Alas1 (NM_020559) Mouse Tagged ORF Clone

CAT#: MR209701

Alas1 (Myc-DDK-tagged) - Mouse aminolevulinic acid synthase 1 (Alas1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alas1
• Synonyms: ALAS; Alas-1; Alas-h; ALAS-N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209701 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACTGTCGTTCGCCGATGCCCATTCTTATCCCGAGTCCCTCAGGCCTTTCTGCAGAAGGCAGGAA
  AGTCTCTGTTGTTCTATGCTCAAAACTGCCCCAAGATGATGGAAGTTGGGGCCAAGCCAGCTCCTCGAAC
  CCTGTCCACATCAGCTGTCCACTGCCAGCAGGTCAAAGAAACCCCTCCAGCCAATGAGAAAGAGAAAACT
  GCCAAGGCCGCAGTCCAGCAGGCTCCTGACGAGTCCCAGATGGCACAGACTCCAGACGGCACACAGCTCC
  CGTCTGGACACCCGTCCCCTGCTACAAGCCAGGGCTCTGGGAGCAAGTGCCCTTTCCTGGCAGCACAGCT
  GAGCCAGACAGGCAGCAGCGTCTTCCGCAAGGCCAGTCTGGAGCTCCAGGAGGACGTGCAGGAAATGCAT
  GCTGTGAGGAAAGAGGCTGCTCAAAGCCCAGTGCCTCCCAGCCTGGTTAATGTGAAAACCGATGGAGAGG
  ATCCAAGCCGACTGCTGAAGAACTTCCAGGACATCATGCGAAAGCAGAGGCCAGAAAGAGTGTCTCATCT
  TCTTCAGGATAACTTGCCAAAGTCTGTTTCTACTTTTCAATATGATCATTTCTTTGAGAAGAAAATTGAT
  GAGAAAAAAAATGACCATACCTACCGAGTTTTTAAAACGGTGAACCGGAGAGCACAAATCTTCCCGATGG
  CGGATGACTACACGGACTCCCTCATAACCAAAAAGCAGGTGTCGGTCTGGTGCAGTAATGACTACCTAGG
  CATGAGTCGACACCCACGGGTGTGTGGGGCTGTCATGGAGACTGTGAAACAGCATGGTGCTGGAGCTGGG
  GGAACTAGGAATATTTCTGGAACGAGCAAGTTCCACGTAGAACTGGAGCAGGCACTGGCCGACCTCCACG
  GCAAAGATGCGGCGCTCTTGTTCTCTTCCTGTTTTGTGGCCAACGACTCCACCCTCTTCACCCTGGCTAA
  GATGATGCCAGGCTGTGAAATTTACTCTGATTCCGGGAACCATGCCTCCATGATCCAAGGGATTCGCAAC
  AGTCGAGTGCCAAAATATATCTTCCGCCACAATGACGTTAACCATCTCAGAGAACTGCTGCAGAGATCTG
  ACCCCTCAGTCCCCAAGATAGTAGCATTTGAAACTGTCCATTCAATGGATGCAGTGTGCCCACTGGAAGA
  GCTGTGTGACGTGGCCCATGAGTTTGGGGCAATCACGTTTGTGGATGAGGTCCATGCAGTGGGGCTCTAT
  GGAGCTCGAGGTGGAGGGATCGGTGATCGGGATGGCGTCATGCCAAAAATGGACATCATCTCTGGGACGC
  TTGGTAAAGCGTTCGGCTGTGTTGGAGGATACATTGCCAGCACGAGTTTGCTGATCGACACCGTCCGCTC
  CTACGCTGCGGGCTTCATCTTCACCACCTCCTTGCCACCAATGCTGCTGGCTGGAGCCCTGGAGTCTGTG
  CGGATCCTGAAGAGCAGCGAGGGGCGCGCCCTTCGCCGCCAGCACCAGCGAAATGTCAAGCTTCTGAGGC
  AGATGCTAATGGACGCTGGCCTCCCGGTCATCCACTGTCCGAGTCACATCATCCCTGTGCGGGTTGCCGA
  TGCTGCTAAGAACACAGAGATCTGTGATGAGTTGATGACCAGGCATAATATCTACGTCCAGGCCATCAAT
  TACCCAACAGTGCCTCGCGGGGAGGAGCTCCTGCGGATCGCCCCCACCCCGCATCACACCCCGCAGATGA
  TGAACTTCTTTGTGGAGAAGCTGCTGGTCACGTGGAAGCGAGTTGGGCTGGAACTGAAGCCACATTCGTC
  AGCTGAATGCAACTTCTGCAGGAGGCCCCTGCACTTTGAAGTGATGAGCGAAAGAGAGAAAGCCTATTTC
  TCAGGCATGAGCAAGATGGTGTCTGCCCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209701 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METVVRRCPFLSRVPQAFLQKAGKSLLFYAQNCPKMMEVGAKPAPRTLSTSAVHCQQVKETPPANEKEKT
  AKAAVQQAPDESQMAQTPDGTQLPSGHPSPATSQGSGSKCPFLAAQLSQTGSSVFRKASLELQEDVQEMH
  AVRKEAAQSPVPPSLVNVKTDGEDPSRLLKNFQDIMRKQRPERVSHLLQDNLPKSVSTFQYDHFFEKKID
  EKKNDHTYRVFKTVNRRAQIFPMADDYTDSLITKKQVSVWCSNDYLGMSRHPRVCGAVMETVKQHGAGAG
  GTRNISGTSKFHVELEQALADLHGKDAALLFSSCFVANDSTLFTLAKMMPGCEIYSDSGNHASMIQGIRN
  SRVPKYIFRHNDVNHLRELLQRSDPSVPKIVAFETVHSMDAVCPLEELCDVAHEFGAITFVDEVHAVGLY
  GARGGGIGDRDGVMPKMDIISGTLGKAFGCVGGYIASTSLLIDTVRSYAAGFIFTTSLPPMLLAGALESV
  RILKSSEGRALRRQHQRNVKLLRQMLMDAGLPVIHCPSHIIPVRVADAAKNTEICDELMTRHNIYVQAIN
  YPTVPRGEELLRIAPTPHHTPQMMNFFVEKLLVTWKRVGLELKPHSSAECNFCRRPLHFEVMSEREKAYF
  SGMSKMVSAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020559
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020559.1, NM_020559.2, NP_065584.2
• RefSeq Size: 2702
• RefSeq ORF: 1929
• Locus ID: 11655
• MW: 71 kDa