Pck1 (NM_011044) Mouse Tagged Lenti ORF Clone
CAT#: MR209511L2
- LentiORF®
Lenti ORF clone of Pck1 (mGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1)
"NM_011044" in other vectors (5)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone Lentiviral Particle |
Tag | mGFP |
Symbol | Pck1 |
Synonyms | AI265463; Pck-1; PEPCK |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR209511).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_011044 |
ORF Size | 1869 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011044.2, NP_035174.1 |
RefSeq Size | 2617 bp |
RefSeq ORF | 1869 bp |
Locus ID | 18534 |
UniProt ID | Q9Z2V4 |
Cytogenetics | 2 95.79 cM |
Gene Summary | Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR209511 | Pck1 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1) |
USD 580.00 |
|
MG209511 | Pck1 (GFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1) |
USD 640.00 |
|
MR209511L1 | Lenti ORF clone of Pck1 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1) |
USD 780.00 |
|
MR209511L3 | Lenti ORF clone of Pck1 (Myc-DDK-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1) |
USD 780.00 |
|
MR209511L4 | Lenti ORF clone of Pck1 (mGFP-tagged) - Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1) |
USD 780.00 |
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