Grk2 (NM_130863) Mouse Tagged ORF Clone

CAT#: MR209736

Adrbk1 (Myc-DDK-tagged) - Mouse adrenergic receptor kinase, beta 1 (Adrbk1)

Product Images

Coomassie blue staining of purified Grk2 protein (Cat# TP509736). The protein was produced from HEK293T cells transfected with Grk2 cDNA clone (Cat# MR209736) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grk2
• Synonyms: Adrbk-1; Adrbk1; Bark-1; beta ARK; betaARK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209736 representing NM_130863
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGTATCTGGAGGACCGAGGAGAAGTGACCTTTGAAAAGATCTTTTCGCAGAAGTTAGGGTACC
  TGCTTTTCCGGGACTTCTGCCTGAACCATCTGGAAGAGGCCAAGCCCTTGGTGGAGTTCTACGAAGAGAT
  CAAGAAATATGAGAAGCTGGAGACAGAGGAGGAACGTGTGGTCCGCAGCCGAGAGATCTTTGACTCCTAT
  ATTATGAAAGAACTGCTGGCCTGCTCACATCCCTTTTCAAAGAATGCTACGGAGCATGTCCAGGGCCACC
  TGGTGAAGAAGCAGGTGCCTCCAGATCTCTTCCAGCCATACATTGAGGAGATTTGTCAGAACCTCCGAGG
  GGACGTGTTCCAGAAGTTCATAGAGAGTGACAAGTTCACACGGTTCTGCCAGTGGAAGAATGTTGAGCTC
  AACATCCACCTGACCATGAATGACTTCAGTGTGCATCGAATCATCGGGCGTGGGGGCTTCGGTGAGGTCT
  ATGGGTGCCGGAAAGCAGACACAGGCAAGATGTACGCCATGAAGTGTCTGGACAAGAAACGCATCAAGAT
  GAAGCAGGGAGAGACCCTGGCTCTGAACGAGCGGATCATGCTTTCCCTCGTCAGCACCGGGGACTGCCCC
  TTCATCGTGTGCATGTCATATGCATTCCACACACCAGACAAGCTCAGCTTCATCCTGGACCTCATGAACG
  GTGGGGACCTGCACTACCACCTGTCTCAGCATGGAGTCTTCTCTGAAGCCGACATGCGCTTCTACGCCGC
  TGAGATCATCCTGGGCCTTGAACACATGCACAATCGCTTTGTAGTCTACAGGGACCTGAAGCCAGCCAAC
  ATTCTCCTGGATGAACATGGCCATGTGAGAATCTCGGACCTGGGCCTAGCCTGTGACTTCTCCAAGAAGA
  GGCCTCATGCCAGTGTGGGCACACACGGGTACATGGCCCCCGAAGTCCTACAGAAGGGCGTGGCCTATGA
  CAGCAGTGCAGACTGGTTCTCCCTGGGCTGCATGCTCTTCAAGTTGTTGCGGGGACACAGCCCCTTCCGG
  CAGCACAAGACCAAAGACAAGCATGAGATTGACCGCATGACGTTGACGATGGCTGTAGAGCTGCCTGACT
  CCTTCTCCCCTGAACTCCGATCCCTGCTGGAAGGTTTGTTGCAGAGGGACGTCAATCGGAGACTAGGCTG
  TCTGGGCCGTGGGGCTCAGGAGGTAAAAGAAAGTCCTTTCTTTCGTTCCCTGGACTGGCAGATGGTCTTC
  TTACAGAAGTACCCTCCTCCACTGATCCCCCCACGTGGGGAGGTGAATGCAGCTGACGCTTTCGACATTG
  GCTCCTTTGATGAGGAGGACACAAAAGGAATCAAGTTACTGGACAGTGACCAGGAACTGTACCGCAACTT
  CCCTCTCACCATCTCTGAGCGGTGGCAACAGGAGGTGGCAGAGACTGTCTTTGATACCATCAATGCTGAA
  ACAGACCGGCTGGAGGCACGAAAGAAGGCCAAAAACAAACAGCTGGGCCACGAGGAGGACTATGCCCTGG
  GTAAGGACTGCATCGTGCATGGCTACATGTCCAAGATGGGCAACCCCTTCCTGACCCAGTGGCAGCGGCG
  ATACTTCTACTTGTTCCCCAACCGGCTCGAGTGGAGGGGTGAAGGCGAGGCTCCGCAGAGCCTCCTGACC
  ATGGAGGAGATCCAGTCAGTGGAAGAGACACAGATCAAGGAACGCAAGTGTCTCCTGCTTAAGATCCGAG
  GTGGCAAGCAGTTTGTCCTGCAGTGTGATAGTGATCCAGAGCTGGTGCAATGGAAAAAGGAGCTGCGTGA
  TGCCTACCGCGAGGCCCAGCAGCTGGTACAGCGAGTACCCAAGATGAAGAACAAGCCACGCTCGCCTGTG
  GTGGAGCTGAGCAAGGTGCCGCTGATCCAGCGTGGCAGTGCCAACGGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209736 representing NM_130863
  Red=Cloning site Green=Tags(s)
  
  MQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSY
  IMKELLACSHPFSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVEL
  NIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCP
  FIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPAN
  ILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFR
  QHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVF
  LQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAE
  TDRLEARKKAKNKQLGHEEDYALGKDCIVHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLT
  MEEIQSVEETQIKERKCLLLKIRGGKQFVLQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPV
  VELSKVPLIQRGSANGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130863
• ORF Size: 1941 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130863.1, NM_130863.2, NP_570933.1
• RefSeq Size: 3332
• RefSeq ORF: 1944
• Locus ID: 110355
• MW: 75.6 kDa
• Gene Summary: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. [UniProtKB/Swiss-Prot Function]