Angel1 (NM_144524) Mouse Tagged ORF Clone

CAT#: MR209894

Angel1 (Myc-DDK-tagged) - Mouse angel homolog 1 (Drosophila) (Angel1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angel1
• Synonyms: 1110030H02Rik; mKIAA0759
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209894 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGCGTCGTGCCTGTATTACCTGCTGCTGCCAGCCGCGCGCCTCTTCCGCTTTCTCTCAGATGCTT
  TCTTTACATGTCGAAAAAATGCCCTTCTGGCGAAGAGCTCATCTCCCCAGGTAGAGGGTAACTTTGCCAT
  GGCCCCTCGGGGGCCTGACCAAGAAGAGTGTGAGGGCCTGCTGCAGCAGTGGCGAGAAGAAGGGTGGAAC
  CAGACACCCTCAACTGCAAGCGAGGGTCCCCTTGCGGATAAGGGACTAGCTGAGAGCAGTCTGGCCCTCC
  TGATGGATAATTCTGGAGAACAGGATGCTGCCTCGGAGGACAAGTGGTCCAGCAGGCAGCTGAGTGACCT
  TCGGGCAGCAGAGAACCTGGACCAGCCTTTCCCTGAGGTGCTAGGGGAGGAGCCTCTGGCCGAGGTTGAG
  GGTCCTTTGTGGGCGGCTGTTCCGGTACAGACAGGGCCCCAGTATGCAGATTGTGCTGTTCTTCCAATGG
  GTGCGATGGCCGCAGAACAGTGGGAAGAGGACCCTGCAATGGTGGCCTGGAGCATAGCACCGGAGCCTAT
  GCCCCAGGAGGAGACTTCCATGTGGCCCTTTGAAGGCCTGGAACAGCTGCAGCCTCCCCCAATGGAAATA
  CCTTATCATGAAATCCTGTGGCGAGAATGGGAGGATTTCTCCACACAGCCAGATGCTCAGGGTCTGGAGG
  CAGGAGACGGCCCTCAGTTCCAGTTCACTCTGATGTCGTATAATATCCTGGCCCAGGACCTGATGCAGCA
  GAGCTCAGAGCTCTATCTGCATTGCCATCCAGACATCCTCAACTGGAATTACCGCTTTGCCAACCTCATG
  CAGGAATTCCAGCACTGGGACCCGGATATCCTGTGTCTTCAGGAAGTCCAGGAAGACCACTACTGGGAGC
  AGCTGGAGCCCTCTCTGCGGATGATGGGCTTCACCTGTTTTTACAAGAGGAGGACTGGGTGTAAAACGGA
  TGGCTGTGCTGTGTGCTACAAGCCCACCAGGTTCCGTCTGCTCTGTGCCAGCCCTGTGGAGTACTTCCGG
  CCTGGGTTGGAGCTCCTCAACCGGGACAATGTGGGCTTAGTGTTGCTGCTGCAGCCGCTAGTGCCAGAAG
  GTCTGGGGCAAGTCTCAGTGGCTCCCCTGTGTGTGGCAAATACCCATGTCCTGTACAACCCGCGTCGGGG
  TGATGTCAAGCTGGCCCAGATGGCCATTCTTCTGGCCGAGGTGGACAAGGTGGCCAGGTTGTCAGATGGC
  AGCCACTGCCCCATCATCTTGTGTGGGGACCTGAATTCTGTCCCTGACTCACCTCTCTACAACTTCATCA
  GGGATGGAGAGCTCCAGTACAATGGCATGCCAGCCTGGAAGGTTTCTGGACAGGAAGACTTCTCTCATCA
  GCTTTACCAGAGGAAGCTGCAGGCCCCACTGTGGCCCAGCTCCCTGGGCATCACTGACTGCTGTCAATAT
  GTCACCTCCTGTCATCCCAAGAGATCAGAGAGACTGAAATATGGCCGAGACTTCCTACTACGATTCCGCT
  TCTGTGACCTCGCTTGCCAGCGACCAGTGGGACTGGTTCTCATGGAAGGAGTGACAGACACTAAGCCAGA
  TCGACCTGCTGGTTGGGCCGAGTGTATCTTTGAGGAAGAGATATCTGAACTTGAGCCTGTCTTCCCCAGG
  ACTATAGGCACCATCCAGCACTGCCTCCACCTGACCTCAGTGTATACCCACTTCCTGCCCCAGCATGGCT
  GCCCAGAGGTCACCACGATGCCCTTAGGGCTTGGGATGACAGTGGATTACATTTTCTTCTCAGCTGAATC
  CTGTGAGAATGAGAACAGAACCGATCACAGACTGGATCGAGATGGAACTCTCAAGCTCCTGGGCCGCCTC
  TCCCTCCTCTCTGAAGAGATTCTCTGGGCTGCCAATGGCTTACCCAACCCCTTCTACTCTTCAGACCATC
  TCTGCTTGCTTGCCAGCTTCGGCATGGAAGTCACTGCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209894 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIASCLYYLLLPAARLFRFLSDAFFTCRKNALLAKSSSPQVEGNFAMAPRGPDQEECEGLLQQWREEGWN
  QTPSTASEGPLADKGLAESSLALLMDNSGEQDAASEDKWSSRQLSDLRAAENLDQPFPEVLGEEPLAEVE
  GPLWAAVPVQTGPQYADCAVLPMGAMAAEQWEEDPAMVAWSIAPEPMPQEETSMWPFEGLEQLQPPPMEI
  PYHEILWREWEDFSTQPDAQGLEAGDGPQFQFTLMSYNILAQDLMQQSSELYLHCHPDILNWNYRFANLM
  QEFQHWDPDILCLQEVQEDHYWEQLEPSLRMMGFTCFYKRRTGCKTDGCAVCYKPTRFRLLCASPVEYFR
  PGLELLNRDNVGLVLLLQPLVPEGLGQVSVAPLCVANTHVLYNPRRGDVKLAQMAILLAEVDKVARLSDG
  SHCPIILCGDLNSVPDSPLYNFIRDGELQYNGMPAWKVSGQEDFSHQLYQRKLQAPLWPSSLGITDCCQY
  VTSCHPKRSERLKYGRDFLLRFRFCDLACQRPVGLVLMEGVTDTKPDRPAGWAECIFEEEISELEPVFPR
  TIGTIQHCLHLTSVYTHFLPQHGCPEVTTMPLGLGMTVDYIFFSAESCENENRTDHRLDRDGTLKLLGRL
  SLLSEEILWAANGLPNPFYSSDHLCLLASFGMEVTAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144524
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144524.1, NM_144524.2, NP_653107.1
• RefSeq Size: 3867 bp
• RefSeq ORF: 2004 bp
• Locus ID: 68737
• MW: 75.3 kDa