Dglucy (NM_145448) Mouse Tagged ORF Clone

CAT#: MR209457

9030617O03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9030617O03 gene (9030617O03Rik)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dglucy
• Synonyms: 9030617O03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209457 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATCTCATTTCTCCTGAGGTCCTGTCTTCGCTCTGCTGTAAGGAGTCTACCCAAGGCAGCACTTA
  TCAGAAACACTTCCAGCATGACGGAAGGACTCCAGCCGGCTAGTGTGGTGGTCCTGCCCAGATCCCTAGC
  ACCAGCTTTTGAAAGCTTCTGCCAGGGCAACCGGGGTCCTCTGCCCCTCCTTGGACAAAGTGAGGCGGTG
  AAGACACTCCCTCAGCTGAGCGCTGTTCCAGACATAAGGACCATCTGTCCACAGTTGCAGAAATACAAGT
  TTGGCACCTGCACAGGCATCCTGACCTCAATGGAAGAGCACTCAGAACAACTAAAAGAAATGGTGACCTT
  CATCATAGACTGCAGCTTCTTCATAGAAGAGGCCTTGGAGCAGGCAGAGATCCCCAGAAGAGACCTAACA
  GGTCCCAGCCATGCAGGAGCATACAAGACAACAGTGCCCTGTGCCACCATTGCTGGCTTCTGCTGCCCTC
  TGGTGGTCACAATGAGACCCATTCCCAAGGACAAGCTGGAAAGGCTGTTGCAGGCCACTCACGCCATAAG
  AGGACAGCAAGGACAACCCATTCACATCGGTGACCCAGGTCTTTTGGGAATTGAGGCACTTTCCAAACCT
  GACTACGGGAGTTATGTGGAGTGTCGGCCCGAGGATGTCCCTGTGTTCTGGCCATCTCCGCTGACCAGTC
  TGGAAGCAGTCATCAGCTGCAAGGCTCCATTGGCTTTCGCCAGCCCTCCAGGCTGCATGGTGATGGTCCC
  GAAGGACACAGCGTCTTCAGCCAGTTGTCTGACTCCTGAGATGGTTCCAGAAGTCCATGCCATTTCCAAA
  GACCCTTTGCATTACAGCATAGTGTCAGCCCCTGCTGCTCAAAAGGTCAGAGAGCTAGAGTCCACAATTG
  CCGTAGACCCAGGGAACCGAGGAATCGGGCACCTCCTACTTAAAGATGAGCTACTGCAAGCTGCTTTGTC
  ACTGTCTCATGCCCGCTCCGTACTCGTCACCACTGGATTCCCAACACATTTCAATCATGAGCCCCCAGAA
  GAGACAGATGGCCCACCAGGAGCCATCGCCTTAGCTGCCTTCCTACAGGCTCTGGGGAAGGAGACCGCCA
  TGGTAGTAGACCAGAGAGCCTTGAACTTGCATATGAGGATTGTTGAAGACGCCATTAGGCAAGGAGTTCT
  AAAGACACCGATTCCCATATTAACTTACCAAGGAAGATCCATGGAAGATGCTCGGGCATTTTTGTGCAAA
  GATGGGGACCCTAAGTCTCCTAGATTTGACCATCTGGTGGCCATAGAGCGTGCGGGAAGGGCTGCTGATG
  GCAATTACTACAACGCGAGGAAGATGAACATCAAACACTTAGTTGACCCCATTGATGACATTTTCCTTGC
  TGCACAAAAGATTCCTGGCATCTCATCAACTGGTGTTGGTGACGGAGGTAATGAGCTTGGAATGGGCAAA
  GTAAAGGCGGCCGTGAAGAAGCACATTAGAAATGGAGATGTCATTGCCTGTGATGTGGAGGCTGATTTTG
  CTGTCATTGCCGGTGTTTCTAACTGGGGAGGCTACGCCCTGGCCTGTGCACTGTATATTCTGAACTCATG
  TCAAGTCCATGAGCGCTACCTAAGGAGGGCAACTGGACCTTCCAGGAGAGCTGGGGAACAGAGCTGGATC
  CAGGCCCTGCCATCTGTCGCTAAGGAAGAAAAGATGTTGGGCATCCTGGTAGAGAACCAAGTCCGCAGTG
  GTGTCTCAGGCATCGTGGGCATGGAAGTGGATGGGCTGCCTTTCCATGACGTTCATGCTGAGATGATCCG
  GAAGCTGGTGGGTGCCACCACAGTGCACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209457 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTISFLLRSCLRSAVRSLPKAALIRNTSSMTEGLQPASVVVLPRSLAPAFESFCQGNRGPLPLLGQSEAV
  KTLPQLSAVPDIRTICPQLQKYKFGTCTGILTSMEEHSEQLKEMVTFIIDCSFFIEEALEQAEIPRRDLT
  GPSHAGAYKTTVPCATIAGFCCPLVVTMRPIPKDKLERLLQATHAIRGQQGQPIHIGDPGLLGIEALSKP
  DYGSYVECRPEDVPVFWPSPLTSLEAVISCKAPLAFASPPGCMVMVPKDTASSASCLTPEMVPEVHAISK
  DPLHYSIVSAPAAQKVRELESTIAVDPGNRGIGHLLLKDELLQAALSLSHARSVLVTTGFPTHFNHEPPE
  ETDGPPGAIALAAFLQALGKETAMVVDQRALNLHMRIVEDAIRQGVLKTPIPILTYQGRSMEDARAFLCK
  DGDPKSPRFDHLVAIERAGRAADGNYYNARKMNIKHLVDPIDDIFLAAQKIPGISSTGVGDGGNELGMGK
  VKAAVKKHIRNGDVIACDVEADFAVIAGVSNWGGYALACALYILNSCQVHERYLRRATGPSRRAGEQSWI
  QALPSVAKEEKMLGILVENQVRSGVSGIVGMEVDGLPFHDVHAEMIRKLVGATTVHM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145448
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145448.1, NM_145448.2, NM_145448.3, NM_145448.4, NP_663423.2
• RefSeq Size: 3229 bp
• RefSeq ORF: 1854 bp
• Locus ID: 217830
• MW: 66.5 kDa