Araf (NM_009703) Mouse Tagged ORF Clone

CAT#: MR209341

Araf (Myc-DDK-tagged) - Mouse v-raf murine sarcoma 3611 viral oncogene homolog (Araf), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Araf
• Synonyms: 1200013E08Rik; A-Raf; Araf1; AW495444
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209341 representing NM_009703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCACCACGAGGCCCTCCTGTTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGT
  ACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCCGGGAAGGCATGAGTGTCTATGACTCTTTGGACAA
  GGCCCTGAAGGTGCGGGGTCTCAACCAGGACTGCTGTGTGGTCTACAGGCTCATCAAAGGAAGAAAGACA
  GTCACTGCCTGGGACACAGCCATTGCGCCTCTGGATGGTGAGGAGCTCATTGTGGAGGTCCTAGAAGATG
  TGCCACTGACCATGCACAATTTTGTACGGAAGACATTCTTCAGCCTGGCCTTCTGTGACTTCTGCCTTAA
  GTTTCTGTTCCATGGATTCCGCTGCCAGACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
  CCCACCGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATCTGTCTGGAG
  GCTCCAGGCAGCAGGAGGCTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGCCC
  CTTTACCCAGCAACGTGACCAGGAGCACTTCTCATTCCCTGCCCCTGCCAATCCCCCACTGCAGCGCATC
  CGCTCCACATCTACTCCTAACGTCCACATGGTCAGCACCACAGCTCCCATGGACTCCAGCCTCATGCAGT
  TTACTGCTCAGAGCTTCAGCACCGATGCTGCTGGTAGAGGTGGTGATGGAGCCCCTCGGGGTAGCCCTAG
  CCCAGCTAGTGTGTCTTCAGGGAGGAAGTCCCCACATTCCAAGTTACCTTCAGAACAGCGGGAGCGGAAG
  TCCTTGGCAGATGAAAAGAAAAAAGTGAAGAACCTGGGGTACCGGGACTCAGGCTATTACTGGGAGGTGC
  CACCCAGTGAGGTACAGCTGTTGAAGAGGATCGGGACAGGCTCTTTTGGCACTGTGTTTCGGGGGCGTTG
  GCATGGCGATGTAGCTGTGAAAGTGCTCAAGGTGGCCCAACCTACCGCTGAGCAGGCCCAGGCCTTCAAG
  AATGAGATGCAGGTGCTCAGGAAGACACGGCATGTCAACATTTTGCTGTTCATGGGTTTCATGACTCGGC
  CGGGGTTTGCCATCATCACACAGTGGTGTGAGGGTTCCAGCCTCTACCACCACCTACATGTGGCTGACAC
  GCGCTTTGACATGGTCCAGCTCATTGATGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTCCACGCC
  AAGAACATCATTCATCGAGACCTAAAGTCCAACAATATCTTCTTACATGAGGGGCTCACAGTCAAGATTG
  GTGACTTCGGCCTGGCCACAGTGAAGACACGGTGGAGTGGGGCACAGCCCTTAGAGCAGCCCTCAGGGTC
  TGTGCTATGGATGGCAGCTGAGGTGATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCGGATGTC
  TATGCCTATGGTGTTGTGCTCTATGAGCTCATGACCGGCTCACTGCCCTACAGCCACATTGGCAGCCGTG
  ACCAGATCATCTTTATGGTGGGCCGTGGCTATCTGTCTCCGGACCTCAGCAAAATCTTCAGTAATTGCCC
  CAAAGCCATGAGGCGCCTGCTGACTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTATTTCCCCAG
  ATTCTGGCCACGATCGAGCTACTGCAGCGGTCACTCCCCAAGATTGAGCGGAGTGCCTCCGAACCCTCCT
  TGCACCGCACCCAGGCTGATGAGTTGCCTGCTTGCCTTCTCAGCGCAGCCCGTCTTGTGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209341 representing NM_009703
  Red=Cloning site Green=Tags(s)
  
  MEPPRGPPVSGAEPSRAVGTVKVYLPNKQRTVVTVREGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKT
  VTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKV
  PTVCVDMSTNRRQFYHSIQDLSGGSRQQEAPSNLSVNELLTPQGPSPFTQQRDQEHFSFPAPANPPLQRI
  RSTSTPNVHMVSTTAPMDSSLMQFTAQSFSTDAAGRGGDGAPRGSPSPASVSSGRKSPHSKLPSEQRERK
  SLADEKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVAQPTAEQAQAFK
  NEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHA
  KNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDV
  YAYGVVLYELMTGSLPYSHIGSRDQIIFMVGRGYLSPDLSKIFSNCPKAMRRLLTDCLKFQREERPLFPQ
  ILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009703
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009703.1, NM_009703.2, NP_033833.1
• RefSeq Size: 2405
• RefSeq ORF: 1815
• Locus ID: 11836
• MW: 68 kDa