Wee1 (NM_009516) Mouse Tagged ORF Clone

CAT#: MR209724

Wee1 (Myc-DDK-tagged) - Mouse WEE 1 homolog 1 (S. pombe) (Wee1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wee1
• Synonyms: Wee1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209724 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCCGCGTAGGAGCCGCTTACAGTCTGCGGCAGA
  AGCTCATCTTCTCGCCGGGCAGCGACTGTGAGGAAGAGGAGGAGGAGGAGGAGGAAGGCAGCGGCCACAG
  TACGGGGGAGGACTCGGCCTTCCAGGAACCGGATTCGCCCCTGCCTTCTGCACGCAGCCCTGCCGAGGCC
  GAGGCCGAGCGCCGCCGTCGCTCGCCCGGCGCGGAGCCCAGCAGCCCGGGAGAGCTGGAGGACGACTTGC
  TGCTGCAGGGCGGCGGCGGAGGCGCGCAAGCGGCGGGTGGAGGCGCCGAGGGCGACTCTTGGGAGGAGGA
  GGGTTTCGGCTCCTCGTCGCCCGTCAAATCACCTTCGACCGCCTACTTTCTGAGCAGCCCGTTCTCGCCA
  GTGCGCTGCGGCGGCCCAGGGGATGCCTCCCCGCAGGGTTGCGGGGCTCCGCGAGCCATGGACGACCCCT
  GCTCGCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAAGACCTTCCGCAAGCTGCGCCTGTTCGACAC
  GCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCGCGAGTTATTGATTCCGGCTCTGTTAAACTCCGGGGT
  AGTTCTCTATTCATGGACACAGAAAAGTCAGGAAAAAGAGAATTCGATACACGGCAAACTCCCCAAGTGA
  ATATTAACCCCTTTACTCCGGATCCTGTACTGCTCCATTCCTCAGGACGGTGTCGTGGGAGAAAGAGAGC
  GTATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGATTATGAGTTTGAAGATGAAACAAGACCTGCC
  AAAAGAATTACAATTACTGAAAGCAACATGAAGTCACGGTATACAACTGAATTTCATGAGCTGGAGAAAA
  TTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAAGAGGTTAGATGGATGCATTTATGCCATTAA
  ACGATCAAAAAAACCATTGGCTGGCTCTGTTGATGAGCAGAATGCTTTGAGAGAAGTGTATGCTCACGCT
  GTGCTTGGACAGCACCCCCACGTCGTTCGCTATTTCTCTGCCTGGGCAGAGGATGACCACATGCTTATAC
  AGAACGAATACTGTAATGGTGGGAGTTTAGCTGATGCTATAAGTGAGAACTACAGAGTCATGAGCTACTT
  GACTGAAGTAGAGCTGAAGGATCTCCTTTTGCAAGTTGGCCGGGGCTTGAGATACATACATTCAATGTCT
  TTGGTTCACATGGATATAAAACCTAGTAATATTTTTATATCTCGAACCTCAATCCCAAATGCTGTCTCTG
  AGGAAGGAGATGAAGATGACTGGATATCCAATAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGTAAC
  AAGAATCTCTAGTCCTCAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGTTTTACAAGAGAAC
  TATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCTGCTGGTGCTGAAC
  CCCTTCCCAGAAATGGAGAGCAGTGGCACGAGATCCGGCAGGGTCGGTTACCTCGGATCCCACAAGTGCT
  TTCCCAGGAGGTGACAGAGTTGCTGAGAGTCATGATTCATCCTGACCCAGAGAGAAGGCCTTCAGCAATG
  GAGCTGGTGAAGCATTCAGTGTTGTTGTCTGCATCTAGAAAGAGCGCAGAGCAGTTACGAATAGAATTGA
  ATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGGCAGCCAAAGTTGC
  AGCTGAGGAACGGGCACTCTTCACAGATCGGATGGCCACTAGATCCACCACCCAGAGTAATAGAACTTCT
  CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209724 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEA
  EAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSP
  VRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRG
  SSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPA
  KRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHA
  VLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRVMSYLTEVELKDLLLQVGRGLRYIHSMS
  LVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQEN
  YSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAM
  ELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTS
  RLIGKKMNRSVSLTIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009516
• ORF Size: 1941 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009516.1, NM_009516.2, NM_009516.3, NP_033542.2
• RefSeq Size: 3419
• RefSeq ORF: 1941
• Locus ID: 22390
• MW: 71.6 kDa