Smpd3 (NM_021491) Mouse Tagged ORF Clone

CAT#: MR209795

Smpd3 (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase 3, neutral (Smpd3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smpd3
• Synonyms: 4631433G07Rik; AI427456; AW537966; fro; nSMase2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209795 representing NM_021491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTGTACACGACCCCCTTTCCTAATAGCTGTCTGTCTGCCCTCCACGCCGTGTCCTGGGCCCTCA
  TCTTCCCATGTTACTGGCTGGTGGACCGGCTGTTGGCCTCTTTCATACCCACCACCTATGAGAAGCGCCA
  GAGGGCAGATGACCCCTGCTGCCTGCAGCTGTTCTGCACGGTCCTCTTCACGCCAGTCTACCTGGCTCTG
  CTTGTGGCTGCACTGCCCTTTGCCTTTCTCGGGTTCATCTTCTGGTCTCCACTGCAGTCTGCCCGCCGGC
  CCTACTCCTACTCCCGGCTAGAAGACAAGAACCCAGCTGGTGGGGCAGCCCTGCTTAGTGAATGGAAGGG
  CACGGGGGCAGGCAAAAGCTTTTGCTTCGCCACGGCCAATGTCTGCCTCCTCCCCGACTCACTCGCGAGG
  CTCAATAATGTGTTCAACACTCAAGCTCGGGCCAAAGAGATTGGGCAGAGAATCCGCAATGGGGCCGCCA
  GGCCTCAGATCAAGATCTACATCGATTCTCCCACCAACACCTCCATCAGTGCAGCCAGCTTCAGCAGCCT
  GGTGTCGCCACAGGGTGGTGATGGATCCAGAGCTGTTCCTGGGAGCATTAAGAGGACAGCCTCTGTGGAA
  TACAAGGGGGATGGTGGGCGTCACCCCAGCGACGAGGCTGCCAACGGCCCAGCCTCTGGAGAACAGGCTG
  ACGGAAGCCTTGAGGACAGCTGCATTGTGCGAATTGGTGGCGAGGAAGGAGGTCGGCCACAAGAAGCTGA
  TGACCCTGCTGCCGGGAGCCAAGCCAGGAATGGGGCTGGTGGGACCCCAAAGGGCCAGACACCCAACCAC
  AATCAGCGAGATGGGGATTCTGGGAGCCTGGGCAGCCCCTCAGCCTCCAGGGAGTCCTTGGTGAAGGCAC
  GGGCTGGGCAAGACAGTGGTGGCAGCGGAGAGCCAGGGGCTAACAGCAAGCTGCTGTATAAGACCTCTGT
  GGTGAAGAAGGCAGCAGCCCGCAGGAGGCGCCACCCTGATGAAGCCTTTGACCACGAGGTCTCAGCCTTC
  TTCCCGGCCAATCTGGACTTCCTGTGCCTGCAGGAGGTGTTTGACAAGCGTGCGGCTGCTAAGTTGAAAG
  AGCAGCTACACGGCTACTTCGAGTACATCCTGTATGATGTTGGGGTCTACGGTTGTCATGGTTGCTGCAA
  TTTCAAATGTCTCAACAGCGGTCTCTTCTTCGCCAGCCGCTACCCTGTCATGGACGTGGCCTATCACTGT
  TACCCCAATGGGTGCAGCTTCGATGCCCTGGCCTCTAAGGGAGCTCTGTTTCTCAAGGTGCAGGTGGGAA
  GCACACCTCAGGACCAAAGAATTGTTGGGTACATCGCCTGCACACACCTGCATGCCCCACCAGAGGACAG
  TGCCGTCCGGTGTGAGCAGCTGGACCTGCTTCAGGACTGGCTGGCTGATTTCCGAAAATCTACCTCCTCG
  ACCAGCACAGCCAACCCCGAGGAGCTGGTGGTGTTTGACGTCATCTGTGGAGATTTGAACTTTGATAACT
  GCTCTTCTGATGACAAACTGGAGCAGCAGCACTCGCTCTTTACTCGCTACAAAGACCCCTGCCGCCTTGG
  GCCCGGGGAGGAGAAGCCTTGGGCCATTGGCACCCTGCTGGACACAAACGGTCTCTATGATGAGGATGTG
  TGTACCCCGGACAACCTTCAAAAGGTGTTGGAGAGCGAGGAAGGCCGCAGGGAGTATCTGGCCTTCCCCA
  CCAGCAAGAGCCCAGGTGCTGGGCAGAAGGGCCGGAAGGATCTGCTGAAGGGCAATGGCCGCCGCATCGA
  CTACATGCTGCACGCCGAGGAGGGGCTGTGCCCTGACTGGAAGGCTGAGGTAGAAGAATTCAGTTTTATC
  ACCCAGCTGTCCGGCCTGACCGACCACCTCCCCGTGGCCATGCGGCTGATGGTGTCTGCAGGGGAAGAGG
  AGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209795 representing NM_021491
  Red=Cloning site Green=Tags(s)
  
  MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLLASFIPTTYEKRQRADDPCCLQLFCTVLFTPVYLAL
  LVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKNPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLAR
  LNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGSRAVPGSIKRTASVE
  YKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRPQEADDPAAGSQARNGAGGTPKGQTPNH
  NQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGANSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAF
  FPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHC
  YPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAVRCEQLDLLQDWLADFRKSTSS
  TSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDTNGLYDEDV
  CTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEVEEFSFI
  TQLSGLTDHLPVAMRLMVSAGEEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021491
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021491.1, NM_021491.2, NM_021491.3, NP_067466.1
• RefSeq Size: 5148
• RefSeq ORF: 1968
• Locus ID: 58994
• MW: 71.6 kDa