Egfr (NM_007912) Mouse Tagged ORF Clone

CAT#: MR209803

Egfr (Myc-DDK-tagged) - Mouse epidermal growth factor receptor (Egfr), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Egfr
• Synonyms: 9030024J15Rik; AI552599; Erbb; Errb1; Errp; wa-2; wa2; Wa5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209803 representing NM_007912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACCCTCAGGGACCGCGAGAACCACACTGCTGGTGTTGCTGACCGCGCTCTGCGCCGCAGGTGGGG
  CGTTGGAGGAAAAGAAAGTCTGCCAAGGCACAAGTAACAGGCTCACCCAACTGGGCACTTTTGAAGACCA
  CTTTCTGAGCCTGCAGAGGATGTACAACAACTGTGAAGTGGTCCTTGGGAACTTGGAAATTACCTATGTG
  CAAAGGAATTACGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCCGGCTATGTCCTCATTGCCCTCA
  ACACCGTGGAGAGAATCCCTTTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTTTATGAAAACACCTA
  TGCCTTAGCCATCCTGTCCAACTATGGGACAAACAGAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA
  CAGGAAATCCTGATTGGTGCTGTGCGATTCAGCAACAACCCCATCCTCTGCAATATGGATACTATCCAGT
  GGAGGGACATCGTCCAAAACGTCTTTATGAGCAACATGTCAATGGACTTACAGAGCCATCCGAGCAGTTG
  CCCCAAATGTGATCCAAGCTGTCCCAATGGAAGCTGCTGGGGAGGAGGAGAGGAGAACTGCCAGAAATTG
  ACCAAAATCATCTGTGCCCAGCAATGTTCCCATCGCTGTCGTGGCAGGTCCCCCAGTGACTGCTGCCACA
  ACCAATGTGCTGCGGGGTGTACAGGGCCCCGAGAGAGTGACTGTCTGGTCTGCCAAAAGTTCCAAGATGA
  GGCCACATGCAAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACCTATCAGATGGATGTCAAC
  CCTGAAGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAGTGCCCCCGAAACTACGTGGTGACAGATC
  ATGGCTCATGTGTCCGAGCCTGTGGGCCTGACTACTACGAAGTGGAAGAAGATGGCATCCGCAAGTGTAA
  AAAATGTGATGGGCCCTGTCGCAAAGTTTGTAATGGCATAGGCATTGGTGAATTTAAAGACACACTCTCC
  ATAAATGCTACAAACATCAAACACTTCAAATACTGCACTGCCATCAGCGGGGACCTTCACATCCTGCCAG
  TGGCCTTTAAGGGGGATTCTTTCACGCGCACTCCTCCTCTAGACCCACGAGAACTAGAAATTCTAAAAAC
  CGTAAAGGAAATAACAGGCTTTTTGCTGATTCAGGCTTGGCCTGATAACTGGACTGACCTCCATGCTTTC
  GAGAACCTAGAAATAATACGTGGCAGAACAAAGCAACATGGTCAGTTTTCTTTGGCGGTCGTTGGCCTGA
  ACATCACATCACTGGGGCTGCGTTCCCTCAAGGAGATCAGTGATGGGGATGTGATCATTTCTGGAAACCG
  AAATTTGTGCTACGCAAACACAATAAACTGGAAAAAACTCTTCGGGACACCCAATCAGAAAACCAAAATC
  ATGAACAACAGAGCTGAGAAAGACTGCAAGGCCGTGAACCACGTCTGCAATCCTTTATGCTCCTCGGAAG
  GCTGCTGGGGCCCTGAGCCCAGGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGA
  GAAATGCAACATCCTGGAGGGGGAACCAAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA
  GAATGTCTGCCCCAGGCCATGAACATCACCTGTACAGGCAGGGGACCAGACAACTGCATCCAGTGTGCCC
  ACTACATTGATGGCCCACACTGTGTCAAGACCTGCCCAGCTGGCATCATGGGAGAGAACAACACTCTGGT
  CTGGAAGTATGCAGATGCCAATAATGTCTGCCACCTATGCCACGCCAACTGTACCTATGGATGTGCTGGG
  CCAGGTCTTCAAGGATGTGAAGTGTGGCCATCTGGGTACGTTCAATGGCAGTGGATCTTAAAGACCTTTT
  GGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209803 representing NM_007912
  Red=Cloning site Green=Tags(s)
  
  MRPSGTARTTLLVLLTALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYV
  QRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNL
  QEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKL
  TKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVN
  PEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLS
  INATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAF
  ENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKI
  MNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHP
  ECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAG
  PGLQGCEVWPSGYVQWQWILKTFWI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007912
• ORF Size: 1965 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007912.1, NM_007912.2, NM_007912.3, NM_007912.4, NP_031938.1
• RefSeq Size: 2678 bp
• RefSeq ORF: 1968 bp
• Locus ID: 13649
• MW: 73.4 kDa