Keap1 (NM_001110307) Mouse Tagged ORF Clone
CAT#: MR209525
- TrueORF®
Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4
"NM_001110307" in other vectors (5)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Keap1 |
Synonyms | INRF2; mKIAA0132 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209525 representing NM_001110307.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGCCCGAACCCAAGCTTAGCGGGGCTCCCCGCAGCAGCCAGTTCCTGCCCCTGTGGTCAAAGTGC CCCGAGGGGGCCGGGGACGCAGTGATGTATGCCTCCACGGAGTGCAAGGCAGAGGTGACGCCCTCGCAG GACGGTAACCGAACCTTCAGCTACACACTAGAGGATCACACCAAGCAGGCTTTTGGCGTCATGAACGAG CTTCGCCTGAGCCAGCAACTCTGTGACGTGACCCTGCAGGTCAAATATGAGGACATCCCAGCTGCCCAA TTCATGGCTCACAAAGTGGTGCTGGCCTCCTCCAGCCCAGTCTTTAAAGCCATGTTCACCAACGGGCTT CGGGAGCAGGGCATGGAGGTGGTGTCCATCGAAGGCATCCACCCTAAGGTCATGGAAAGGCTTATTGAG TTCGCCTACACGGCCTCCATCTCCGTGGGCGAGAAGTGTGTCCTGCACGTGATGAACGGGGCGGTCATG TACCAGATTGACAGCGTGGTTCGAGCCTGCAGCGACTTCCTCGTGCAGCAGCTGGACCCCAGCAACGCC ATTGGCATCGCCAACTTCGCGGAGCAGATCGGCTGCACTGAACTGCACCAGCGTGCCCGGGAGTATATC TACATGCACTTCGGGGAGGTGGCCAAGCAGGAGGAGTTCTTCAACCTGTCACACTGCCAGCTGGCCACG CTCATCAGCCGGGATGATCTGAACGTACGCTGCGAGTCCGAGGTGTTCCACGCGTGCATCGACTGGGTC AAATACGACTGCCCGCAGCGGCGCTTCTACGTGCAGGCACTGCTGCGGGCCGTGCGCTGCCATGCGCTC ACGCCGCGCTTCCTGCAGACACAGCTGCAGAAGTGTGAGATCCTGCAGGCCGACGCGCGCTGCAAGGAC TACCTGGTGCAGATATTCCAGGAGCTCACGCTGCACAAGCCCACGCAGGCAGTGCCCTGCCGCGCGCCC AAAGTGGGCCGCCTCATCTACACAGCGGGCGGTTACTTCCGACAGTCGCTCAGCTACCTGGAGGCCTAC AACCCGAGCAATGGCTCCTGGCTGCGCCTGGCCGATCTACAGGTGCCGCGCAGTGGGCTGGCAGGCTGC GTGGTGGGTGGGCTGCTATACGCTGTGGGCGGCCGCAACAACTCTCCGGATGGCAACACTGACTCCAGC GCCCTGGACTGCTACAACCCCATGACCAACCAGTGGTCGCCCTGTGCCTCTATGAGCGTGCCACGCAAC CGCATCGGGGTGGGGGTCATAGATGGCCACATCTACGCAGTCGGGGGTTCCCACGGCTGCATCCACCAC AGCAGCGTGGAGAGATATGAGCCAGAGCGGGACGAGTGGCATCTAGTCGCGCCAATGTTGACACGGAGG ATTGGCGTGGGCGTGGCAGTGCTCAACCGCTTGCTGTATGCAGTGGGGGGCTTTGACGGGACTAACCGG CTTAACTCCGCAGAATGTTACTATCCAGAGAGGAATGAGTGGCGGATGATCACACCGATGAATACCATC CGGAGCGGGGCCGGGGTCTGCGTGCTGCACAACTGTATCTATGCAGCAGGGGGCTACGATGGGCAGGAC CAGTTGAACAGTGTGGAGCGCTACGACGTGGAGACAGAGACCTGGACTTTCGTAGCCCCCATGAGGCAT CACCGTAGTGCGCTGGGGATTACTGTGCACCAGGGCAAGATCTACGTCCTCGGAGGCTATGATGGCCAC ACTTTTCTGGACAGTGTGGAATGCTATGACCCGGACAGTGATACCTGGAGTGAGGTGACCCGCATGACA TCTGGCCGCAGCGGGGTGGGTGTGGCCGTCACCATGGAACCCTGTCGGAAGCAAATTGATCAACAAAAC TGTACCTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by MR209525
Blue=ORF Red=Cloning site Green=Tag(s) MQPEPKLSGAPRSSQFLPLWSKCPEGAGDAVMYASTECKAEVTPSQDGNRTFSYTLEDHTKQAFGVMNE LRLSQQLCDVTLQVKYEDIPAAQFMAHKVVLASSSPVFKAMFTNGLREQGMEVVSIEGIHPKVMERLIE FAYTASISVGEKCVLHVMNGAVMYQIDSVVRACSDFLVQQLDPSNAIGIANFAEQIGCTELHQRAREYI YMHFGEVAKQEEFFNLSHCQLATLISRDDLNVRCESEVFHACIDWVKYDCPQRRFYVQALLRAVRCHAL TPRFLQTQLQKCEILQADARCKDYLVQIFQELTLHKPTQAVPCRAPKVGRLIYTAGGYFRQSLSYLEAY NPSNGSWLRLADLQVPRSGLAGCVVGGLLYAVGGRNNSPDGNTDSSALDCYNPMTNQWSPCASMSVPRN RIGVGVIDGHIYAVGGSHGCIHHSSVERYEPERDEWHLVAPMLTRRIGVGVAVLNRLLYAVGGFDGTNR LNSAECYYPERNEWRMITPMNTIRSGAGVCVLHNCIYAAGGYDGQDQLNSVERYDVETETWTFVAPMRH HRSALGITVHQGKIYVLGGYDGHTFLDSVECYDPDSDTWSEVTRMTSGRSGVGVAVTMEPCRKQIDQQN CTC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using MR209525 also available, TP509525 |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001110307 |
ORF Size | 1872 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 3151 bp |
RefSeq ORF | 1875 bp |
Locus ID | 50868 |
UniProt ID | Q9Z2X8 |
Cytogenetics | 9 A3 |
MW | 69.6 kDa |
Gene Summary | Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219786 | Keap1 (untagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4, (10ug) |
USD 840.00 |
|
MR209525L1 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4 |
USD 840.00 |
|
MR209525L2 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4 |
USD 840.00 |
|
MR209525L3 | Lenti ORF clone of Keap1 (Myc-DDK-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4 |
USD 840.00 |
|
MR209525L4 | Lenti ORF clone of Keap1 (mGFP-tagged) - Mouse kelch-like ECH-associated protein 1 (Keap1), transcript variant 4 |
USD 840.00 |
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