Trim23 (BC059017) Mouse Tagged ORF Clone

CAT#: MR209027

Trim23 (Myc-DDK-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim23
• Synonyms: 6330516O20Rik|AI450195|Arfd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209027 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCTCGCTGTAAACAAGCCCGGAGCCGGAGTAGACAGTGGGCGGCAGGGCAGCCGGGGGACGG
  CTGTGGTGAAGGTTCTGGAGTGTGGGGTTTGTGAAGATGTCTTCTCTTTGCAAGGAGATAAAGTTCCTCG
  CCTCCTCCTCTGTGGCCACACAGTCTGTCATGACTGTCTTACTCGCTTGCCTCTGCATGGAAGAGCAATA
  CGTTGCCCATTTGATCGACAAGTAACAGACCTAGGAGACTCAGGTGTATGGGGTTTGAAAAAGAACTTTG
  CTTTACTGGAGCTTTTAGAACGTTTGCAAAATGGGCACATTGGTCAGTATGGAGCTGCAGAAGAGGCCAT
  TGGGACATCTGGAGAGAGCATCATTCGCTGTGATGAAGATGAAGCTCACGTTGCATCTGTATATTGCACT
  GTGTGTGCAACTCACTTGTGCTCAGACTGTTCTCAAGTTACTCATTCTACAAAGACATTAGCAAAGCATA
  GGCGTGTGCCTCTAGCTGATAAACCTCATGAGAAAACCATGTGCTGTCAGCACCAGGTGCATGCCATTGA
  GTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGTCCACTCATGTGCTGTGTCTGCAAAGAATATGGAAAG
  CACCAAGGTCACAAGCATTCAGTACTGGAACCAGAAGCTAATCAGATCCGAGCATCAATTTTAGACATGG
  CTCACTGCATACGGACCTTCACTGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGGATTGTTCAGCA
  CATTGAAGGAGGAGAACAAATAGTAGAAGATGGAATTGGAATGGCTCACACAGAACATGTCCCAGGTACT
  GCAGAGAATGCCCGATCATGTGTCAGAGCTTATTTTTCTGATTTACATGAAACTCTTTGTCGTCAAGAAG
  AAATGGCTCTAAGTGTTGTTGATGCCCATGTTCGAGAAAAACTGATTTGGCTTAGGCAGCAACAAGAGGA
  TATGACTATTCTCTTGTCCCAGGTCTCAACAGCCTGTCTCCACTGTGAAAAAACTCTGCAGCAGGATGAT
  TGCAGAGTTGTCTTGGCAAAACAAGAAATCACAAGATTACTAGAAACACTGCAGAAACAGCAGCAGCAGT
  TTACAGAGGTTGCAGATCATATTCAGTTGGATGCCAGTATTCCAGTCACTTTTACAAAGGACAATAGAGT
  TCATATTGGACCCAAAATGGAAATCCGAGTAGTCACATTAGGATTAGATGGTGCTGGAAAAACTACCATT
  TTGTTCAAGTTAAAACAAGATGAATTTATGCAACCTATTCCAACAATTGGTTTTAATGTGGAAACTGTGG
  AATACAAAAATCTAAAATTCACCATTTGGGATGTGGGTGGAAAACACAAATTAAGACCATTGTGGAAACA
  TTATTACCTCAATACACAAGCTGTTGTATTTGTTGTTGATAGTAGTCATAGAGACAGAATTAGTGAAGCA
  CACAGTGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCCTTACTCTTGATTTTTGCTAACA
  AACAGGATGTAGCTGGAGCACTTTCAGTAGAAGAAATCACTGAACTTCTCAGTCTCCATAAACTATGCTG
  TGGAAGGAGTTGGTATATTCAGGGCTGTGATGCTCGAAGTGGAATGGGGCTCTACGAAGGGTTGGACTGG
  CTGTCCCGGCAACTTGTGGCTGCTGGAGTGTTGGATGTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209027 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALAVNKPGAGVDSGRQGSRGTAVVKVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLTRLPLHGRAI
  RCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGHIGQYGAAEEAIGTSGESIIRCDEDEAHVASVYCT
  VCATHLCSDCSQVTHSTKTLAKHRRVPLADKPHEKTMCCQHQVHAIEFVCLEEGCQTSPLMCCVCKEYGK
  HQGHKHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGT
  AENARSCVRAYFSDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSQVSTACLHCEKTLQQDD
  CRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTI
  LFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEA
  HSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDW
  LSRQLVAAGVLDVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059017
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059017, AAH59017
• RefSeq Size: 3385 bp
• RefSeq ORF: 1724 bp
• Locus ID: 81003
• MW: 63.9 kDa