Klhl9 (NM_172871) Mouse Tagged ORF Clone

CAT#: MR209345

Klhl9 (Myc-DDK-tagged) - Mouse kelch-like 9 (Drosophila) (Klhl9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klhl9
• Synonyms: 8030469P05; C530050O22Rik; mKIAA1354
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209345 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGTCTCTGGGTAACGGCGACATGGGCGTCTCTGCCCACCTGCAGCCTTGCAAGTCTGGAACTA
  CGCGGTTTTTTACCAGCAACACGCACAGTTCGGTTGTATTGCAAGGCTTTGATCAGCTCAGAATAGAGGG
  CCTACTTTGTGACGTGACGCTGGTACCTGGCGATGGAGAGGAAATCTTCCCGGTTCATAGAGCCATGATG
  GCGTCTGCCAGTGATTATTTCAAGGCCATGTTCACTGGAGGAATGAAAGAGAAAGATTTGATGTGCATCA
  AGCTTCATGGGGTGAACAAAGTTGGTCTGAAGAAAATCATTGATTTTATTTATACTGCAAAACTGTCTCT
  TAATATGGACAATCTTCAGGACACTCTTGAAGCTGCCAGCTTTCTACAGATCTTGCCGGTTTTGGATTTT
  TGTAAAGTATTTCTTATATCAGGCGTCTCTTTGGATAACTGTGTGGAGGTTGGCCGGATTGCTAACACCT
  ACAATCTTATAGAAGTGGATAAATACGTAAATAATTTCATCCTGAAGAACTTTCCTGCCTTGTTGAATAC
  TGGGGAGTTTCTGAAACTCCCCTTTGAACGGCTTGCCTTTGTGCTTTCCAGCAATAGTCTCAAGCACTGT
  TCGGAACTTGAGCTCTTCAAGGCAGCCTGCCGCTGGCTGAGGCTGGAAGACCCTCGGATGGACTATGCCG
  CAAAATTAATGAAGAATATTCGCTTTCCACTGATGACACCACAGGATCTCATCAACTATGTGCAGACAGT
  AGATTTCATGAGAACAGACAATACCTGTGTGAATTTACTTTTGGAAGCAAGCAATTACCAAATGATGCCG
  TATATGCAGCCGGTGATGCAGTCGGACAGAACTGCCATTCGATCTGATTCTACTCATTTGGTTACCTTAG
  GAGGAGTTTTGAGACAACAGCTAGTTGTCAGTAAAGAGTTACGAATGTACGACGAAAGGGCGCAGGAGTG
  GAAGTCGTTAGCGCCCATGGATGCTCCTCGATACCAGCATGGCATTGCTGTTATTGGAAATTTCCTTTAT
  GTAGTTGGTGGCCAGAGTAATTATGATACAAAAGGAAAAACGGCTGTTGATACAGTTTTCAGATTTGATC
  CTCGTTATAATAAGTGGATGCAGGTGGCATCATTAAACGAAAAGCGCACATTCTTTCACCTGAGTGCCCT
  CAAAGGACATTTATATGCAGTTGGTGGGCGCAGTGCAGCTGGTGAACTGGCCACAGTAGAATGTTACAAC
  CCAAGAATGAATGAATGGAGCTACGTTGCAAAGATGAGTGAACCCCACTATGGCCATGCTGGAACAGTAT
  ATGGGGGTTTAATGTATATTTCCGGAGGAATCACTCATGACACTTTCCAAAATGAGCTCATGTGTTTTGA
  CCCAGATACAGACAAATGGACACAGAAGGCTCCTATGACTACAGTCCGAGGTCTGCATTGCATGTGTACA
  GTTGGAGACAAGCTGTATGTCATCGGTGGCAACCACTTCAGAGGAACAAGTGATTATGATGATGTCCTAA
  GCTGCGAATACTATTCGCCAACTCTTGACCAGTGGACACCAATTGCTGCTATGCTGAGAGGTCAGAGTGA
  TGTTGGAGTTGCAGTCTTTGAAAACAAAATCTATGTTGTTGGTGGATATTCTTGGAATAACCGGTGCATG
  GTAGAAATTGTCCAGAAATATGACCCAGAAAAAGACGAGTGGCATAAAGTTTTTGATCTTCCTGAGTCGC
  TGGGTGGCATTCGGGCTTGCACTCTCACAGTTTTTCCACCTGAAGAAAACCCTGGGTCACCTTCTAGAGA
  ATCACCTCTTTCAGCACCTTCAGATCATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209345 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVSLGNGDMGVSAHLQPCKSGTTRFFTSNTHSSVVLQGFDQLRIEGLLCDVTLVPGDGEEIFPVHRAMM
  ASASDYFKAMFTGGMKEKDLMCIKLHGVNKVGLKKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDF
  CKVFLISGVSLDNCVEVGRIANTYNLIEVDKYVNNFILKNFPALLNTGEFLKLPFERLAFVLSSNSLKHC
  SELELFKAACRWLRLEDPRMDYAAKLMKNIRFPLMTPQDLINYVQTVDFMRTDNTCVNLLLEASNYQMMP
  YMQPVMQSDRTAIRSDSTHLVTLGGVLRQQLVVSKELRMYDERAQEWKSLAPMDAPRYQHGIAVIGNFLY
  VVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRSAAGELATVECYN
  PRMNEWSYVAKMSEPHYGHAGTVYGGLMYISGGITHDTFQNELMCFDPDTDKWTQKAPMTTVRGLHCMCT
  VGDKLYVIGGNHFRGTSDYDDVLSCEYYSPTLDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCM
  VEIVQKYDPEKDEWHKVFDLPESLGGIRACTLTVFPPEENPGSPSRESPLSAPSDHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172871
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172871.2, NP_766459.2
• RefSeq Size: 4217 bp
• RefSeq ORF: 1854 bp
• Locus ID: 242521
• MW: 69.4 kDa