D14Ertd231e (BC028953) Mouse Tagged ORF Clone

CAT#: MR209828

D14Ertd231e (Myc-DDK-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:38896 IMAGE:5362016)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D14Ertd231e
• Synonyms: BC028953|D14Ertd231e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209828 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTGTATTGCCTGTCAGGGCACCCAACTTTACCATGCAACGTACTCAAATTCAAATCCACTACTA
  TTATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTCTTGTTCAAAGCCC
  CAGGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAAGGAGCTAAAAGAG
  TGCTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAGCTAATAGACCTGT
  CTACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACATCACCGAGCACAC
  AGGATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCTCATGGAAGAGCTG
  GTGAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAGGATATACAGCGGC
  TGCTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACACGATGCAGGAGGT
  GAACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTTTGGTGCTGTCCAG
  GTGACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCACATTATGAGAAAG
  TATCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAGCTTCTCTCAAAAA
  CAGTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGGCATGGTGGGCGAG
  TTCTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGCTATCCTTCAGGCG
  TGATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCAACATCCCTTTCTA
  CTTCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTGGCTTTGTCACAAC
  AAACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAGACGAATAAGCTGA
  AGCACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGCTGTTCACCGGACA
  TCCTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAGCCTCAACACCATC
  ATATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTGGCCATGAAGTGCA
  TCTACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTACAGCCCCT
  GCACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGATGGACCTCATGATC
  GACTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTCAAACGACGCTATG
  AGAAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGCCTGGCATCTCCTT
  GGCGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCCTCCCAAGCCCACC
  CAGCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAAGTACTGAAGCCTC
  TGCTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCTTCAGTGACATTAA
  GGTGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCATCCAGATTGAAGAA
  GACTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGACCTTGTCCTCAGGT
  TCCTGCAGAAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209828 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSTLNFLPLPLVQSPRLSNLPGWSLKDGNAFLDKELKE
  CSGHVFVDSVPEFCLPETELIDLSTVDVILISNYHCMMALPYITEHTGFTGTVYATEPTMQIGRLLMEEL
  VNFIERVPKAQSASLWKNKDIQRLLPSPLKDAVEVSTWRRCYTMQEVNSALSKIQLVGYSQKIELFGAVQ
  VTPLSSGYALGSSNWIIQSHYEKVSYVSGSSLLTTHPQPMDQASLKNSDVLILTGLTQIPTANPDGMVGE
  FCSNLALTVRNGGNVLVPCYPSGVIYDLLECLYQYIDSAGLSNIPFYFISPVANSSLEFSQIFAEWLCHN
  KQSKVYLPEPPFPHAELIQTNKLKHYRSIHGDFSNDFRQPCVLFTGHPSLRFGDVVHFMELWGKSSLNTI
  IFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPAQAHRMDLMI
  DCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATVSAVLHTKDNKHVLQPPPKPT
  QPTSSKKRKRVNEDIPDCKVLKPLLSGSIPVEQFVQTLEKHGFSDIKVEDTAKGHIVLLQEAETLIQIEE
  DSTHIICDNDETLRVRLRDLVLRFLQKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC028953
• ORF Size: 1974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028953.1
• RefSeq Size: 2212 bp
• RefSeq ORF: 1976 bp
• Locus ID: 210925
• MW: 74.1 kDa