Aplp1 (NM_007467) Mouse Tagged ORF Clone

CAT#: MR209792

Aplp1 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aplp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209792 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCACCAGCCCCGCCGCTCGCGGTCAGGGTCGCCGCTGGCGACCGCCGCCGCTGCCGCTGTTGC
  TGCCACTGTCATTGCTGCTTCTGCGCGCGCAGCTCGCCGTCGGGAACCTGGCTGTTGGGAGCCCCAGCGC
  GGCCGAGGCTCCGGGGTCGGCTCAAGTGGCTGGACTATGTGGGCGTCTAACCCTTCACCGGGACCTGCGC
  ACCGGCCGCTGGGAACCAGACCCACAGCGATCACGACGCTGTCTTCTGGACCCGCAGCGCGTGCTGGAGT
  ACTGCAGACAGATGTACCCCGAGCTGCACATAGCACGCGTGGAGCAGGCTGCACAGGCCATCCCGATGGA
  GCGCTGGTGTGGGGGTACCCGGAGTGGCAGATGCGCCCACCCCCACCATGAGGTTGTGCCCTTCCATTGC
  CTGCCTGGCGAATTCGTGAGTGAAGCCCTGCTAGTGCCCGAAGGCTGTCGGTTCTTGCACCAGGAGCGTA
  TGGACCAGTGTGAGAGTTCAACCAGGAGGCATCAGGAGGCTCAGGAGGCCTGCAGCTCTCAGGGCCTCAT
  CCTGCATGGCTCTGGCATGCTTTTGCCCTGTGGCTCTGATCGGTTCCGAGGTGTGGAGTATGTATGCTGT
  CCACCTCCTGCAACTCCCAACCCATCTGGGATGGCAGCTGGTGACCCCTCTACCCGGTCCTGGCCCCTGG
  GGGGCAGAGCAGAGGGAGGTGAGGATGAAGAGGAGGTGGAATCTTTCCCTCAGCCAGTAGACGATTACTT
  CGTAGAGCCCCCTCAGGCTGAAGAAGAAGAGGAAGAGGAGGAAGAAAGGGCCCCACCTCCCAGCTCCCAC
  ACCCCTGTCATGGTTAGCAGAGTCACTCCCACCCCAAGGCCTACTGATGGTGTGGATGTTTACTTTGGCA
  TGCCTGGGGAAATCGGCGAGCATGAGGGTTTCCTGAGGGCCAAGATGGACCTGGAGGAGCGTAGGATGCG
  CCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCTGACAGCCAATCTAAGAACCTGCCAAAGGCGGAC
  AGGCAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACCCTGGAAGAACAAGTGTCTGGTGAACGGC
  AACGCCTGGTGGAGACCCACGCCACCAGAGTCATCGCTCTGATCAACGACCAGCGCCGAGCAGCCCTGGA
  AGGTTTCCTGGCAGCCTTACAGGGCGATCCGCCTCAGGCTGAGCGAGTTCTGATGGCCCTGAGGCGCTAC
  CTGCGCGCCGAGCAGAAAGAGCAGAGGCACACTCTGAGGCACTACCAGCACGTGGCCGCAGTGGATCCTG
  AGAAGGCCCAGCAGATGCGCTTTCAGGTCCAGACCCACCTTCAGGTGATCGAAGAGCGAATGAATCAGAG
  CCTGGGGCTGCTCGACCAGAACCCTCACCTGGCTCAGGAGCTGCGGCCACAGATCCAGGAGCTTCTCCTT
  GCTGAACACTTGGGTCCCAGTGAACTGGACGCCTCTGTGCCCGGGAGCAGCAGTGAGGACAAAGGTAGCC
  TCCAGCCTCCCGAATCCAAGGACGATCCCCCAGTGACCCTTCCAAAAGGGTCCACAGATCAAGAGTCATC
  CTCCTCTGGGAGAGAGAAGCTAACTCCACTGGAGCAGTATGAGCAAAAGGTGAATGCATCCGCCCCGAGG
  GGGTTTCCCTTCCACTCGTCAGATATCCAGCGGGATGAACTGGCTCCTTCCGGGACTGGAGTGTCCCGAG
  AGGCCTTGTCAGGTCTGCTGATCATGGGAGCTGGAGGAGGCTCCCTCATTGTCCTATCCTTGCTGCTTCT
  GCGCAAGAAGAAACCCTATGGGACTATCAGCCATGGAGTGGTGGAGGTGGACCCCATGCTGACCCTGGAG
  GAGCAGCAGCTCCGGGAACTTCAGAGGCATGGCTATGAGAACCCCACCTACCGCTTCCTGGAAGAACGAC
  CT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209792 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPTSPAARGQGRRWRPPPLPLLLPLSLLLLRAQLAVGNLAVGSPSAAEAPGSAQVAGLCGRLTLHRDLR
  TGRWEPDPQRSRRCLLDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWCGGTRSGRCAHPHHEVVPFHC
  LPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCC
  PPPATPNPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEEERAPPPSSH
  TPVMVSRVTPTPRPTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKAD
  RQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMALRRY
  LRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHLAQELRPQIQELLL
  AEHLGPSELDASVPGSSSEDKGSLQPPESKDDPPVTLPKGSTDQESSSSGREKLTPLEQYEQKVNASAPR
  GFPFHSSDIQRDELAPSGTGVSREALSGLLIMGAGGGSLIVLSLLLLRKKKPYGTISHGVVEVDPMLTLE
  EQQLRELQRHGYENPTYRFLEERP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007467
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007467.1, NM_007467.2, NM_007467.3, NP_031493.2
• RefSeq Size: 2408 bp
• RefSeq ORF: 1965 bp
• Locus ID: 11803
• MW: 72.8 kDa
• Gene Summary: May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I. [UniProtKB/Swiss-Prot Function]