Hspa8 (BC085486) Mouse Tagged ORF Clone

CAT#: MR209728

Hspa8 (Myc-DDK-tagged) - Mouse heat shock protein 8 (cDNA clone MGC:102008 IMAGE:30540914)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa8
• Synonyms: Hsc73, Hsc71, Hsp73, Hspa10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209728 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAAGGGACCTGCAGTTGGCATTGATCTCGGCACCACCTACTCCTGTGTGGGTGTCTTCCAGCATG
  GAAAGGTGGAAATTATTGCCAATGACCAGGGTAACCGCACCACGCCAAGCTATGTTGCTTTCACGGACAC
  AGAGAGATTAATTGGGGATGCGGCCAAGAATCAGGTTGCAATGAACCCCACCAACACAGTTTTTGATGCC
  AAACGTCTGATCGGGCGTAGGTTTGATGATGCTGTTGTTCAGTCTGATATGAAGCACTGGCCCTTCATGG
  TGGTGAATGATGCAGGCAGGCCCAAGGTCCAAGTGGAATACAAAGGGGAGACAAAAAGTTTCTACCCAGA
  GGAAGTGTCCTCCATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCGTACCTCGGAAAGACCGTTACC
  AACGCTGTGGTCACAGTGCCCGCTTACTTCAATGACTCTCAGCGACAGGCAACAAAAGATGCTGGAACTA
  TTGCTGGCCTCAATGTACTTCGAATCATCAATGAACCAACTGCTGCTGCTATTGCTTACGGCTTAGATAA
  GAAGGTCGGAGCTGAAAGGAATGTGCTCATTTTTGACTTGGGAGGTGGCACTTTTGATGTGTCAATCCTC
  ACTATTGAGGATGGAATTTTTGAGGTCAAATCAACAGCTGGAGACACCCACTTAGGTGGAGAAGATTTTG
  ACAACCGAATGGTCAATCATTTCATTGCTGAGTTCAAGCGAAAGCACAAGAAAGACATCAGTGAGAACAA
  GAGAGCTGTCCGCCGTCTCCGCACGGCCTGCGAGCGGGCCAAGCGCACCCTCTCCTCCAGCACCCAGGCC
  AGTATTGAGATTGATTCTCTCTATGAGGGAATTGACTTCTATACCTCCATTACCCGGGCTCGATTTGAGG
  AGTTGAATGCTGACCTGTTCCGTGGCACACTGGACCCTGTAGAGAAGGCCCTTCGAGATGCCAAGCTGGA
  CAAGTCACAGATCCATGATATTGTCTTGGTGGGTGGTTCTACCAGAATCCCCAAGATTCAGAAACTTCTG
  CAAGACTTCTTCAATGGAAAAGAGCTGAACAAGAGCATTAACCCCGATGAAGCTGTTGCCTATGGTGCAG
  CTGTCCAGGCAGCCATTCTATCTGGAGACAAGTCTGAGAACGTTCAGGATTTGCTGCTCTTGGATGTCAC
  TCCTCTTTCCCTTGGTATTGAAACTGCTGGCGGAGTCATGACTGTCCTCATCAAGCGCAATACCACCATC
  CCCACCAAGCAGACACAGACTTTCACCACCTACTCTGACAACCAGCCTGGTGTACTCATTCAGGTGTATG
  AAGGTGAAAGGGCCATGACCAAGGACAACAACCTGCTTGGAAAGTTCGAGCTCACAGGCATCCCTCCAGC
  ACCCCGTGGGGTCCCTCAGATTGAGGTTACTTTTGACATCGATGCCAATGGCATCCTCAATGTTTCTGCT
  GTAGATAAGAGCACAGGAAAGGAGAACAAGATCACCATCACCAATGACAAGGGCCGCTTGAGTAAGGAAG
  ATATTGAGCGCATGGTCCAAGAAGCTGAGAAGTACAAGGCTGAGGATGAGAAGCAGAGAGATAAGGTTTC
  CTCCAAGAACTCACTGGAGTCCTATGCCTTCAACATGAAAGCAACTGTGGAAGATGAGAAACTTCAAGGC
  AAGATCAATGATGAGGACAAACAGAAGATTCTTGACAAGTGCAATGAAATCATCAGCTGGCTGGATAAGA
  ACCAGACTGCAGAGAAGGAAGAATTTGAGCATCAGCAGAAAGAACTGGAGAAAGTCTGCAACCCTATCAT
  TACCAAGCTGTACCAGAGTGCAGGTGGCATGCCTGGGGGAATGCCTGGTGGCTTCCCAGGTGGAGGAGCT
  CCCCCATCTGGTGGTGCTTCTTCAGGCCCCACCATTGAAGAGGTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209728 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDA
  KRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVT
  NAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSIL
  TIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQA
  SIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLL
  QDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTI
  PTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSA
  VDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQG
  KINDEDKQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGA
  PPSGGASSGPTIEEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC085486
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC085486, AAH85486
• RefSeq Size: 2129
• RefSeq ORF: 1940
• Locus ID: 15481
• MW: 70.9 kDa