Pias3 (NM_146135) Mouse Tagged ORF Clone

CAT#: MR209486

Pias3 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pias3
• Synonyms: Pias3l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209486 representing NM_146135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGATGAGTTTCCGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGCTGGCAGGAACAAGAGTGGGC
  GGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTCTAGCTGCGCCCCCAGCGTCCAGATGAA
  GATCAAAGAACTTTATCGCAGGCGCTTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTCCTTGCTTTCT
  TTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTGCTCCCATCCCTCCCACCCTTCTGACCC
  CTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGACATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGA
  TGTCACCATGAAGCCACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTTGCGTCC
  ACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCTCACTCCCCAGCAGCTGCAGCAGATTC
  TCACGTCCAGGGAGGTTCTGCCAGGAGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCT
  CTGTGAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAAGGTTAATGGGAAACTC
  TGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGAATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCA
  ACATCACACCCTTGGCTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATCTGAGTT
  TGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGACTGCAGGGACCCTTCTACAAAAACTC
  AGAGCCAAGGGGATCCGGAATCCAGACCATTCCCGGGCACTGATCAAGGAGAAACTGACCGCTGACCCCG
  ACAGTGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGAAGATGCGCCTGACTGT
  CCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAGAGTTTCGATGCTGCCCTTTATCTACAGATGAATGAG
  AAGAAGCCGACATGGACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTGATGGTT
  TATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGATCCAGTTCATGGAAGATGGATCCTGGTG
  TCCGATGAAACCCAAGAAGGAGGCATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAG
  TACAGCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTCATTGACTTGACCATCG
  AAAGCTCATCAGATGAGGAGGATTTGCCCCCCACCAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCC
  AGCCCTTCCTGGAAGCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGCCCTGCA
  ATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACATGAGTACCCACCTGCCTTCCCACTGG
  GGGCTGACATCCAAGGTTTAGATTTATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGT
  TATCACTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGGAACCCCTTCCCACTTC
  CTGGGCCCACTGGCCCCCACACTGGGGAGCTCTCACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTG
  TCAGCAGCATTGTGGCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGGTCCCTC
  TTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209486 representing NM_146135
  Red=Cloning site Green=Tags(s)
  
  MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLS
  LPPGTSPVGSPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLAS
  TSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKL
  CPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKL
  RAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNE
  KKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQ
  YSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPA
  MGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHF
  LGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146135
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146135.1, NM_146135.2, NP_666247.1
• RefSeq Size: 2975 bp
• RefSeq ORF: 1860 bp
• Locus ID: 229615
• MW: 67.8 kDa