Ptgs1 (NM_008969) Mouse Tagged ORF Clone

CAT#: MR209322

Ptgs1 (Myc-DDK-tagged) - Mouse prostaglandin-endoperoxide synthase 1 (Ptgs1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptgs1
• Synonyms: Cox-1; Cox-3; COX1; PGHS-1; Pghs1; PHS 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209322 representing NM_008969
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCGAAGGAGTCTCTCGCTCTGGTTTCCCCTGCTGCTGCTCCTGCTGCTGCCGCCGACACCCTCGG
  TCCTGCTCGCAGATCCTGGGGTGCCCTCACCAGTCAATCCCTGTTGTTACTATCCGTGCCAGAACCAGGG
  TGTCTGTGTCCGCTTTGGCCTCGACAACTACCAGTGTGATTGTACTCGCACGGGCTACTCAGGCCCCAAC
  TGTACCATCCCTGAGATCTGGACCTGGCTTCGGAATTCTCTGCGGCCCAGCCCCTCGTTCACCCATTTCC
  TGCTGACACATGGATACTGGCTCTGGGAATTTGTGAATGCCACCTTCATCCGAGAAGTACTCATGCGCCT
  GGTACTCACAGTGCGGTCCAACCTTATCCCCAGCCCTCCGACCTACAACTCAGCGCATGACTACATCAGC
  TGGGAGTCCTTCTCCAATGTGAGCTACTATACTCGCATTCTGCCCTCTGTACCCAAAGACTGCCCCACAC
  CCATGGGGACCAAAGGGAAGAAACAGTTACCAGATGTTCAGCTTCTGGCCCAACAGCTGCTGCTGAGAAG
  GGAGTTCATTCCTGCCCCCCAGGGCACCAACATCCTGTTTGCCTTCTTTGCACAACACTTCACCCACCAG
  TTCTTCAAGACCTCTGGAAAGATGGGTCCTGGCTTTACCAAGGCCTTAGGCCACGGGGTAGACCTTGGCC
  ACATTTATGGAGATAATCTGGAACGACAGTATCACCTGCGGCTCTTCAAGGATGGGAAACTTAAGTACCA
  GGTGCTGGACGGAGAGGTGTACCCACCTTCCGTGGAACAGGCGTCCGTGTTGATGCGCTACCCACCAGGT
  GTCCCGCCTGAAAGGCAGATGGCTGTGGGCCAGGAGGTGTTTGGGTTGCTTCCGGGGCTGATGCTCTTCT
  CCACGATCTGGCTTCGTGAACATAACCGCGTGTGCGACCTGCTGAAGGAGGAGCATCCCACGTGGGATGA
  TGAGCAGCTCTTCCAGACCACTCGCCTCATCCTTATAGGAGAAACCATCAAAATTGTCATTGAGGAGTAT
  GTGCAGCACTTGAGTGGCTATTTCCTGCAGCTCAAGTTTGACCCGGAGCTGCTGTTCCGAGCCCAGTTCC
  AATATCGAAACCGCATCGCCATGGAATTTAACCATCTCTATCACTGGCATCCACTCATGCCCAACTCCTT
  CCAAGTGGGCTCACAAGAGTACAGCTACGAGCAGTTTTTATTTAACACTTCTATGCTGGTGGACTATGGG
  GTTGAGGCACTGGTGGATGCCTTCTCTCGCCAGAGGGCTGGCCGGATTGGTGGAGGTAGGAACTTTGACT
  ATCATGTTCTGCATGTGGCTGTGGATGTCATCAAGGAGTCCCGAGAGATGCGCCTACAGCCCTTCAATGA
  ATACCGAAAGAGGTTTGGCTTGAAGCCTTACACCTCTTTCCAGGAGCTCACAGGAGAGAAGGAGATGGCT
  GCTGAGTTGGAGGAGCTGTACGGTGACATCGATGCTTTAGAGTTCTACCCGGGGTTGCTGCTGGAGAAGT
  GCCAGCCCAACTCCATCTTTGGAGAAAGTATGATAGAGATGGGGGCTCCCTTTTCCCTCAAGGGCCTCCT
  AGGGAATCCCATCTGTTCCCCAGAGTACTGGAAACCCAGCACGTTCGGTGGTGACGTGGGCTTCAACCTT
  GTCAACACAGCCTCACTGAAGAAACTGGTCTGCCTCAACACCAAGACCTGCCCCTATGTTTCCTTCCGTG
  TGCCAGATTACCCTGGAGATGACGGGTCTGTCTTAGTGAGACGCTCCACTGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209322 representing NM_008969
  Red=Cloning site Green=Tags(s)
  
  MSRRSLSLWFPLLLLLLLPPTPSVLLADPGVPSPVNPCCYYPCQNQGVCVRFGLDNYQCDCTRTGYSGPN
  CTIPEIWTWLRNSLRPSPSFTHFLLTHGYWLWEFVNATFIREVLMRLVLTVRSNLIPSPPTYNSAHDYIS
  WESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDVQLLAQQLLLRREFIPAPQGTNILFAFFAQHFTHQ
  FFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYHLRLFKDGKLKYQVLDGEVYPPSVEQASVLMRYPPG
  VPPERQMAVGQEVFGLLPGLMLFSTIWLREHNRVCDLLKEEHPTWDDEQLFQTTRLILIGETIKIVIEEY
  VQHLSGYFLQLKFDPELLFRAQFQYRNRIAMEFNHLYHWHPLMPNSFQVGSQEYSYEQFLFNTSMLVDYG
  VEALVDAFSRQRAGRIGGGRNFDYHVLHVAVDVIKESREMRLQPFNEYRKRFGLKPYTSFQELTGEKEMA
  AELEELYGDIDALEFYPGLLLEKCQPNSIFGESMIEMGAPFSLKGLLGNPICSPEYWKPSTFGGDVGFNL
  VNTASLKKLVCLNTKTCPYVSFRVPDYPGDDGSVLVRRSTEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008969
• ORF Size: 1806 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008969.1, NM_008969.2, NM_008969.3, NM_008969.4, NP_032995.1
• RefSeq Size: 2864
• RefSeq ORF: 1809
• Locus ID: 19224
• MW: 69.5 kDa
• Gene Summary: This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. [provided by RefSeq, Jan 2014]