C8b (NM_133882) Mouse Tagged ORF Clone

CAT#: MR209183

C8b (Myc-DDK-tagged) - Mouse complement component 8, beta polypeptide (C8b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8b
• Synonyms: 4930439B20Rik; AI595927
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209183 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATAGGGGCTCAGGTTTGGAGGGCACTGGCAAAGTCGTGTCTCCTCTGTGCTACTCTGGGATGTC
  TCCATTTCCCTGGTTCCAGAGGTGGGAAGCCCGATTTCTTTGAGACAAAGGCAGTCAATGGGAGCCTTGT
  CAAGAGCAGACCAGTACGGAGTGTGGCGGAAGCCCCAGCACCTATTGACTGTGAGCTATCCACCTGGTCT
  TCCTGGACTGCATGTGACCCCTGTCAGAAGAAAAGGTACAGACATACTTACTTGCTCCGGCCCTCTCAGT
  TCTATGGGGAATTGTGTGACTTGTCCGACAAGGAAGTTGAAGACTGTGTTACCAACCAACCCTGCAGGAG
  TCAAGTACGATGTGAAGGCTTTGTGTGTGCACAGACAGGGAGGTGTGTGAATCGCAGACTCCTTTGCAAT
  GGGGACAATGACTGTGGAGACCAATCAGATGAAGCCAACTGTAGGAGGATTTATAAAAACTGTCAACGGG
  AGATGGAGCAGTACTGGGCAATTGACAGGCTGGCCAGTGGGATAAATCTGTTCACAAACACTTTTGAGGG
  CCCAGTTCTCGATCACCGGTATTATGCTGGTGGATGCTCCCCCCACTACATCCTGGACACCAACTTTAGG
  AAGCCATACAACGTAGAAAGCTACACCCCACAGACCAAATGTGAATATGAATTCACACTTACAGAATATG
  AATCATATTCAGATTTTGAACGTCTAGTGATAGAAAAGAAAACCCACATGTTTAATTTCACGTCTGGTTT
  TAAAGTGGATGGCGTTATGGATCTTGGAATTAAAGTAGAAAGCAACGAAGGCAAAAATTATGTGACCAGA
  ACCAAACGCTTCGCCCACACTCAAAGCAAGTTCCTCCACGCGCGCTCTGTCCTTGAAGTGGCACATTACA
  AGCTGAAGTCTAGAAGCCTCATGCTCCATTATGAATTCCTTCAGAGGGTCAAGAGCCTGCCCCTGGAGTA
  TAGCTATGGGGAATACCGAGATCTTCTTCGTGACTTCGGGACCCATTTCATCACTGAAGCCGTACTTGGG
  GGCATCTACGAGTACACGCTTATCATGAACAAAGACGCCATGGAGCAAGGAGACTACACTCTTAGCCATG
  TTACTGCCTGTGCTGGAGGAAGTTTCGGTATTGGTGGTATGGTCTATAAAGTCTATGTCAAAGTGGGCGT
  TTCCGCAAAGAAATGCAGTGACATTATGAAAGAAATCAACGAAAGAAATAAGAGGAGCACCATGGTAGAA
  GACTTGGTAGTTCTTGTGAGAGGAGGGACAAGTGAGGATATCACTGCCTTGGCATACAAGGAGCTACCAA
  CCCCTGAACTGATGGAGGCATGGGGCGATGCTGTGAAGTACAACCCAGCAATCATTAAGATCAAGGCTGA
  GCCCCTGTATGAGCTTGTGACAGCCACAGACTTTGCATACTCCAGCACAGTGAAGCAGAACTTAAAGAAG
  GCACTAGAAGAATTCCAGAGTGAGGTCAGCTCCTGCCGCTGTGCTCCTTGCAGAGGGAATGGAGTCCCTG
  TCCTGAAAGGATCCCGCTGTGAGTGCATCTGCCCCGGTGGCTTCCAAGGCACAGCCTGTGAGGTTACCTA
  TCGGAAAGACATCCCCATAGATGGGAAGTGGAGTTGCTGGTCTGACTGGTCTGCATGCTCTGGAGGACAC
  AAAACAAGACACAGGCAGTGCAACAATCCAGCCCCTCACAAAGGAGGCAGCCCCTGTTCGGGTCCTGCTT
  CCGAAACACTCAACTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209183 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKIGAQVWRALAKSCLLCATLGCLHFPGSRGGKPDFFETKAVNGSLVKSRPVRSVAEAPAPIDCELSTWS
  SWTACDPCQKKRYRHTYLLRPSQFYGELCDLSDKEVEDCVTNQPCRSQVRCEGFVCAQTGRCVNRRLLCN
  GDNDCGDQSDEANCRRIYKNCQREMEQYWAIDRLASGINLFTNTFEGPVLDHRYYAGGCSPHYILDTNFR
  KPYNVESYTPQTKCEYEFTLTEYESYSDFERLVIEKKTHMFNFTSGFKVDGVMDLGIKVESNEGKNYVTR
  TKRFAHTQSKFLHARSVLEVAHYKLKSRSLMLHYEFLQRVKSLPLEYSYGEYRDLLRDFGTHFITEAVLG
  GIYEYTLIMNKDAMEQGDYTLSHVTACAGGSFGIGGMVYKVYVKVGVSAKKCSDIMKEINERNKRSTMVE
  DLVVLVRGGTSEDITALAYKELPTPELMEAWGDAVKYNPAIIKIKAEPLYELVTATDFAYSSTVKQNLKK
  ALEEFQSEVSSCRCAPCRGNGVPVLKGSRCECICPGGFQGTACEVTYRKDIPIDGKWSCWSDWSACSGGH
  KTRHRQCNNPAPHKGGSPCSGPASETLNC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133882
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133882.1, NM_133882.2, NP_598643.1
• RefSeq Size: 2239
• RefSeq ORF: 1770
• Locus ID: 110382
• MW: 66.2 kDa
• Gene Summary: This gene encodes the beta subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the beta subunit, which associates with the alpha and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the alpha subunit. [provided by RefSeq, Oct 2015]