Dpysl3 (NM_009468) Mouse Tagged ORF Clone

CAT#: MR208981

Dpysl3 (Myc-DDK-tagged) - Mouse dihydropyrimidinase-like 3 (Dpysl3), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dpysl3
• Synonyms: CRMP-4; DRP-3; TUC4; Ulip; ULIP-1; Ulip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208981 representing NM_009468
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAAATGGAAGTGTAGTGACCACGTTCTTCCTCTCAGGTCTTCCCCATCCACCAGTCCTGGACTCCA
  TGCTCTTTGGGATCTTCCTTGTGATTTATATTCTTACTGTGTTGGGAAACCTTCTCATCCTGATGGTGAT
  CAGGGTGGATTCCCACCTCCACACACCAATGTACTACTTCCTCACCAACCTCTCCTTTATTGACATGTGG
  TTCTCCACAGTCACAGTGCCTAAAATGCTGATGACTTTGGTGTCCACAGGGGGCGGGGCTATCTCCTTCC
  ATAGCTGTGTGGCACAGCTCTATTGCTTCCACTTCCTGGGTAGCACCGAGTGTTTCCTGTACACAGTCAT
  GTCCTATGATCGCTACCTGGCCATCAGTTACCCACTCAGGTACAGCAGCATGATGAGTGGGAGAGTGTGT
  GCCCTCCTGGCAGCAGGAACATGGATCACTGGTTCCCTGCACTCTGCTGTCCAGACTACACTGATTTTTC
  ATTTGCCCTACTGTGGACCCAATGAGATTCAGCATTATTTCTGTGATGGACCACCCATCCTCAAGCTGGC
  CTGTGCAGACACATCTGCCATCGAGATGGTCATCTTTGTGAACATTGGGGTAGTGGCTTCGGGCTGCTTT
  TTTCTGATTTCGCTGTCCTATGTGTCCATTGTCTGCTCCATCCTGAGGATTCGCACTTCAGAGGGCCGGC
  ACAGAGCTTTCCAAACATGTGCCTCACACTGCATCGTGGTCCTCTGTTTCTTTGTGCCCTGTGTTTTCAT
  CTACCTGAGGCCAGGCTCCAGGGATGCTGTGGATGGAGTGGTGACAGTTTTCTACACTGTGTTGACACCC
  CTACTCAACCCTGTTGTGTACACCCTGAGGAACAAAGAGGTGAAGAAAGCACTGTTTAAACTCAAAGATA
  AAGTAGCATTTTCTCAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208981 representing NM_009468
  Red=Cloning site Green=Tags(s)
  
  MLNGSVVTTFFLSGLPHPPVLDSMLFGIFLVIYILTVLGNLLILMVIRVDSHLHTPMYYFLTNLSFIDMW
  FSTVTVPKMLMTLVSTGGGAISFHSCVAQLYCFHFLGSTECFLYTVMSYDRYLAISYPLRYSSMMSGRVC
  ALLAAGTWITGSLHSAVQTTLIFHLPYCGPNEIQHYFCDGPPILKLACADTSAIEMVIFVNIGVVASGCF
  FLISLSYVSIVCSILRIRTSEGRHRAFQTCASHCIVVLCFFVPCVFIYLRPGSRDAVDGVVTVFYTVLTP
  LLNPVVYTLRNKEVKKALFKLKDKVAFSQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009468
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2076 bp
• RefSeq ORF: 1713 bp
• Locus ID: 22240
• MW: 62.4 kDa
• Gene Summary: This gene encodes a protein that belongs to the TUC (TOAD-64/Ulip/CRMP) family of proteins. Members of this family are phosphoproteins that function in axonal guidance and neuronal differentiation during development and regeneration of the nervous system. A mutation in the human gene is associated with amyotrophic lateral sclerosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]