Fmr1 (NM_008031) Mouse Tagged ORF Clone

CAT#: MR209428

Fmr1 (Myc-DDK-tagged) - Mouse fragile X mental retardation syndrome 1 homolog (Fmr1)

Product Images

Coomassie blue staining of purified Fmr1 protein (Cat# TP509428). The protein was produced from HEK293T cells transfected with Fmr1 cDNA clone (Cat# MR209428) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fmr1
• Synonyms: Fmr-1; FMRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209428 representing NM_008031
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGCTGGTGGTGGAAGTGCGGGGCTCCAATGGCGCTTTCTACAAGGCATTTGTAAAAGATGTCC
  ATGAAGATTCTATAACAGTTGCTTTTGAAAACAACTGGCAACCAGAGAGACAGATTCCATTCCATGATGT
  GAGATTCCCACCACCTGTAGGTTATAATAAAGATATAAACGAAAGTGATGAAGTTGAGGTTTATTCCAGA
  GCAAATGAAAAAGAGCCTTGCTGTTGGTGGTTAGCTAAAGTGAGGATGATAAAGGGTGAGTTTTATGTGA
  TAGAATATGCAGCATGTGATGCTACGTATAATGAAATTGTCACAATTGAGCGTCTACGATCTGTTAATCC
  CAACAAACCTGCTACAAAAGATACTTTCCATAAGATCAAGCTGGAGGTGCCAGAAGATTTACGACAAATG
  TGTGCCAAAGAATCAGCACATAAGGATTTTAAAAAGGCAGTTGGTGCCTTCTCTGTAACTTATGATCCAG
  AAAATTATCAGCTGGTAATTTTGTCCATCAATGAAGTCACCTCAAAGCGAGCCCACATGTTGATTGACAT
  GCACTTTCGAAGTCTGCGCACCAAGTTGTCTCTTATACTGAGAAATGAAGAAGCCAGTAAACAACTGGAG
  AGTTCAAGGCAGCTTGCCTCAAGATTTCATGAACAGTTTATCGTACGAGAAGATCTGATGGGTTTAGCTA
  TTGGTACTCATGGTGCTAATATTCAGCAAGCTAGAAAAGTGCCTGGCGTCACTGCTATTGATTTAGATGA
  GGATACCTGCACATTTCATATTTATGGAGAGGATCAAGATGCAGTGAAAAAGGCTAGAAGCTTTCTGGAA
  TTTGCTGAAGATGTCATACAGGTTCCACGAAACTTAGTAGGCAAAGTAATAGGAAAAAATGGAAAGCTGA
  TTCAAGAGATCGTGGACAAGTCAGGAGTTGTGAGGGTGAGGATTGAGGCTGAAAATGAGAAAAGTGTCCC
  ACAAGAAGAGGAAATTATGCCACCAAGTTCCCTACCTTCCAATAATTCAAGGGTTGGACCTAACTCCTCT
  GAAGAAAAGAAACATTTAGATACAAAGGAAAACACCCATTTTTCTCAACCTAACAGTACAAAAGTCCAGA
  GGGTGTTAGTGGTTTCATCAATTGTAGCAGGGGGACCCCAGAAACCTGAACCCAAGGCTTGGCAGGGTAT
  GGTACCATTTGTTTTTGTGGGAACAAAAGACAGCATCGCTAATGCCACTGTTCTTTTGGATTATCACCTG
  AACTATTTAAAGGAAGTAGACCAGTTGCGTTTGGAGAGATTACAAATTGATGAGCAGTTGCGACAAATTG
  GAGCTAGTTCTAGACCACCACCAAATCGTACAGATAAGGAAAAAGGCTATGTGACTGATGATGGTCAAGG
  AATGGGTCGAGGTAGTAGACCTTACAGAAATAGGGGGCACGGCAGACGCGGTCCTGGATATACTTCAGGA
  ACTAATTCTGAAGCATCAAATGCTTCTGAAACAGAATCTGACCACAGAGACGAACTCAGTGATTGGTCAT
  TAGCTCCAACAGAGGAAGAGAGGGAGAGCTTCCTGCGCAGAGGAGACGGACGGCGGCGTGGAGGAGGAGG
  AAGAGGACAAGGAGGAAGAGGAAGAGGAGGAGGCTTCAAAGGAAACGACGATCATTCCCGAACAGATAAT
  CGTCCACGTAATCCAAGAGAGGCTAAAGGAAGAACAGCTGATGGATCCCTGCAGAGTGCCTCCAGTGAAG
  GGAGCCGGCTGCGCACGGGTAAAGATCGTAACCAGAAGAAGGAAAAGCCAGACAGCGTAGATGGGCTGCA
  ACCGCTGGTGAATGGAGTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209428 representing NM_008031
  Red=Cloning site Green=Tags(s)
  
  MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPERQIPFHDVRFPPPVGYNKDINESDEVEVYSR
  ANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLEVPEDLRQM
  CAKESAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLILRNEEASKQLE
  SSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLE
  FAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEAENEKSVPQEEEIMPPSSLPSNNSRVGPNSS
  EEKKHLDTKENTHFSQPNSTKVQRVLVVSSIVAGGPQKPEPKAWQGMVPFVFVGTKDSIANATVLLDYHL
  NYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKGYVTDDGQGMGRGSRPYRNRGHGRRGPGYTSG
  TNSEASNASETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTDN
  RPRNPREAKGRTADGSLQSASSEGSRLRTGKDRNQKKEKPDSVDGLQPLVNGVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008031
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008031.1, NM_008031.2, NM_008031.3, NP_032057.2
• RefSeq Size: 4412
• RefSeq ORF: 1845
• Locus ID: 14265
• MW: 69.3 kDa