Smpd1 (NM_011421) Mouse Tagged ORF Clone

CAT#: MR209568

Smpd1 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 1, acid lysosomal (Smpd1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smpd1
• Synonyms: A-SMase; ASM; aSMase; Zn-SMase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209568 representing NM_011421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACCACAGAGCATCATCCGGCCAGGACCACCTCAGAGCCGGCTGGGAGCAGAGACTGGAGAGGT
  CCTTACCGGCACCCAGAGTGGGACTCCTTTGGATGGGGCTGGGCTTGGCGCTGGTTCTGGCTCTGTTTGA
  CTCCACGGTTCTTTGGGTTCCTGCCAGAGCTTATCCTCTTCCTTCTGAAGGCCATTCTGTCAAATTCAGT
  GCCATAGCGCCGCCGCTCCAGAGTGCCTTTGGGTGGCAGAACCTCACTTGCCCCGCCTGCAAAGTCTTAT
  TCACTGCTCTCAACCATGGGCTGAAGAAGGAGCCCAATGTGGCACGGGTAGGCTCTGTGGCCATCAAGAT
  ATGCAAGATGCTGAACATAGCACCACTAGATGTGTGCCAGTCAGCCGTCCATCTCTTTGAGGACGATGTG
  GTGGAGGTGTGGACACGTTCAGTTCTGAGCCCATCAGAGGCTTGTGGCTTGCTTCTGGGCTCCTCTTGTG
  GACACTGGGACATCTTTTCGACTTGGAACATCTCTTTGCCATCAGTGCCGAAGCCACCCCCAAAGCCACC
  GAGCCCACCAGCCCCCGGTGCCCCTGTCAGCCGTGTCCTCTTCCTTACTGATCTACACTGGGACCATGAG
  TACCTGGAGGGCACAGACCCTTACTGTGCAGATCCACTTTGCTGCCGCCGGGGGTCCGGATGGCCACCCA
  ACTCCCAGAAGGGGGCTGGGTTCTGGGGCGAGTACAGCAAGTGTGACCTGCCCTTGCGAACGCTAGAAAG
  CCTGTTGAAAGGACTGGGCCCTGCCGGCCCTTTTGAAATGGTGTACTGGACGGGAGATATCCCTGCCCAT
  GATGTCTGGCAACAGTCTCGCCAAGATCAGCTGAGGGCCCTGACCACCATCACAGACCTCGTGAGGAAGT
  TCTTGGGCCCAGTGCCAGTGTACCCTGCTGTGGGCAACCATGAGAGCACTCCTGTCAATGGCTTCCCTCC
  TCCCTTCATAAAGGGAAACCAATCTTCACAATGGCTCTATGAAGCCATGGCCAAGGCATGGGAACCCTGG
  TTACCAGCTGATGCCCTTCACACCCTAAGAATTGGGGGCTTCTATGCCCTCACCCCACGCCCTGGCCTCC
  GCCTCATCTCTCTCAATATGAATTTTTGTTCCCGTGAGAACTTTTGGCTCTTGATCAACTCCACAGATCC
  TGCTGGACAACTCCAGTGGCTGGTGGAGGAGCTTCAGGCTGCTGAGAATCGAGGAGACAAAGTGCATATA
  ATTGGCCACATCCCTCCAGGACATTGTCTTAAGAGCTGGAGCTGGAATTATTACAAAATCATAGCCAGGT
  ATGAAAACACTCTGGCCGGTCAGTTCTTTGGCCACACTCACGTGGATGAGTTTGAGATCTTCTATGATGA
  GGAAACTCTGAGCCGACCACTAGCTGTAGCCTTCCTGGCGCCCAGTGCTACAACCTTTATCAACCTTAAC
  CCTGGCTACCGAGTTTACCAAATAGATGGAAACTACCCCGGAAGCTCTCATGTGGTCCTGGACCACGAGA
  CCTACATCTTGAATCTGACCCAGGCCAACGCAGCAGGAGGCACACCAAGCTGGAAGCGCCTCTACAGGGC
  TCGAGAAACCTATGGACTTCCAGATGCGATGCCTGCCTCCTGGCACAACCTGGTCTACCGCATGAGGGAT
  GATGAGCAACTCTTCCAGACCTTCTGGTTTCTCTACCATAAGGGCCACCCACCTTCAGAGCCCTGCGGCA
  CACCCTGCCGCCTGGCCACTCTGTGTGCCCAGCTCTCAGCCCGAGCAGACAGCCCTGCTTTGTGTCGCCA
  CTTGATGCCCAATGGGAGCCTCCCAGATGCTAATAGGTTGTGGTCACGCCCCCTGTTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209568 representing NM_011421
  Red=Cloning site Green=Tags(s)
  
  MPHHRASSGQDHLRAGWEQRLERSLPAPRVGLLWMGLGLALVLALFDSTVLWVPARAYPLPSEGHSVKFS
  AIAPPLQSAFGWQNLTCPACKVLFTALNHGLKKEPNVARVGSVAIKICKMLNIAPLDVCQSAVHLFEDDV
  VEVWTRSVLSPSEACGLLLGSSCGHWDIFSTWNISLPSVPKPPPKPPSPPAPGAPVSRVLFLTDLHWDHE
  YLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLPLRTLESLLKGLGPAGPFEMVYWTGDIPAH
  DVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFIKGNQSSQWLYEAMAKAWEPW
  LPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHI
  IGHIPPGHCLKSWSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDEETLSRPLAVAFLAPSATTFINLN
  PGYRVYQIDGNYPGSSHVVLDHETYILNLTQANAAGGTPSWKRLYRARETYGLPDAMPASWHNLVYRMRD
  DEQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPNGSLPDANRLWSRPLLC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011421
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011421.1, NM_011421.2, NP_035551.1
• RefSeq Size: 2430
• RefSeq ORF: 1884
• Locus ID: 20597
• MW: 70.4 kDa