Mthfr (NM_010840) Mouse Tagged ORF Clone

CAT#: MR209788

Mthfr (Myc-DDK-tagged) - Mouse 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mthfr
• Synonyms: AI323986
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209788 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACGAGGCCAGAGGAAGTGGCAGTCCCAACCCGCGATCTGAGGGCAGCAGCAGTGGCAGCGAGA
  GTTCCAAGGACAGTTCAAGATGTTCCACCCCCAGCCTGGACCCAGAGCGGCACGAGAGACTCCGGGAGAA
  GATGAGGCGCAGAATGGACTCTGGTGACAAGTGGTTCTCCCTGGAGTTCTTCCCCCCTCGAACTGCTGAG
  GGAGCTGTTAACCTCATCTCCAGGTTTGACCGGATGGCAGCAGGGGGCCCCCTCTTCGTAGATGTTACCT
  GGCACCCAGCTGGAGACCCTGGCTCAGACAAGGAGACCTCCTCCATGATGATCGCCAGCACAGCAGTAAA
  CTACTGCGGCTTGGAAACCATCCTGCATATGACCTGCTGCCAGCAGCGCCCGGAGGAGATCACAGGCCAT
  CTGCACAGAGCCAAGCAGCTGGGCCTGAAGAACATAATGGCGCTGAGGGGAGACCCTGTAGGTGACCACT
  GGGAAGCAGAGGAAGGAGGCTTCAGCTATGCCACAGACCTGGTGAAGCACATCCGGACCGAGTTTGCTGA
  CTATTTTGACATCTGTGTGGCAGGTTACCCCAGAGGCCACCCCGATGCAGAGAGCTTCGAGGATGACCTG
  AAGCATTTGAAGGAGAAGGTATCTGCAGGCGCCGACTTCATTATCACTCAGCTCTTCTTTGAGGCCAGCA
  CCTTCTTCAGCTTTGTGAAGGCCTGCACAGAGATAGGCATCTCTTGCCCTATCCTGCCTGGGATCTTCCC
  TATTCAGGGCTACACTTCCCTTCGGCAGCTTGTAAAACTGTCCAAGCTGGAGGTGCCACAGAAGATCAAG
  GATGTAATTGAGCCCATCAAAGACAACGATGCTGCCATCCGCAACTACGGCATTGAGCTGGCTGTAAGCC
  TGTGCCGGGAGCTGCTGGACAGTGGCTTGGTGCCAGGCCTCCACTTCTATACCCTCAACCGCGAGGTGGC
  CACCATGGAGGTGCTAAAGCAACTGGGCATGTGGACCGAGGACCCCAGGCGTCCCTTGCCCTGGGCTCTC
  AGTGCGCATCCCAAGCGCCGGGAGGAAGATGTCCGTCCCATCTTCTGGGCCTCCAGACCAAAGAGCTACA
  TCTACCGCACACAGGACTGGGATGAGTTTCCTAACGGCCGCTGGGGTAATTCTTCCTCACCAGCCTTTGG
  GGAGCTGAAAGACTACTACCTCTTCTACCTGAAAAGCAAGTCCCCCAGGGAGGAGCTGCTGAAGATGTGG
  GGCGAGGAGCTCACCAGCGAAGAGAGTGTCTTTGAAGTCTTTGAACACTACCTCTCTGGAGAGCCGAATC
  GCCATGGCTACAGAGTAACCTGCCTGCCCTGGAACGATGAACCCCTGGCAGCGGAAACCAGCCTGATGAA
  GGAAGAGCTGCTCCGCGTGAACAGGCTGGGCATCCTCACCATCAACTCTCAGCCCAACATCAACGCAAAA
  CCATCCTCAGACCCTGTTGTGGGCTGGGGCCCCAGTGGGGGTTATGTCTTCCAGAAGGCCTACCTCGAAT
  TCTTCACCTCCCGTGAAACTGTGGAGGCGCTTCTGCAGGTGCTGAAGACATACGAGCTGCGGGTCAACTA
  CCACATCGTGGACGTGAAGGGAGAGAACATCACTAATGCCCCTGAGCTGCAGCCCAATGCCGTGACGTGG
  GGCATCTTCCCGGGTCGAGAGATCATCCAGCCTACTGTGGTGGACCCCATCAGCTTCATGTTCTGGAAGG
  ATGAGGCCTTTGCCCTGTGGATCGAGCAGTGGGGCAAGCTATACGAGGAGGAGTCGCCATCCCGCATGAT
  CATCCAATACATCCATGACAACTATTTCCTGGTCAACCTGGTGGACAACGAGTTCCCGCTGGACAGCTGC
  CTGTGGCAGGTGGTGGAGGACACGTTTGAGCTGCTCAACAGGCATCCCACGGAGAGAGAGACACAGGCTC
  CA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209788 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAE
  GAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGH
  LHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDL
  KHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIK
  DVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWAL
  SAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMW
  GEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAK
  PSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTW
  GIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSC
  LWQVVEDTFELLNRHPTERETQAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010840
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010840.1, NM_010840.2, NM_010840.3, NP_034970.2
• RefSeq Size: 6008 bp
• RefSeq ORF: 1965 bp
• Locus ID: 17769
• MW: 74.6 kDa