Uba2 (NM_016682) Mouse Tagged ORF Clone

CAT#: MR209672

Uba2 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 2 (Uba2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uba2
• Synonyms: AA986091; Arx; Sae2; UBA1; Ubl1a2; Uble1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209672 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGTCGCGGGGGTTGCCCCGGGAGCTGGCCGAGGCGGTGTCCGGCGGCCGGGTGCTGGTGGTTG
  GCGCGGGTGGGATCGGCTGTGAGCTGCTCAAGAACCTGGTGCTCACTGGCTTCTCCCACATCGACCTGAT
  TGATCTGGACACTATCGATGTCAGCAACCTCAACAGGCAGTTCTTGTTTCAAAAGAAACATGTGGGAAGA
  TCAAAGGCTCAGGTTGCCAAAGAAAGTGTGCTCCAGTTCCACCCCCAAGCCAACATTGAGGCTCACCATG
  ACAGCATCATGAACCCAGACTATAATGTGGAGTTTTTTCGACAGTTTATATTGGTCATGAATGCATTAGA
  TAACAGAGCTGCACGAAACCACGTGAATAGGATGTGTCTGGCCGCTGATGTGCCTCTCATTGAGAGCGGG
  ACTGCTGGGTATCTCGGACAGGTGACCACTATCAAGAAGGGTGTGACTGAGTGTTACGAATGTCACCCTA
  AGCCTACCCAGAGGACGTTCCCTGGCTGTACGATTCGGAACACACCTTCAGAACCTATCCATTGCATCGT
  ATGGGCCAAGTATTTGTTCAACCAGCTGTTTGGAGAGGAAGATGCTGATCAAGAAGTGTCTCCTGACAGA
  GCTGACCCTGAGGCTGCTTGGGAACCAACAGAGGCTGAAGCTAGAGCTCGAGCATCTAATGAAGACGGAG
  ACATTAAGCGTATTTCCACTAAGGAATGGGCTAAGTCAACTGGATATGATCCAGTTAAACTTTTTACCAA
  GCTTTTTAAAGATGATATTAGATATCTGCTGACAATGGACAAACTCTGGCGCAAGAGGAAACCTCCAGTT
  CCACTGGACTGGGCTGAAGTGCAGAGTCAAGGAGAAGCCAATGCCGATCAACAAAATGAGCCCCAGTTGG
  GTTTGAAAGACCAGCAGGTTCTGGATGTAAAGAGCTATGCGAGTCTGTTTTCAAAGAGCATCGAGACTCT
  GCGAGTCCATTTAGCGGAGAAGGGGGATGGAGCTGAGCTCATCTGGGATAAGGACGACCCACCTGCAATG
  GATTTTGTTACATCTGCTGCAAACCTCAGGATGCACATTTTCAGCATGAATATGAAGAGCAGGTTTGATA
  TAAAATCAATGGCAGGGAATATCATTCCTGCTATAGCTACTACTAATGCAGTGATCGCCGGCTTGATAGT
  GTTGGAAGGATTAAAGATTTTATCTGGAAAAATAGACCAGTGTAGAACTATTTTTTTGAATAAGCAGCCA
  AACCCAAGAAAGAAGCTCTTGGTGCCCTGTGCACTGGATCCTCCCAACACCAACTGTTACGTTTGTGCCA
  GCAAGCCAGAGGTGACTGTGCGGCTGAATGTCCACAAAGTGACTGTCCTCACCTTACAGGATAAGATAGT
  GAAAGAAAAGTTTGCTATGGTGGCACCAGATGTCCAAATTGAAGATGGGAAAGGCACAATCCTGATATCT
  TCAGAAGAGGGAGAGACAGAAGCCAATAACCCCAAGAAGTTGTCTGATTTTGGAATTAGAAACGGCAGCC
  GGCTTCAAGCAGACGACTTCCTCCAGGACTATACTTTGCTGATCAACATTCTTCATAGTGAAGATCTTGG
  AAAGGATGTTGAATTTGAAGTAGTTGGTGACAGCCCTGAAAAAGTGGGGCCAAAACAAGCTGAAGATGCT
  GCCAAAAGCATTGCCAACGGCAGCGATGACGGTGCCCAGCCTTCCACCTCCACAGCACAAGAGCAAGATG
  ATGTGCTCATAGTTGATTCAGACGAAGAAGGCCCTTCAAATAGCACCGACTGCAGCGGGGACGACAAGGC
  CCGCAAGCGGAAGCTGGAAGAGAATGAGGCTGCCAGTACCAAGAAGTGCCGCTTGGAGCAGATGGAAGAT
  CCAGATGACGTCATAGCGTTAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209672 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSRGLPRELAEAVSGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGR
  SKAQVAKESVLQFHPQANIEAHHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADVPLIESG
  TAGYLGQVTTIKKGVTECYECHPKPTQRTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDR
  ADPEAAWEPTEAEARARASNEDGDIKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPV
  PLDWAEVQSQGEANADQQNEPQLGLKDQQVLDVKSYASLFSKSIETLRVHLAEKGDGAELIWDKDDPPAM
  DFVTSAANLRMHIFSMNMKSRFDIKSMAGNIIPAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQP
  NPRKKLLVPCALDPPNTNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIEDGKGTILIS
  SEEGETEANNPKKLSDFGIRNGSRLQADDFLQDYTLLINILHSEDLGKDVEFEVVGDSPEKVGPKQAEDA
  AKSIANGSDDGAQPSTSTAQEQDDVLIVDSDEEGPSNSTDCSGDDKARKRKLEENEAASTKKCRLEQMED
  PDDVIALD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016682
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016682.1, NM_016682.2, NP_057891.1
• RefSeq Size: 2524 bp
• RefSeq ORF: 1917 bp
• Locus ID: 50995
• MW: 70.6 kDa