Pmel (NM_021882) Mouse Tagged ORF Clone

CAT#: MR209549

Pmel (Myc-DDK-tagged) - Mouse premelanosome protein (Pmel)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pmel
• Synonyms: D10H12S53E; D12S53Eh; gp87; gp100; Pmel17; Si; Silv
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209549 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCCAGAGAAGGAGCTTCCTTCCCGTGCTTGTGCTGAGTGCTCTGCTGGCTGTGGGGGCCCTAG
  AAGGATCCAGGAATCAGGACTGGCTTGGTGTCCCAAGACAACTTGTAACTAAAACCTGGAACAGGCAGCT
  GTACCCCGAGTGGACAGAGGTGCAGGGGTCTAACTGCTGGAGAGGTGGCCAGGTATCTCTGAGGGTCATT
  AATGATGGGCCTACACTGGTTGGTGCAAATGCCTCCTTTTCCATTGCCCTGCACTTCCCTGGAAGTCAAA
  AGGTACTACCGGATGGTCAGGTTATCTGGGCCAACAACACCATCATCAATGGGAGCCAGGTGTGGGGAGG
  ACAGCCAGTGTATCCACAGGAGCCTGATGATGCCTGTGTCTTCCCTGACGGTGGACCCTGCCCATCTGGT
  CCTAAACCTCCGAAGAGAAGCTTTGTTTATGTTTGGAAGACCTGGGGAAAATACTGGCAAGTTCTGGGGG
  GTCCAGTGTCCAGGCTGAGCATTGCTACGGGCCACGCAAAGCTGGGCACACACACAATGGAAGTGACTGT
  CTACCACCGACGGGGTTCCCAGAGCTACGTGCCCCTTGCTCACGCCAGTTCAACCTTCACCATTACTGAC
  CAGGTACCTTTCTCCGTGAGTGTGTCCCAGCTACAGGCCTTGGACGGAGAGACCAAGCACTTCCTGAGAA
  ATCATCCTCTCATCTTTGCCCTTCAGCTCCACGACCCCAGTGGTTATTTGGCCGAGGCCGACCTCTCCTA
  CACATGGGACTTTGGAGATGGTACTGGGACCCTGATCTCTCGGGCACTTGATGTCACTCACACTTACCTG
  GAGTCGGGCTCAGTCACTGCCCAGGTGGTACTGCAGGCTGCCATTCCTCTTGTTTCCTGTGGTTCCTCCC
  CAGTCCCGGGTACCACAGATGGCTACATGCCAACTGCAGAAGCACCTGGAACCACATCTAGGCAAGGAAC
  CACTACAAAAGTTGTGGGTACTACACCTGGCCAGATGCCAACTACACAGCCCTCTGGAACCACAGTTGTA
  CAAATGCCAACCACAGAGGTCACAGCTACTACATCTGAGCAGATGCTGACCTCAGCGGTCATAGATACCA
  CACTGGCAGAGGTGTCAACTACAGAGGGTACAGGTACCACACCCACAAGGCCTTCTGGAACCACCGTTGC
  ACAAGCAACAACCACAGAGGGTCCAGATGCCAGCCCATTGCTGCCCACACAAAGTTCTACAGGGTCCATT
  AGCCCTCTACTGGATGACACCGACACCATAATGCTTGTGAAGAGACAAGTTCCCCTGGACTGTGTTCTAT
  ATCGATATGGTTCTTTCTCCCTCGCCCTGGACATTGTCCAGGGTATTGAAAGTGCTGAGATCCTGCAGGC
  TGTGCCATTCAGTGAAGGGGATGCATTTGAGCTGACTGTGTCCTGCCAAGGCGGGCTACCCAAGGAAGCC
  TGTATGGACATTTCATCACCAGGGTGCCAGCCCCCTGCCCAGAGGCTGTGCCAGTCTGTTCCACCGAGCC
  CAGACTGCCAGCTGGTTCTACACCAAGTGCTGAAAGGTGGCTCAGGGACATATTGCCTCAATGTGTCTTT
  GGCTGACGCCAACAGCCTGGCAGTGGCCAGCACCCAACTTGTTGTTCCTGGTCAAGACGGTGGCCTTGGG
  CAGGCTCCCTTGCTTGTAGGTATCTTGCTGGTGTTGGTGGCTGTGGTCCTTGCATCTCTGATACATAGGC
  ATAGACTTAAGAAGCAGGGCTCAGTTTCCCAAATGCCACATGGTAGCACTCACTGGCTGCGCCTGCCTCC
  GGTCTTCCGCGCCCGCGGCCTTGGAGAAAACAGCCCGCTCCTCAGTGGACAGCAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209549 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVI
  NDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSG
  PKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITD
  QVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYL
  ESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVV
  QMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSI
  SPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEA
  CMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLG
  QAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021882
• ORF Size: 1881 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021882.1, NM_021882.2, NM_021882.3, NM_021882.4, NP_068682.2
• RefSeq Size: 2130 bp
• RefSeq ORF: 1881 bp
• Locus ID: 20431
• MW: 66.3 kDa