Hal (NM_010401) Mouse Tagged ORF Clone

CAT#: MR209814

Hal (Myc-DDK-tagged) - Mouse histidine ammonia lyase (Hal)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hal
• Synonyms: his; histidase; Hsd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209814 representing NM_010401
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAGGTACACAGTGCACGTGCGTGGGGAATGGCTGGCGGTGCCCTGTCAGGATGGGAAGCTCACTG
  TTGGCTGGCTGGGCCGTGAGGCTGTGCGACGCTACATGAAGAACAAACCGGACAATGGTGGCTTCACTTC
  GGTGGATGAAGTGCAATTCCTTGTGCATCGGTGCAAGGGCCTGGGCCTGCTGGACAATGAGGACGAGCTG
  GAGGTGGCCTTAGAGGACAATGAGTTCGTAGAAGTGGTGATTGAAGGTGATGTGATGTCTCCTGATTTCA
  TTCCGTCGCAGCCAGAAGGAGTTTTCCTATACAGCAAATACCGGGAGCCTGAAAAATACATTGCCCTAGA
  TGGGGACAGTCTGAGCACAGAGGATCTGGTCAACCTGGGCAAGGGACGTTACAAGATAAAGCTCACTTCA
  ATTGCTGAGAAGAAAGTGCAGCAGTCCCGGGAGGTCATCGACAGCATCATCAAAGAAAGAACAGTTGTTT
  ATGGCATCACCACAGGTTTTGGAAAATTTGCTAGAACTGTAATCCCTGCCAATAAGCTACAGGAGCTTCA
  AGTCAACTTAGTCCGTTCCCATTCTTCAGGTGTTGGGAAACCACTCAGTCCTGAGAGGTGTCGGATGCTC
  TTGGCTTTGAGGATCAACGTCTTAGCCAAAGGTTACAGTGGGATTTCCTTGGAGACGCTGAAACAAGTTA
  TTGAAGCATTTAATGCCTCCTGCCTGTCCTATGTTCCAGAGAAAGGAACTGTGGGTGCCAGCGGAGACCT
  TGCCCCACTCTCTCATCTTGCTCTGGGGCTCATCGGAGAAGGAAAGATGTGGTCCCCGAAGAGTGGCTGG
  GCAGATGCTAAATATGTTCTAGAAGCCCATGGACTGAAACCAATTGTCTTGAAACCAAAAGAAGGCCTGG
  CACTCATCAATGGGACACAGATGATCACTTCCCTGGGCTGTGAAGCCCTGGAGCGAGCCAGCGCCATTGC
  CCGGCAAGCTGATATTGTGGCTGCCTTGACCCTGGAGGTGCTGAAGGGCACCACCAAAGCCTTCGATACC
  GACATCCATGCTGTCCGACCTCACCGTGGGCAAATTGAAGTTGCTTTCCGATTCCGGTCCCTCCTGGACT
  CGGATCACCACCCATCAGAAATCGCAGAAAGCCACAGGTTCTGCGATCGTGTTCAGGACGCGTACACCTT
  ACGCTGCTGTCCACAGGTCCACGGTGTGGTGAACGACACAATAGCCTTTGTGAAGGACATCATCACTACA
  GAACTGAACAGCGCCACAGACAATCCTATGGTCTTTGCCAGTCGAGGGGAGACAATTTCAGGAGGAAACT
  TCCATGGTGAATACCCAGCCAAAGCCCTGGACTATTTGGCCATTGGTGTCCATGAACTTGCGGCAATTAG
  TGAAAGAAGAATCGAAAGGCTGTGTAATCCTTCCCTCAGCGAGCTGCCTGCCTTCCTGGTGGCTGAAGGA
  GGTCTCAATTCTGGGTTCATGATAGCCCACTGCACCGCAGCAGCCCTGGTGTCTGAGAGCAAGGCTCTGT
  GCCACCCTTCATCTGTGGACTCACTCTCGACCAGTGCTGCTACAGAAGACCACGTCTCCATGGGAGGATG
  GGCAGCCAGGAAAGCCCTCAGGGTCGTCGAGCACGTGGAACAAGTGCTAGCCATTGAGCTTCTCGCGGCC
  TGCCAGGGTATAGAGTTTCTGCGCCCCCTGAAAACAACCACTCCGCTGGAGAAGGTGTACGACCTGGTGC
  GCTCCGTAGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCGGACATTGAGGCAGCACACAGGTTGCT
  TCTTGACCAAAAGGTTTGGGAGGTTGCCGCACCGTACATCGAGAAATACAGAATGGAGCACATTCCGGAA
  TCCAGACCGCTTTCTCCAACAGCGTTTTCACTAGAATCTCTCCGAAAGAACTCAGCCACAATCCCAGAGT
  CTGACGATCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209814 representing NM_010401
  Red=Cloning site Green=Tags(s)
  
  MPRYTVHVRGEWLAVPCQDGKLTVGWLGREAVRRYMKNKPDNGGFTSVDEVQFLVHRCKGLGLLDNEDEL
  EVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGRYKIKLTS
  IAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRML
  LALRINVLAKGYSGISLETLKQVIEAFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGW
  ADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEALERASAIARQADIVAALTLEVLKGTTKAFDT
  DIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITT
  ELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEG
  GLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVVEHVEQVLAIELLAA
  CQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPE
  SRPLSPTAFSLESLRKNSATIPESDDL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010401
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010401.1, NM_010401.2, NM_010401.3, NM_010401.4, NP_034531.1
• RefSeq Size: 2758
• RefSeq ORF: 1974
• Locus ID: 15109
• MW: 72.3 kDa
• Gene Summary: This gene encodes a member of the histidase protein family. The encoded protein is a cytosolic enzyme which catalyzes the first reaction in histidine catabolism. Defects in this protein cause histidinemia, which is characterized by increased histidine in blood, urine, and cerebrospinal fluid. [provided by RefSeq, Dec 2015]