Add2 (BC053032) Mouse Tagged ORF Clone
CAT#: MR208912
- TrueORF®
Add2 (Myc-DDK-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200)
"BC053032" in other vectors (4)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Add2 |
Synonyms | 2900072M03Rik|add97 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208912 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAGGACACGGTTCCCGAGGCGGCCTCCCCGCCACCCTCTCAGGGGCAGCACTACTTTGACCGGT TCTCTGAAGATGATCCTGAGTACTTGCGACTTCGCAACCGTGCAGCTGACCTGCGACAGGACTTCAACTT GATGGAGCAGAAGAAGAGGGTCACCATGATCCTGCAGAGCCCTTCTTTCAGGGAGGAGCTGGAAGGCCTC ATCCAGGAGCAAATGAAGAAGGGCAACAACTCCTCCAACATCTGGGCCCTCCGACAGATCGCGGACTTCA TGGCCAGCACCTCCCACGCAGTCTTCCCAGCTTCTTCCATGAACTTCTCCATGATGACACCCATCAACGA CCTCCACACTGCCGACTCCCTGAACCTGGCCAAGGGGGAGAGGCTTATGCGGTGCAAGATCAGCAGCGTC TACCGTCTCCTGGACCTCTACGGCTGGGCGCAGCTCAGTGACACCTACGTCACGCTGAGAGTGAGCAAGG AGCAGGACCACTTCCTGATCAGCCCCAAGGGGGTTTCCTGCAGCGAGGTCACAGCCTCCAGCCTGATTAA AGTGAACATTCTGGGAGAGGTGGTGGAGAAGGGCAGCAGTTGCTTCCCGGTGGACACCACGGGCTTCAGT CTGCACTCAGCCATCTATGCCGCCAGGCCCGACGTGCGGTGTGCCATCCACCTGCACACGCCTGCCACCG CAGCGGTGTCAGCTATGAAGTGCGGCCTCCTGCCGGTCTCCCATAATGCCCTGCTGGTGGGGGACATGGC CTACTATGACTTCAATGGGGAAATGGAGCAGGAAGCTGACCGAATCAACTTGCAGAAGTGCCTTGGACCC ACCTGCAAGATTCTGGTGCTAAGAAACCATGGCATGGTCGCCCTGGGTGACACCGTGGAGGAAGCTTTCT ACAAGGTCTTCCATCTGCAGGCTGCGTGTGAGGTACAGGTGTCGGCTCTGTCCAGTGCTGGGGGCACTGA GAACCTCATCCTCTTGGAGCAAGAGAAACACCGGCCGCACGAGGTGGGCTCTGTGCAGTGGGCCGGCAGC ACCTTCGGGCCCATGCAGAAGAGCCGGCTGGGAGAGCATGAATTTGAAGCCCTCATGAGGATGCTGGACA ATTTAGGCTACAGAACAGGCTATACGTACCGCCACCCCTTTGTCCAAGAGAAAACCAAACACAAAAGTGA AGTGGAGATCCCAGCCACAGTCACTGCCTTTGTGTTTGAAGAGGATGGAGTCCCAGTCCCCGCCCTGCGC CAGCACGCCCAGAAGCAGCAGAAGGAAAAGACCCGCTGGCTTAACACTCCCAACACCTACCTGCGGGTGA ACGTGGCTGACGAGGTGCAGAGGAACATGGGCAGTCCCCGACCAAAGACCACGTGGATGAAGGCTGATGA AGTGGAAAAGTCCAGCAGCGGCATGCCCATACGGATTGAAAACCCAAACCAATTTGTGCCTCTCTACACT GACCCCCAGGAAGTTCTGGACATGAGGAACAAGATTCGAGAGCAAAACCGACAAGACATAAAGTCAGCCG GGCCTCAGTCTCAGCTCTTGGCCAGTGTCATCGCAGAGAAGAGCCGGAGTCCGGTACAGCAGAGACTGCC CCCAACCGAAGGGGAAGTGTATCAGACTCCTGGGGCTGGGCAGGGGACCCCTGAGTCCTCAGGCCCGCTC ACCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208912 protein sequence
Red=Cloning site Green=Tags(s) MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGL IQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSV YRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFS LHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGP TCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGS TFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALR QHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYT DPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEVYQTPGAGQGTPESSGPL TP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC053032 |
ORF Size | 1686 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | BC053032, AAH53032 |
RefSeq Size | 3431 |
RefSeq ORF | 1688 |
Locus ID | 11519 |
MW | 62.8 kDa |
Gene Summary | This gene encodes the beta subunit of the adducin family. Adducins, encoded by alpha, beta and gamma genes, are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein, primarily found in the brain and hematopoietic cells, is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments, bundles actin and caps barbed ends of actin filaments. In mouse, deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Dec 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218134 | Add2 (untagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200), (10ug) |
USD 730.00 |
|
MG208912 | Add2 (GFP-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
USD 370.00 |
|
MR208912L3 | Lenti ORF clone of Add2 (Myc-DDK-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
USD 540.00 |
|
MR208912L4 | Lenti ORF clone of Add2 (mGFP-tagged) - Mouse adducin 2 (beta) (cDNA clone MGC:62261 IMAGE:6401200) |
USD 540.00 |
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