Rpn2 (NM_019642) Mouse Tagged ORF Clone

CAT#: MR209608

Rpn2 (Myc-DDK-tagged) - Mouse ribophorin II (Rpn2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rpn2
• Synonyms: 1300012C06Rik; AV261018; Rpn-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209608 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCCGGGTTCAAGCGCTGTCTTCCTGTTGGCCCTGACAATCACTGCCAGCGTCCAGGCTCTGA
  CCCCCACCCACTACCTCACCAAGCAGGATGTGGAAAGGCTGAAGGCCTCACTGGACCGCCCCTTCACAGA
  TTTGGAGTCTGCCTTCTACTCCATCGTGGGACTCAGCAGCCTTGGGGTACAGGTGCCAGATGTCAAGAAA
  GCGTGTACCTTCATCAAGTCCAACCTTGATCCCAGCAACGTGGATTCTCTCTTCTATGCAGCCCAGTCCA
  GCCAGGTCCTCTCAGGCTGTGAGATATCTGTTTCAAATGAGACCAAAGAGCTCCTGCTGGCAGCAGTGAG
  TGAAGACTCCCCCATTGCCCAGATCTACCATGCAGTTGCAGCACTCAGCGGGTTTGGGCTTCCCTTGGCA
  TCCAATGAAGCCCTCGGTGCCCTTACCGCTCGCCTCGGCAAGGAAGAGACTGTGCTAGCAACGGTCCAGG
  CTCTGCAGACAGCGTCCCACCTCTCCCAGCAGGCTGACCTGAGGAACATTGTAGAGGAGATTGAGGACCT
  TGTTGCTCGGCTGGATGAACTAGGGGGTGTGTATCTGCAGTTTGAGGAAGGACTGGAGCTCACGGCGCTG
  TTTGTTGCTGCCACCTACAAGCTCATGGACCACGTGGGGACCGAACCGTCCATGAAGGAGGATCAGGTTA
  TCCAGCTCATGAACACAATCTTCAGCAAGAAGAACTTTGAGTCCCTCTCAGAAGCCTTCAGTGTGGCCTC
  TGCTGCTGCTGCATTGTCCCAGAATCGCTACCACGTACCAGTGGTGGTTGTGCCCGAGGGCTCTACTTCT
  GACACTCAAGAACAGGCTATCCTGAGGTTGCAGGTCAGCAATGTTTTGTCTCAGCCTCTGGCTCAAGCTG
  CAGTGAAGCTGGAGCATGCTAAGTCGGCGGCTACCAGGGCTACCGTCCTGCAGAAGACGCCCTTTTCGCT
  TGTGGGGAATGTTTTTGAGCTAAACTTCAAGAATGTTAAACTTTCCAGTGGCTACTATGACTTCTCTGTC
  CGAGTTGAAGGTGACAGCCGTTACATTGCAAACACTGTAGAGCTTCGAGTCAAGATCTCCACTGAAGTTG
  GCATCACAAATGTTGATCTTTCCACTGTGGATAAGGATCAGAGCATTGCACCCAAAACTACCCGGGTGAC
  CTACCCAGCCAAAGCCAAGGGTACATTCATCGCAGACAGCCATCAGAACTTTGCCCTGTTTTTCCAGCTG
  GTAGATGTGAACACCGGTGCGGAGCTCACCCCTCACCAGACGTTTGTTCGACTTCATAACCAGAAGACTG
  GCCAGGAAGTGGTGTTTGTTGCTGAGCCAGATAACAAGAATGTATATAAGTTTGAACTGGACACCTCTGA
  AAGGAAGATTGAATTTGACTCTGCCTCTGGCACCTACACACTCTACTTAATCATCGGGGATGCCACATTG
  AAGAACCCAATCCTCTGGAACGTGGCTGACGTGGTTATCAAGTTCCCTGAGGAGGAAGCCCCCTCGACTG
  TGCTGTCCCAGAGCCTGTTTACCCCAAAACAGGAAATTCAGCACCTGTTCCGAGAGCCTGAGAAGAGGCC
  CCCCACAGTGGTGTCCAATACATTCACGGCCCTCATCCTCTCGCCCTTGCTCCTGCTCTTTGCACTGTGG
  ATCCGGATTGGAGCCAATGTCTCCAACTTCACATTTGCTCCTAGCACAGTTATCTTCCACCTGGGACATG
  CTGCGATGCTGGGCCTCATGTATATCTACTGGACTCAGCTCAACATGTTCCAGACCCTCAAGTACCTGGC
  TGTCCTGGGCACTGTGACGTTTCTGGCTGGCAACCGAATGCTGGCCCAGCATGCAGTTAAGAGAACAGCA
  CAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209608 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPPGSSAVFLLALTITASVQALTPTHYLTKQDVERLKASLDRPFTDLESAFYSIVGLSSLGVQVPDVKK
  ACTFIKSNLDPSNVDSLFYAAQSSQVLSGCEISVSNETKELLLAAVSEDSPIAQIYHAVAALSGFGLPLA
  SNEALGALTARLGKEETVLATVQALQTASHLSQQADLRNIVEEIEDLVARLDELGGVYLQFEEGLELTAL
  FVAATYKLMDHVGTEPSMKEDQVIQLMNTIFSKKNFESLSEAFSVASAAAALSQNRYHVPVVVVPEGSTS
  DTQEQAILRLQVSNVLSQPLAQAAVKLEHAKSAATRATVLQKTPFSLVGNVFELNFKNVKLSSGYYDFSV
  RVEGDSRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQL
  VDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL
  KNPILWNVADVVIKFPEEEAPSTVLSQSLFTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALW
  IRIGANVSNFTFAPSTVIFHLGHAAMLGLMYIYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQHAVKRTA
  H
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019642
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019642.2, NM_019642.3, NM_019642.4, NP_062616.2
• RefSeq Size: 2716 bp
• RefSeq ORF: 1896 bp
• Locus ID: 20014
• MW: 69.1 kDa