Aste1 (NM_001164828) Mouse Tagged ORF Clone

CAT#: MR209395

Aste1 (Myc-DDK-tagged) - Mouse asteroid homolog 1 (Drosophila) (Aste1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aste1
• Synonyms: 1100001A21Rik; AV006403
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209395 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTATTCTCTCTTCCACCGACTTTGCTTCAATTCTGACTTGGAGCTCAGATGGAGGATGTGACATTT
  CAGATAAAAAGCTGGCAACTTTGAAGGATCGAGCAAAAGAGAAGATCCAAGCAGCCCGTTCCCTTTCTCT
  TGGTGGGGGTGGGAATGTGTGCCCCTTACTCATCCGAGAAGTGTTCATACAGGTTTTGATCAGGCTTGAG
  GTATGTTTCGTACAGAGCTTTTCAGAAGCAGATCGGGACATTATGACACTTGCCAATCACTGGAATTGTC
  CTGTGCTATCATCGGATAGTGACTTTTGCATTTTTGACTTGAGAAGTGGGTTTTGCTCACTGAATAGCTA
  CCAGTGGAGAAATCTGAACACTATTAAAGATACACAAGATTACTACATTCCCGCCAGAAGCTTTTCCCTC
  AATGCATTCTGCCATTACTTCAACAACATGAACAAAGCTCTACTACCTCTCTTTGCGGTGCTGTGTGGAA
  ATGACCATGTTAACCTGCCCATCATGGACACATTCATAAGTAAAGTACGTCTACCCCTTAGTTCTAAGGG
  GAGGAGATATCACCGAGTCCTGGGACTTCTGAACTGGTTGTCTCATTTCGATGACCCCACTGAAGCACTA
  GATAACGTTCTGAAGTCTCTCCCCAAGAAGAGTCGAGAAAATGTTAAGGAACTTCTCTGCTGCTCCATGG
  AGGAGTACCAGCAGTCCCCGGTGAAGCTGCAGGACTTCTTCCAGTATGGTAGTTATGTCTGTACGGACGC
  TTCGGATCTGGGTCTACCAGAGTGGGTGCTAGGAGCTCTGGCCAAAGGCCAGCTACCACCTTTCATCAGT
  GATGCTTTGGTGCTCCGAAGGACCTTTCTTCACACACAGGTAGAAAACATGCAGCGGCCAAATGCTCACA
  GAATATCTCAGCCCATCCGCCAAATCATCTATGGACTCCTTTTGAATGGCCCATCACACGCAGAAGATAT
  AGCCCAGAATACATTGCCTTCCCAGCTTCTGGCTTTCAATGAAGTGGAAAGAATTGATACAAATATCAAA
  ACATCAACTGTTTATGCAAAGCAACTGCTCAAGGATCAATGTGACTTGAGCAAATTGGCTGAGCTCCCCT
  TGGCTAGGCGGCAGATGCTTCTCTTAGAAGCCCTGAAGGTCAAACAGGTTGTCCTGGAGTCCATCCCTAC
  TTTTCTGAAGTTGCCCATTGCTGTGACTTGTTACTGGTTGCAGTCCACAGAAGCCAAGGCAAAGCTCCAT
  CATCTGCAGGCCCTACTGCTGGGGATGCTGAGGGAACCCTTGCATGCCATCGTCAACAGCCCTGGCACTG
  AAGATCCACAGAGAGGTGGTGCCAAGATGTTGTATGAAGAATTATGCCAGGTGAAGGCACCCATGCGGCC
  GGGTCCAAGAGTGGATTTAGACACAGCGCACGTCTTCTGTCAGTGGCAGTCCTGTCTCCAGATGGGGTTA
  TATCTCAACCAGCTGCTGTCCACTCCTCTGCCAGAGCCAAACCTAACTTGGTTGTACAACGGAAGCCTGG
  TCCACAGACTCTGCCAGCAACTGCCAGCATCTAGTTCTGTAGAAAGCCTCCTGAGTCTATGTCCTGAGGC
  AAAGCAACTTTATGGACATCTGTTCAATGCCACAAAATCATATGCCCCAGCTGAATTGTTCTTACCAAAA
  ACTAAATCAAAATCCAAAAAAAAGAGGCAGAAGAAAAAGGTTGCCAGCCTGGGAACCACTGCAGATGCCA
  AACACTGGTATGACAGAAGCAACCGGTTTGGACCGTTAATGCCTGAAAGCTTGGAGGAGCATGTAGAGAA
  CTCAGAGCTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209395 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAILSSTDFASILTWSSDGGCDISDKKLATLKDRAKEKIQAARSLSLGGGGNVCPLLIREVFIQVLIRLE
  VCFVQSFSEADRDIMTLANHWNCPVLSSDSDFCIFDLRSGFCSLNSYQWRNLNTIKDTQDYYIPARSFSL
  NAFCHYFNNMNKALLPLFAVLCGNDHVNLPIMDTFISKVRLPLSSKGRRYHRVLGLLNWLSHFDDPTEAL
  DNVLKSLPKKSRENVKELLCCSMEEYQQSPVKLQDFFQYGSYVCTDASDLGLPEWVLGALAKGQLPPFIS
  DALVLRRTFLHTQVENMQRPNAHRISQPIRQIIYGLLLNGPSHAEDIAQNTLPSQLLAFNEVERIDTNIK
  TSTVYAKQLLKDQCDLSKLAELPLARRQMLLLEALKVKQVVLESIPTFLKLPIAVTCYWLQSTEAKAKLH
  HLQALLLGMLREPLHAIVNSPGTEDPQRGGAKMLYEELCQVKAPMRPGPRVDLDTAHVFCQWQSCLQMGL
  YLNQLLSTPLPEPNLTWLYNGSLVHRLCQQLPASSSVESLLSLCPEAKQLYGHLFNATKSYAPAELFLPK
  TKSKSKKKRQKKKVASLGTTADAKHWYDRSNRFGPLMPESLEEHVENSELE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164828
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1238 bp
• RefSeq ORF: 480 bp
• Locus ID: 66595
• MW: 68.9 kDa