Fam189b (NM_001014995) Mouse Tagged ORF Clone

CAT#: MR209910

Fam189b (Myc-DDK-tagged) - Mouse family with sequence similarity 189, member B (Fam189b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fam189b
• Synonyms: 1110013L07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209910 representing NM_001014995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCCTCGCCCAGTGACTCCAGTCGCTCGCTGACCAGCCGGCCCAGTACCCGGGGCCTTACCCACC
  TCCGCCTCCACCGACCCTGGCTCCAGGCCCTGCTCACGTTGGGGCTGGCCCAGGTGCTCTTGGGCATCCT
  GGTGATAACCTTCAGCATGGTGGCCTCCTCTGTCACCACCACCGAGAGCATCAAGAGGTCCTGCCCATCT
  TGGGCTGGGTTCTCGCTGGCGTTCTCCGGGTTGGTTGGCATTGTGTCCTGGAAGCGGCCATTCACTCTAG
  TGATCTCTTTCTTCTCCTTGCTTTCGGTGCTCTGTGTCATGCTTAGCATGGCTGGCTCTGTTCTCTCCTG
  TAAGAATGCTCAGCTGGCTCGGGACTTCCGAGAGTGCTCCATGGAAGGGAAGGTCTGTGTATGCTGCCCA
  CCTATCCCCCTACACAGACCTTGCCCAGAATGGGGCCAGGAGCTGAAAGTTGCCCTCAACTCCACCTGTG
  ATGAAGCCAGAGGGGCCCTCAAGAACCTGCTCTTCAGTGTCTGTGGCCTTACCATTTGTGCTGCAATAAT
  CTGTACCCTCTCTGCTATTGTCTGCTGTGTCCAGATCTTCTCCCTGGATCTGGTGCATATGCAGCTTGCC
  CCTGAACGGTCAGTCTCAGGCCCCCTAGGGCCCCTGGCGTGTACATCCTCGCCTCCAGCTCCTCTCCTAC
  ACACCATGCTGGACTTGGAGGAATTTGTCCCGCCTGTGCCCCCACCACCCTACTACCCTCCAGAGTACAC
  CTGCAGTTCAGAGACAGATGCACAAAGCATCACGTATAATGGCTCCATGGATAGCCCAGTGCCCTTGTAT
  CCTACTGACTGCCCCCCTTCTTACGAGGCCGTCATGGGGCTGCGAAGAGACAGCCAGGCCACTCTGTTTG
  ACCCTCAGCTTCACGATGGCTCCTGTGTCTGTGAGCGGGTGGCCTCCATTGTGGACGTGTCCATGGACAG
  TGGGTCCCTGGTGTTGTCTGCCATTGGCGACCTCCCCGGGGGCTCTAGCCCATCAGAAGACTCATGCCTG
  CTGGAACTCCAGGGCTCTGTGCGCTCCGTCGATTATGTGCTCTTCCGCTCCATCCAGCGCAGCCGCGCAG
  GCTACTGCCTTAGCCTGGACTGCGGCCTCCGGGGTCCCTTTGAAGACAGTCCCTTGCCAAGGCGACCCCC
  ACGGGCTGCCCGCTCCTATTCCTGCTCAGCCCCTGAAGCCCCACCCCCACTGGGTGCCCCCACAGCTGCT
  CGCAGTTGCCACCGGTTAGAAGGCTGGCCGCCCTGGGTGGGACCTTGTTTCCCCGAGCTGAGGCGGCGGG
  TCCCCCGAGGAGGCAGCCGCTCAGCTGCACCCCCTCCTGCCCGAGCACCAGCTCGACGCTTCAGTGATAG
  CTCAGGTTCCCTCACCCCACCCGGACACCGACCTCCTCACCGGACACCTCCTCCACCACTGCTGCTGCCC
  AGGTCTCACAGCGACCCTGGCATTACCACCTCCAGCGACATCGCTGACTTCAGGGACCTTTATACCAAAG
  TGCTTGAGGAAGAAGCTGCCTCTGTCTCCTCTGCAGACACAGGGCTCTGCTCTGAAGCCTGCCTTTTCCG
  TCTAGCCCGCTGCCCTTCCCCCAAGTTGCTACGTGCTCGCTCAGCCGAGAAACGCCGCCCAGTGCCCACC
  TTCCAGAAGGTTCCCCTGCCTTCAGGTCCTACACCTGCCCACTCTCTAGGGGACCTGAAAGGAAGCTGGC
  CAGGCCGGGGCCTAGTCACTCGTTTCCTCCAGCTGTCTAGGAGGTCTCCAGACCCTACAGGAACTGGAGC
  CCATGGATATAAGCAAGTGCGCAGGAGCCCATGGGGGCGACCAGGTCGAGAGAGCCTCCACCTTCGCAGC
  TGTGGTGATTTGAGCTCTGGCTCCTCCCTAAGGCGACTCTTGTCTGCCCGCAGGCTAGAGCATGGCATCC
  GCCCACACAGCCTCAGCCTCAATGGAGGCAGCCGGGAGACTGGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209910 representing NM_001014995
  Red=Cloning site Green=Tags(s)
  
  MMPSPSDSSRSLTSRPSTRGLTHLRLHRPWLQALLTLGLAQVLLGILVITFSMVASSVTTTESIKRSCPS
  WAGFSLAFSGLVGIVSWKRPFTLVISFFSLLSVLCVMLSMAGSVLSCKNAQLARDFRECSMEGKVCVCCP
  PIPLHRPCPEWGQELKVALNSTCDEARGALKNLLFSVCGLTICAAIICTLSAIVCCVQIFSLDLVHMQLA
  PERSVSGPLGPLACTSSPPAPLLHTMLDLEEFVPPVPPPPYYPPEYTCSSETDAQSITYNGSMDSPVPLY
  PTDCPPSYEAVMGLRRDSQATLFDPQLHDGSCVCERVASIVDVSMDSGSLVLSAIGDLPGGSSPSEDSCL
  LELQGSVRSVDYVLFRSIQRSRAGYCLSLDCGLRGPFEDSPLPRRPPRAARSYSCSAPEAPPPLGAPTAA
  RSCHRLEGWPPWVGPCFPELRRRVPRGGSRSAAPPPARAPARRFSDSSGSLTPPGHRPPHRTPPPPLLLP
  RSHSDPGITTSSDIADFRDLYTKVLEEEAASVSSADTGLCSEACLFRLARCPSPKLLRARSAEKRRPVPT
  FQKVPLPSGPTPAHSLGDLKGSWPGRGLVTRFLQLSRRSPDPTGTGAHGYKQVRRSPWGRPGRESLHLRS
  CGDLSSGSSLRRLLSARRLEHGIRPHSLSLNGGSRETGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014995
• ORF Size: 2007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014995.1, NP_001014995.1
• RefSeq Size: 3077 bp
• RefSeq ORF: 2010 bp
• Locus ID: 68521
• Cytogenetics: 3 F1
• MW: 72.5 kDa