Limk2 (NM_001034030) Mouse Tagged ORF Clone

CAT#: MR209666

Limk2 (Myc-DDK-tagged) - Mouse LIM motif-containing protein kinase 2 (Limk2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Limk2
• Synonyms: Limk-2; whe
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209666 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGGCGGGTGATGAAGCCTGGAGGTGTCGGGGCTGTGGCACCTATGTTCCCCTGAGCCAGA
  GGCTCTATAGGACTGCCAATGAAGCCTGGCATGGCTCCTGCTTCCGGTGTTCCGAATGCCAGGAATCCCT
  CACCAACTGGTACTATGAGAAGGATGGGAAGCTGTACTGCCACAAGGACTACTGGGCCAAGTTTGGAGAA
  TTCTGCCATGGGTGCTCCCTGCTGATGACGGGGCCAGCCATGGTGGCTGGGGAGTTTAAGTACCACCCAG
  AGTGCTTTGCCTGCATGAGTTGCAAGGTGATCATTGAGGATGGAGATGCATATGCCCTGGTGCAGCACGC
  TACACTCTACTGTGGGAAGTGCCACAACGAGGTGGTGCTGGCACCCATGTTTGAAAGACTGTCTACAGAG
  TCTGTCCAGGATCAGCTGCCCTACTCTGTCACGCTCATCTCCATGCCTGCTACCACCGAGTGTAGACGCG
  GCTTCTCGGTGACTGTGGAAAGTGCCTCTTCTAACTATGCCACCACCGTTCAAGTGAAAGAGGTCAACCG
  GATGCACATCAGTCCCAACAACCGAAATGCCATCCACCCTGGGGACCGCATCTTGGAGATCAATGGGACC
  CCTGTCCGTACTCTGCGAGTAGAGGAGGTGGAGGATGCAATAAAGCAGACAAGCCAGACACTTCAGCTGC
  TGATTGAACATGACCCTGTGCCCCAGCGCCTGGACCAGCTACGGCTAGATGCCCGACTTCCTCCCCACAT
  GCAGAGCACCGGACACACGCTCATGCTCAGCACCTTGGACACCAAGGAGAATCAGGAGGGGACACTGAGG
  AGACGTTCTCTGAGGCGCAGTAACAGCATCTCCAAGTCTCCGGGCCCCAGCTCCCCCAAGGAGCCCCTGC
  TCCTCAGCCGGGACATAAGCCGCTCAGAATCCCTCCGCTGCTCTAGCAGCTACTCCCAGCAGATCTTCCG
  GCCCTGCGACCTGATCCACGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCCATCAAGGTGACTCAC
  AAAGCCACAGGCAAAGTGATGGTCATGAAGGAGTTGATCCGCTGTGATGAGGAAACCCAGAAGACTTTCC
  TGACTGAGGTGAAAGTGATGCGGAGCCTGGACCACCCTAATGTGCTCAAGTTCATCGGAGTTCTGTACAA
  GGACAAGAAGCTGAATCTGCTGACCGAGTACATCGAGGGAGGCACACTCAAAGACTTCCTGCGCAGTGTG
  GACCCGTTCCCCTGGCAACAGAAGGTCAGGTTTGCCAAAGGCATCTCCTCTGGAATGGCCTATTTACACT
  CCATGTGCATCATCCACCGGGACCTGAACTCACACAACTGTCTCATCAAATTGGACAAGACAGTGGTAGT
  AGCCGACTTTGGGCTGTCACGGCTTATAGTAGAAGAGAGGAAAAGGCCTCCAGTAGAGAAGGCCACCACC
  AAGAAACGCACCTTACGCAAGAGTGACCGCAAAAAGCGCTACACTGTGGTGGGAAACCCCTACTGGATGG
  CCCCCGAGATGCTGAATGGCAAGAGCTACGATGAGACGGTGGATGTCTTCTCTTTTGGGATCGTTCTCTG
  TGAGATCATTGGGCAGGTATATGCGGATCCTGATTGCCTGCCCCGCACACTGGACTTTGGCCTCAATGTT
  AAGCTTTTCTGGGAGAAGTTTGTCCCAACAGACTGTCCCCCAGCCTTCTTCCCCCTGGCTGCTATCTGCT
  GCAAACTAGAGCCTGAAAGCAGACCGGCATTCTCAAAGCTGGAGGACTCGTTCGAGGCGCTCTCCCTGTT
  CTTGGGGGAGCTGGCCATCCCACTGCCAGCAGAGCTGGAAGACCTGGACCACACTGTGAGCATGGAGTAT
  GGCCTAACCCGGGACTCGCCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209666 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALAGDEAWRCRGCGTYVPLSQRLYRTANEAWHGSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGE
  FCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTE
  SVQDQLPYSVTLISMPATTECRRGFSVTVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGT
  PVRTLRVEEVEDAIKQTSQTLQLLIEHDPVPQRLDQLRLDARLPPHMQSTGHTLMLSTLDTKENQEGTLR
  RRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTH
  KATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSV
  DPFPWQQKVRFAKGISSGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKATT
  KKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNV
  KLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEDLDHTVSMEY
  GLTRDSPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034030
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034030.1, NM_001034030.2, NP_001029202.1
• RefSeq Size: 3898 bp
• RefSeq ORF: 1356 bp
• Locus ID: 16886
• Cytogenetics: 11 A1
• MW: 72.2 kDa