Pomt2 (BC052045) Mouse Tagged ORF Clone

CAT#: MR209338

Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pomt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209338 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGGCCATAGGCGGTGGCCTAGCGGGCTCCGAGCTGCGTCCCCGTAGGGGCCGCTGTGTGCCGC
  AGGCTGCCAGAGCCGTGAGCCGAGACGTGGTCCCCCAGGCTGCAGCACGGAAACTTAAACGGCCTGCTTG
  GAGCTCGCGGCGCTTCCAGGCGGCAGGGTGGTGGGCCACGTTGGCTGTGGTGACGCTGCTGTCCTTTGCC
  ACCCGCTTTCACCGGCTGGACCAGCCTGCTCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAA
  GTTACTATATTAACCGCACCTTTTTCTTTGATGTGCATCCACCACTGGGAAAGATGCTGATCGGTCTTGC
  TGGCTACCTGAGCGGGTATGATGGTACCTTTCTGTTCCAGAAGCCTGGGGACAGATACGAGCACCACAGC
  TACATGGGAATGAGAGGATTCTGTGCTTTCCTGGGTTCCTGGCTGATCCCCTTTGCCTACCTCACCGTAC
  TGGATCTGTCCAAGTCCTTCCCAGCAGCACTGCTCACCGCTGCCCTTCTCACCTTTGACACAGGATGCCT
  CACTCTATCCCAGTACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCGGCCATGCTGAGCATG
  GTCAAGTACAACTCCTGTGCTAACAGGCCTTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCA
  TTAGTCTCGCTGGTGCTTTAGGGGTCAAATTTGTTGGCCTCTTTATCATCGTGCAAGTGGGGTTGAACAC
  TATTTCAGACCTCTGGCATCTGTTTGGAGACCTCAGCCTTTCACTGGTGACTGTGGGGAAGCACCTAACT
  GCTCGCATCCTGTGCCTCATAGTGCTGCCTTTGGTGCTCTATGTGACCATTTTTGCTGTTCATGTCATGG
  TGCTGAATAAAAGTGGTCCTGGTGATGGCTTCTTCAGTTCTGCCTTTCAAGCCCGGCTTTCAGGGAACAG
  CCTTCACAATGCTTCTATCCCTGAACACCTGGCGTATGGCTCTGTAATCACTGTGAAGAATCTCCGGATG
  GCCATTGGCTACCTGCACTCTCATAGGCACCTCTACCCTGAGGGCATTGGTGCTCGCCAGCAGCAGGTTA
  CCACCTATTTGCATAAGGACTACAACAACTTGTGGATTATCAAGAAGTATAATGCCAACACAGATCCCCT
  AGACCCTTCATTCCCAGTGGAGTTTGTCAGACATGGAGACATCATACGACTAGAACACAAAGAAACTACT
  CGGAACCTGCACAGTCACTATCATGAGGCTCCCCTGACCCGGAAGCACTATCAGGTCACTGGCTATGGCA
  TAAATGGGACAGGGGACTCAAATGACTTCTGGCGGATTGAAGTTGTAAATAGAAAATTTGGGAACCGGAT
  CAAGGTACTGAGAAGTCGAATTCGCCTCATCCATTTGGTCACAGGTTGTGTCCTGGGATCGTCAGGAAAG
  ATTCTGCCTAAGTGGGGCTGGGAGCAGTTGGAAGTTACCTGTACTCCGTACCTAAAAGAAACCACTAACT
  CCATTTGGAACATAGAAGAGCATATCAATCCCAAATTGCCAAACATCAGCCTGGATGTGCTGCAGCCCAG
  TTTTCCTGAGATCTTGCTGGAGTCTCACATGGTCATGATCCGGGGAAATAATGGCCTCAAACCCAAGGAC
  AATGAGTTCACATCCAAGCCCTGGCACTGGCCTATCAATTATCAGGGCCTGCGCTTCTCAGGGGCCAATG
  ACACGGACTTCCGAGTCTATCTGCTCGGCAACCCTGTGAGTATCCCAGCTCTGAGTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209338 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPPAIGGGLAGSELRPRRGRCVPQAARAVSRDVVPQAAARKLKRPAWSSRRFQAAGWWATLAVVTLLSFA
  TRFHRLDQPAHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDRYEHHS
  YMGMRGFCAFLGSWLIPFAYLTVLDLSKSFPAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSM
  VKYNSCANRPFSAPWWFWLSLTGISLAGALGVKFVGLFIIVQVGLNTISDLWHLFGDLSLSLVTVGKHLT
  ARILCLIVLPLVLYVTIFAVHVMVLNKSGPGDGFFSSAFQARLSGNSLHNASIPEHLAYGSVITVKNLRM
  AIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKYNANTDPLDPSFPVEFVRHGDIIRLEHKETT
  RNLHSHYHEAPLTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRLIHLVTGCVLGSSGK
  ILPKWGWEQLEVTCTPYLKETTNSIWNIEEHINPKLPNISLDVLQPSFPEILLESHMVMIRGNNGLKPKD
  NEFTSKPWHWPINYQGLRFSGANDTDFRVYLLGNPVSIPALSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052045
• ORF Size: 1809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052045, AAH52045
• RefSeq Size: 2800 bp
• RefSeq ORF: 1811 bp
• Locus ID: 217734
• MW: 67.9 kDa
• Gene Summary: This gene encodes an integral membrane protein that belongs to the dolichyl-phosphate-mannose-protein mannosyltransferase family. The encoded enzyme is found in the membrane of the endoplasmic reticulum. This protein is a component of the protein O-mannosyltransferase enzyme complex which is involved in modification of the protein alpha-dystroglycan. Mutations in the human gene are a cause of different forms of muscular dystrophy-dystroglycanopathy (MDDG), type A2 (also known as Walker-Warburg syndrome), type B2 and type C2 (also known as limb-girdle muscular dystrophy). [provided by RefSeq, Sep 2015]