Ccdc93 (NM_001025156) Mouse Tagged ORF Clone

CAT#: MR209592

Ccdc93 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 93 (Ccdc93), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc93
• Synonyms: 4633402D15Rik; 9230102M16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209592 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTACCCAAGGGGCCAGAGGGCCAGGGACTCCCGGAGGTGGAGACGAGAGAAGACGAAGAACAGA
  ATGTCAAGCTGACTGAAATCCTGGAGCTCTTGGTCGCAGCTGGCTATTTCAGAGCAAGAATTAAAGGCTT
  GTCTCCTTTCGACAAGGTAGTAGGAGGAATGACGTGGTGTATCACCACATGCAGCTTTGATGTGGACGTG
  GATTTGCTCTTTCAAGAAAACTCTACAATCGGTCAGAAAATTGCTCTATCGGAAAAGATTGTCTCCGTCC
  TCCCAAGGATGAAGTGTCCCCACCAGCTGGAGCCCCACCAGATCCAGGGCATGGATTTTATCCACATATT
  CCCTGTCGTGCAGTGGCTGGTGAAACGAGCTATCGAAACAAAGGAAGAGATGGGAGACTACATCCGCTCC
  TACTCCATCTCTCAGTTCCAGAAGACCTACAGCCTCCCGGAGGATGATGACTTCATAAAGAGAAAAGACA
  AGGCCGTCAAGACGGTCGTTGGCCTCTCGGATGCTTACAAGCCCCGGCGAAAGTACCGACGGCAGCGGGG
  AGCAGAGGAGCTGCCAGACGAAGAGTCCCGAGTCCACTCCACACTTCTGGAATATGGCAGGAGATACGGA
  TTTAGTCGCCAGAGCAAAACAGAGAAGGCTGAGGACAAGAAAACAGCACTTGCTGCAGGGCTGTCAGCTG
  CAGAGAAAGTGGATGCTCACGAGGAAGATGAGCTTCAGGCAGCCGAAGAGCAGCGTATTCAGTCACTGAT
  GACCAAGATGACCGCCATGGCAAACGAGGAGAGCCGTCTCACCGCAAGCTCCGTGGGCCAGATCGTGGGT
  CTCTGCTCTGAGGAGATCAAGCAGATTGTGTCTGAGTACGCAGGGAAGCAGTCTGAGCTGTCCGCTGAAG
  AAAGTCCAGAGAAGTTAGGAACCTCCCAGCTGCATCAGCGGAAAGTCATTTCCTTGAATAAGCAGATTCT
  GCAGAAGAGCAAACATCTGGAAGAGCTGCAAGCGAATCATACCAGCCTCAAAGCCAAGTACAGCGACAGG
  AAGAAGACACTGACAGAGTTGAAGGATCATGGTGAGAAACTAGACAAAGAGCAGGCAGCCCTGGAGAAAT
  TAGAAGCCAAAGCCGACCCAAGCATGCTGCAGAACCTGAGAGCACTTGTAGCCATGAATGAAAGTCTGAA
  AAGCCAGGAGCAGGAGTTTAAGGCACACTGCCGGGAGGAGATGGCTCGGCTACAACAGGAAATTGAGACC
  CTGAAAGCTGAGAGAGCACCAGGTGAGAAGATCATCTCTGGTGGAGAGCCGCAGGGTGCCCTGACCTCCA
  CCATGACTCACAATGAGGACCTGGACAGACGGTACAACATGGAAAAAGAGAAGCTGTACAAGATCCGGTT
  ACTACAGGCACGAAGAAATCGAGAAATAGCAATTTTGCATCGCAAGATTGACGAAGTGCCCAGTCGAGCA
  GAGCTGATACAGTATCAGAAGCGATTTATTGAACTCTACCGTCAGATTTCCGCAGTGCACAAGGAAACCA
  AGCAGTTCTTCACTTTGTACAATACCCTGGATGACAAAAAGGTTTACCTGGAGAAGGAGATTAGCCTGCT
  GAACTCTATTCACGAGAACTTCTCACAAGCCATGGCCTCCCCTGCTGCCCGGGACCAGTTTTTAAGGCAG
  ATGGAACAGATCGTCGAAGGCATTAAACAAAGCAGGATGAAGATGGAAAAGAAGAAGCAGGAGAACAAGA
  TGAGGAGGGACCAGTTGAACGATCAGTACTTGGAGCTCCTGGAAAAGCAGAGGCTCTACTTCAAGACTGT
  GAAAGAGTTCAAAGAGGAGGGCCGAAAGAATGAGCTATTACTATCTAAGATCAAAGCCAAGGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209592 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLPKGPEGQGLPEVETREDEEQNVKLTEILELLVAAGYFRARIKGLSPFDKVVGGMTWCITTCSFDVDV
  DLLFQENSTIGQKIALSEKIVSVLPRMKCPHQLEPHQIQGMDFIHIFPVVQWLVKRAIETKEEMGDYIRS
  YSISQFQKTYSLPEDDDFIKRKDKAVKTVVGLSDAYKPRRKYRRQRGAEELPDEESRVHSTLLEYGRRYG
  FSRQSKTEKAEDKKTALAAGLSAAEKVDAHEEDELQAAEEQRIQSLMTKMTAMANEESRLTASSVGQIVG
  LCSEEIKQIVSEYAGKQSELSAEESPEKLGTSQLHQRKVISLNKQILQKSKHLEELQANHTSLKAKYSDR
  KKTLTELKDHGEKLDKEQAALEKLEAKADPSMLQNLRALVAMNESLKSQEQEFKAHCREEMARLQQEIET
  LKAERAPGEKIISGGEPQGALTSTMTHNEDLDRRYNMEKEKLYKIRLLQARRNREIAILHRKIDEVPSRA
  ELIQYQKRFIELYRQISAVHKETKQFFTLYNTLDDKKVYLEKEISLLNSIHENFSQAMASPAARDQFLRQ
  MEQIVEGIKQSRMKMEKKKQENKMRRDQLNDQYLELLEKQRLYFKTVKEFKEEGRKNELLLSKIKAKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025156
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025156.1, NM_001025156.2, NP_001020327.1
• RefSeq Size: 7272 bp
• RefSeq ORF: 1890 bp
• Locus ID: 70829
• Cytogenetics: 1 E2.3
• MW: 72.6 kDa