Cbfa2t2 (NM_009823) Mouse Tagged ORF Clone

CAT#: MR209247

Cbfa2t2 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) (Cbfa2t2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbfa2t2
• Synonyms: A430091M07; C330013D05Rik; Cbfa2t2h; MTGR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209247 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGCGTCCCTGGAGCGGCCGCCTTCCAACTTGGTTGTGAGAAAAGGGTGCCAGCAATGCCTGGAT
  CGCCTGTGGAAGTGAAGATACAGTCAAGATCCTCACCTCCCATCATGCCACCCCTCCCACCAATAAATCC
  TGGAGGACCGAGGCCAGTGTCCTTTACTCCTACAGCATTAAGCAATGGCATCAACCATTCCCCTCCTACC
  CTGAATGGTGCCCCATCGCCACCACAGAGATTCAGCAATGGGCCTGCCTCCTCCACATCGTCAGCACTAA
  CGAATCAACAGCTGCCGGCTACTTGTGGTGCTCGGCAGCTCAGCAAGTTGAAACGCTTCCTCACCACTCT
  GCAGCAGTTTGGCAATGATATCTCACCTGAGATCGGCGAGAAGGTTCGGACTCTCGTTCTAGCCTTGGTG
  AACTCAACAGTAACAATTGAAGAATTCCATTGCAAGCTCCAGGAGGCCACAAACTTTCCTCTTCGTCCAT
  TTGTGATTCCATTTCTCAAGGCCAACCTGCCCCTGCTGCAGCGAGAACTGCTGCACTGTGCCCGGGCTGC
  CAAGCAGACCCCGTCCCAGTACCTGGCCCAGCATGAGCACCTTCTGCTCAACACCAGTATCGCATCTCCT
  GCTGACTCTTCTGAACTGCTCATGGAGGTTCATGGAAATGGAAAGAGGCCCAGTCCGGAAAGGAGGGACG
  AGAATAATTTTGAAAGAGATACAGTTCCTCCTGAACCGCCTGCCAAGAGAGTGTGTACTATCAGCCCTGC
  TCCTCGGCACAGTCCCGCCCTCACTGTTCCCCTTATGAATCCTGGGGGCCAGTTCCATCCTACTCCACCA
  CCTCTTCAGCACTATACACTAGAAGACATTGCAACTTCTCACCTGTATCGGGAACCAAACAAGATGCTAG
  AACACCGAGAAGTTCGTGAGAGGCACCACAATCTTAGTCTCAATGGAGGCTATCAAGATGAGTTGGTAGA
  CCACCGTTTGACAGAAAGGGAGTGGGCTGATGAATGGAAACACCTTGACCATGCACTGAATTGCATTATG
  GAAATGGTAGAGAAAACTAGGCGCTCCATGGCAGTTCTGAGGCGCTGTCAGGAATCTGACCGTGAAGAAC
  TCAACTATTGGAAACGGCGGTTTAATGAAAATACAGAGTTGAGGAAAACTGGGACTGAGTTGGTCTCTAG
  GCAGCACAGCCCTGGGAGTACAGATTCTCTCAGCAACGATTCTCAGCGGGAATTTACCAGTAGACCAGCA
  ACAGGATATGTGCCTGTGGAGTTTTGGAAGAAAACAGAAGAAGCCGTGAATAAGGTGAAAATTCAGGCCA
  TGTCAGAGGTACAGAAGGCTGTAGCTGAGGCAGAGCAAAAAGCCTTTGAAGTGATTGCAACGGAGAGAGC
  TCGAATGGAGCAGACCATAGCGGATGTCAAACGGCAGGCTGCAGAAGATGCTTTCCTGGTCATCAATGAG
  CAAGAAGAGTCCACAGAGAACTGCTGGAACTGTGGCCGAAAAGCCAGTGAGACGTGCAGTGGCTGCAACA
  TTGCTCGCTACTGCGGCTCCTTCTGCCAGCATAAGGACTGGGAGCGGCACCACCGCCTCTGTGGCCAGAG
  TCTACATGGCCACAGTCCCCACAGCCAGAGCAGGCCACTGCTCCCTGGAGGGCGGGGCTCAGCCAGGTCT
  GCTGACTGCAGTGTGCCCAGCCCAGCCCTGGACAAGACCTCAGCTACCACCTCACGGTCCTCAACACCTG
  CCTCTGTGACAGCCATTGATGCCAACGGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209247 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVGVPGAAAFQLGCEKRVPAMPGSPVEVKIQSRSSPPIMPPLPPINPGGPRPVSFTPTALSNGINHSPPT
  LNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFGNDISPEIGEKVRTLVLALV
  NSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTPSQYLAQHEHLLLNTSIASP
  ADSSELLMEVHGNGKRPSPERRDENNFERDTVPPEPPAKRVCTISPAPRHSPALTVPLMNPGGQFHPTPP
  PLQHYTLEDIATSHLYREPNKMLEHREVRERHHNLSLNGGYQDELVDHRLTEREWADEWKHLDHALNCIM
  EMVEKTRRSMAVLRRCQESDREELNYWKRRFNENTELRKTGTELVSRQHSPGSTDSLSNDSQREFTSRPA
  TGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQTIADVKRQAAEDAFLVINE
  QEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQSLHGHSPHSQSRPLLPGGRGSARS
  ADCSVPSPALDKTSATTSRSSTPASVTAIDANGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009823
• ORF Size: 1785 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009823.1, NP_033953.1
• RefSeq Size: 6097 bp
• RefSeq ORF: 1782 bp
• Locus ID: 12396
• MW: 65.9 kDa