Ppp4r1 (NM_146081) Mouse Tagged ORF Clone

CAT#: MR209326

Ppp4r1 (Myc-DDK-tagged) - Mouse protein phosphatase 4, regulatory subunit 1 (Ppp4r1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp4r1
• Synonyms: 3110001J10Rik; Pp4r1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209326 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGACAATGATGTCGTAGAAGAAGAGCATCGCAGGCCAGAGGACGCTCCTTCAGACCTCAGTGCCC
  CTCACTCCAGCGCCAGGCTGGAGAGCCTCGAAGGCTGTGCTGCCAAGACACCTGGGCACTCTGCAGGTGA
  CGTGCCTGCTCCAGTGGACAGCTCCTTACTCTGTACTTTGTCCTCAGAGTCTCCTCAGGAAGCAGCTAGC
  AATGATGAGAATGGTAGAAAGCCTGACACCAACAGCAAGTCTGCGTCCCGGCCAGACGCTGGCACCAGCT
  CCCCAGAGGCCACTCCCTTAGACCAGGACATGTTCAACTCCTTCCATTTCTGGAGGACTCCTCTACCTAA
  AATAGATCTTGATAAAGAGCTCCAGCAGGACCCCGAGGAGAGACTCAGCCCAGAGAGAACAGGAGATGTA
  CCTGCAGCCCCTCTACCAGGCCCTCCCAATATCACCATGGCTACCCGGAAGGAACTAGAAGAAATGATAG
  AAAACCTTGAGCCACACATGGATGACCCGGATGTGAAAGCACAGGTGGACGTGCTGTCGGCTGCCCTGCG
  AGCTTCCAGCCTGGATGCTCACGAAGAGACTGGTGGTGTAGAGCAGCGGAGTGAGCTACAAGATGAAGTG
  TGTGTCAGTGAGCTTCCAGACTGTAACATAAGTCACGACACTTGTGTGCCTTTGATCAGCGCTGCTGAGG
  AGAATGCGGAGGCCACTCCTGATTATGTCCATGGAGGTGCAGACGTAAGCCCTGGTGATGGCTTCAGCCC
  GGATGAAGATAGGAGACCCAAAGTCCAGGATGTTGTACCACAAGCGTTACTGGATCAGTACTTGTCAATG
  ACCGACCCTTCTCGAGCACAGACAGTTGACACTGAGATCGCTAAGCACTGTGCATACAGTCTGCCGGGTG
  TGGCCCTGACTCTTGGCAGACAGAACTGGCACTGCTTGAGGGAGACTTACGAGACCCTAGCGTCGGACAT
  GCAGTGGAAAGTTCGAAGGACTCTAGCCTTCTCCATCCATGAGCTTGCAGTGATCCTTGGGGACCAGCTG
  ACAGCTGCAGACCTGGTTCCAATCTTCAATGGATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTC
  TTAAACACTTGCATGACTTTCTGAAGCTTCTTCACATTGATAAAAGAAGAGAGTACCTTTATCAGCTCCA
  GGAGTTTTTGGTGACAGACAACAGTAGAAATTGGCGCTTTCGAGCTGAACTGGCAGAACAGCTGATTTTA
  CTTCTGGAGTTATACAGTCCCAGAGATGTTTATGATTACCTACGACCCATCGCTCTGAATCTGTGTGCAG
  ACAAAGTTTCTTCAGTCCGCTGGATTTCCTACAAGTTGGTCAGTGAAATGGTGAAGAAACTCCACACAGC
  AACACCACCAACCTTCGGAGTAGATCTCATCAATGAGCTGGTAGAGAATTTTGGCAGGTGTCCAAAATGG
  TCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAAGACGACTGCCTCCCCATGGACCAGT
  TTGCTGTGCACTTGATGCCACATTTGCTGACCTTGGCAAATGACAGGGTTCCCAATGTTAGAGTGCTGCT
  TGCGAAAACCCTGCGACAGACTCTGCTGGAGAAAGAGTACTTCTTAGCCTCTGCCAGCTGTCACCAGGAG
  GCCGTGGAGCAGACAATCATGGCCCTGCAGATGGACCGAGACAGTGATGTCAAGTACTTTGCAAGCATTC
  ACCCCTCCAGTACCAAACTCTCTGAAGATGCAATGAGCACAGCTTCCTCCACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209326 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRDNDVVEEEHRRPEDAPSDLSAPHSSARLESLEGCAAKTPGHSAGDVPAPVDSSLLCTLSSESPQEAAS
  NDENGRKPDTNSKSASRPDAGTSSPEATPLDQDMFNSFHFWRTPLPKIDLDKELQQDPEERLSPERTGDV
  PAAPLPGPPNITMATRKELEEMIENLEPHMDDPDVKAQVDVLSAALRASSLDAHEETGGVEQRSELQDEV
  CVSELPDCNISHDTCVPLISAAEENAEATPDYVHGGADVSPGDGFSPDEDRRPKVQDVVPQALLDQYLSM
  TDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQL
  TAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLIL
  LLELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHTATPPTFGVDLINELVENFGRCPKW
  SGRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKEYFLASASCHQE
  AVEQTIMALQMDRDSDVKYFASIHPSSTKLSEDAMSTASSTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146081
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146081.1, NP_666193.1
• RefSeq Size: 3864 bp
• RefSeq ORF: 2856 bp
• Locus ID: 70351
• MW: 67.2 kDa