTGCTATTGGCATGGAGGACAGCCCCGATCTCCTGGCCGCTAGAAAGGTGGCAA
  ATTATATTGGAAGCGAGCATCATGAAGTCCTTTTTAACTCTGAAGAAGGCATTCAGGCCCTGGATGAAGT
  CATATTTTCCTTGGAAACTTATGATATTACGACAGTTCGGGCATCTGTGGGCATGTATTTAATTTCCAAG
  TATATTCGGAAGAACACAGACAGCGTGGTGATCTTCTCCGGAGAGGGGTCAGATGAACTTACACAGGGCT
  ATATATATTTCCACAAGGCTCCTTCCCCTGAGAAGGCCGAGGAGGAGAGTGAGAGACTGCTGAAGGAACT
  CTACCTGTTTGATGTTCTCCGGGCCGACCGCACTACTGCTGCACACGGTCTCGAACTGAGAGTCCCCTTT
  CTGGATCATCGGTTTTCTTCCTATTACCTGTCTCTGCCGCCAGATATGAGAATTCCAAAAAATGGCATAG
  AAAAACATCTCCTGAGAGAGACTTTTGAGGACTGCAACCTGCTACCCAAAGAGATTCTCTGGCGACCCAA
  AGAAGCCTTCAGTGATGGGATCACCTCAGTCAAGAACTCCTGGTTCAAGATTTTGCAGGACTATGTTGAA
  CATCAGGTTGATGATGAGATGATGTCTGCAGCCTCCCAGAAGTTTCCCTTCAATACTCCCAAAACTAAGG
  AAGGCTACTTCTACCGTCAGATCTTTGAACGCCATTACCCAGGCCGGGCTGATTGGCTGACTCATTATTG
  GATGCCTAAGTGGATCAATGCTACTGACCCTTCTGCCCGCACTCTGACCCATTATAAGTCAGCTGCCAAA
  GCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208883 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVRKYPYL
  WLCYNGEIYNHKALQQRFEFEYQTNVDGEIILHLYDKGGIEKTICMLDGVFAFILLDTANKKVFLGRDTY
  GVRPLFKAMTEDGFLAVCSEAKGLVSLKHSTTPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCTDE
  PLHAIYDSVEKLFPGFDLETVKNNLRILFDNAIKKRLMTDRRIGCLLSGGLDSSLVAASLLKQLKEAQVQ
  YPLQTFAIGMEDSPDLLAARKVANYIGSEHHEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISK
  YIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLKELYLFDVLRADRTTAAHGLELRVPF
  LDHRFSSYYLSLPPDMRIPKNGIEKHLLRETFEDCNLLPKEILWRPKEAFSDGITSVKNSWFKILQDYVE
  HQVDDEMMSAASQKFPFNTPKTKEGYFYRQIFERHYPGRADWLTHYWMPKWINATDPSARTLTHYKSAAK
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012055
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012055.1, NM_012055.2, NM_012055.3, NP_036185.1
• RefSeq Size: 1927 bp
• RefSeq ORF: 1686 bp
• Locus ID: 27053
• MW: 64.3 kDa