Eme1 (NM_177752) Mouse Tagged ORF Clone

CAT#: MR208978

Eme1 (Myc-DDK-tagged) - Mouse essential meiotic endonuclease 1 homolog 1 (S. pombe) (Eme1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eme1
• Synonyms: 6820428D13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208978 representing NM_177752
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACAGTACAAAATCGAGAGAGTTTGGGGCTTCTCTCTTTTCCCGTGATGGTTGCTATGGTTTGTT
  GTGCACACAGCTCCAATGAACCCAGCAACATGTCATACGTGAAAGAGACAGTGGACCGACTGCTCAAAGG
  ATATGACATTCGCTTGCGGCCGGACTTTGGAGGGCCCCCGGTGGACGTCGGGATGCGGATCGATGTCGCC
  AGCATAGACATGGTCTCGGAAGTGAATATGGATTATACACTCACCATGTATTTCCAGCAGTCTTGGAAGG
  ACAAGAGGCTTTCATATTCTGGAATCCCATTAAATCTGACCCTTGACAACAGAGTAGCTGACCAACTCTG
  GGTACCAGACACCTACTTTCTCAATGACAAGAAATCATTTGTGCATGGGGTCACAGTGAAAAACCGAATG
  ATCAGGCTGCATCCTGATGGAACTGTTCTCTATGGACTACGGATCACAACCACTGCAGCATGCATGATGG
  ATCTTCGAAGATACCCTCTGGATGAGCAAAACTGCACCCTGGAGATTGAAAGTTATGGCTATACCACGGA
  TGACATCGAATTTTACTGGAATGGAGGAGAGGGAGCAGTAACTGGGGTCAATAAAATTGAGCTTCCCCAA
  TTTTCAATTGTTGACTACAAGATGGTGTCCAAGAAGGTGGAGTTCACAACAGGGGCATATCCACGACTAT
  CACTAAGTTTTCGTCTAAAGAGAAACATTGGTTACTTCATTTTGCAGACCTACATGCCTTCCACACTGAT
  TACAATCCTGTCCTGGGTGTCGTTTTGGATCAACTATGATGCATCTGCAGCCAGAGTCGCACTAGGAATC
  ACTACGGTGCTGACCATGACCACCATCAGCACTCACCTCAGGGAGACTTTGCCGAAGATCCCTTACGTCA
  AAGCGATTGACATCTATCTCATGGGCTGTTTTGTGTTTGTGTTCCTGGCTCTACTGGAATATGCTTTTGT
  CAATTACATTTTCTTCGGAAAAGGCCCTCAGAAAAAGGGAGCGAGCAAACAAGACCAGAGTGCCAATGAA
  AAGAACAGACTGGAGATGAACAAAGTCCAAGTTGATGCCCACGGCAATATTCTCCTCAGCACCCTGGAAA
  TCAGGAACGAGACCAGTGGCTCCGAGGTGCTCACAGGTGTAAGTGACCCCAAGGCCACCATGTACTCGTA
  CGACAGCGCCAGCATCCAGTACCGCAAGCCACTGAGCAGCCGCGAGGGCTTCGGGCGTGGGCTGGACAGG
  CACGGGGTACCGGGCAAGGGGCGCATCCGCAGGCGCGCCTCGCAGCTCAAAGTGAAGATCCCCGACTTGA
  CTGACGTGAACTCCATAGACAAGTGGTCCCGAATGTTCTTCCCCATCACCTTTTCTCTTTTTAATGTCGT
  TTATTGGCTTTACTATGTACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208978 representing NM_177752
  Red=Cloning site Green=Tags(s)
  
  MWTVQNRESLGLLSFPVMVAMVCCAHSSNEPSNMSYVKETVDRLLKGYDIRLRPDFGGPPVDVGMRIDVA
  SIDMVSEVNMDYTLTMYFQQSWKDKRLSYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRM
  IRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWNGGEGAVTGVNKIELPQ
  FSIVDYKMVSKKVEFTTGAYPRLSLSFRLKRNIGYFILQTYMPSTLITILSWVSFWINYDASAARVALGI
  TTVLTMTTISTHLRETLPKIPYVKAIDIYLMGCFVFVFLALLEYAFVNYIFFGKGPQKKGASKQDQSANE
  KNRLEMNKVQVDAHGNILLSTLEIRNETSGSEVLTGVSDPKATMYSYDSASIQYRKPLSSREGFGRGLDR
  HGVPGKGRIRRRASQLKVKIPDLTDVNSIDKWSRMFFPITFSLFNVVYWLYYVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177752
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 2228
• RefSeq ORF: 1713
• Locus ID: 268465
• MW: 63.9 kDa