Wdr48 (NM_026236) Mouse Tagged ORF Clone

CAT#: MR209959

Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (Wdr48)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr48
• Synonyms: 8430408H12Rik; mKIAA1449; Uaf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209959 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCATCACCGGCAGAACACAGCCGGGCGGAGGAAAGTGCAGGTTTCTTATGTAATTCGAGATG
  AAGTGGAGAAATACAATCGGAATGGAGTCAATGCCCTGCAACTGGACCCAGCACTCAACAGACTCTTCAC
  AGCAGGTCGAGACTCCATCATAAGAATATGGAGTGTCAATCAGCACAAGCAAGATCCGTACATAGCATCT
  ATGGAGCACCATACAGACTGGGTAAATGATGTCGTGCTCTGTTGCAATGGGAAAACGCTAATTTCTGCTT
  CTTCAGACACAACAGTCAAAGTCTGGAATGCACATAAAGGGTTCTGCATGTCCACACTCAGGACGCATAA
  GGACTATGTAAAGGCCTTAGCGTACGCCAAGGATAAAGAGCTGGTAGCGTCAGCTGGGCTGGACAGACAG
  ATATTCCTTTGGGATGTGAACACTCTGACGGCACTGACTGCCTCAAACAACACCGTTACAACTTCTTCTT
  TAAGTGGGAACAAAGACTCCATTTATAGCCTGGCCATGAACCAACTGGGAACAATCATTGTATCAGGGTC
  CACTGAGAAGGTTCTGAGAGTGTGGGATCCAAGAACCTGCGCGAAACTCATGAAGCTGAAAGGACATACG
  GACAATGTGAAGGCCTTGCTGCTGCACAGGGACGGCACACAGTGCCTCTCTGGGAGTTCTGACGGGACCA
  TTCGCCTGTGGTCCCTTGGCCAGCAGAGATGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGC
  CCTACAGGTCAACGATGCCTTTACACATGTATATTCTGGAGGACGAGACAGGAAAATCTACTGCACAGAC
  CTAAGGAACCCTGACATTCGGGTACTGATTTGTGAAGAAAAAGCACCAGTTCTCAAGATGGAACTTGACA
  GATCAGCAGATCCCCCTCCTGCAATCTGGGTTGCAACAACAAAGTCCACAGTAAATAAGTGGACACTGAA
  GGGAATTCATAATTTTCGAGCCTCTGGTGATTATGACAATGATTGTACAAACCCCATAACGCCCCTCTGC
  ACACAGCCCGACCAAGTTATTAAAGGGGGCGCTAGTATTATTCAGTGCCACATCCTAAATGACAAGCGAC
  ACATATTAACCAAAGATACGAATAACAATGTGGCGTACTGGGATGTATTGAAGGCATGCAAAGTGGAAGA
  TTTGGGCAAAGTGGACTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGTGTATGTTCCAAATTGGTTC
  TCAGTAGACCTAAAAACAGGGATGCTGACCATTACCCTGGATGAGAGTGACTGCTTCGCTGCCTGGGTTT
  CTGCGAAAGATGCTGGCTTTAGCAGTCCTGATGGGTCAGACCCAAAACTGAACTTAGGAGGACTTCTACT
  ACAGGCACTGCTGGAATACTGGCCCAGGACACATGTGACTCCCATGGATGAAGAAGAGAATGAAGTGAAC
  CATGTGAGTGGGGGACAGGAGAGCAGAGTCCAGAAGGGAAATGGTTATTTCCAAGTGCCCCCACACACCC
  CGGTGATCTTTGGAGAAGCTGGAGGTCGCACACTATTCAGGCTGCTCTGCCGGGACTCCGGGGGTGAGAC
  TGAGGCGATGCTTCTCAATGAGACGGTGCCACAATGGGTGATTGACATCACTGTGGATAAAAATATGCCC
  AAATTCAACAAAATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACTTTAAAAAAAGACA
  GGCTGTCTGCCAGTGACATGCTGCAGGTCCGGAAGGTGATGGAGCACGTCTACGAGAAGATTATCAACCT
  GGACAATGAGTCCCAAACCACCAGCTCTTCTAATAATGAAAAGCCAGAGCAGGAGAAGGAGGAGGACATC
  GCGGTATTGGCAGAGGAGAAAATTGAACTTCTGTGCCAGGACCAGGTTTTGGACCCAAATATGGACCTTC
  GGACAGTAAAGCACTTCATATGGAAGAGTGGTGGGGACCTCACCCTCCATTACCGTCAGAAGTCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209959 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIAS
  MEHHTDWVNDVVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQ
  IFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHT
  DNVKALLLHRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTD
  LRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLC
  TQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWF
  SVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVTPMDEEENEVN
  HVSGGQESRVQKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETEAMLLNETVPQWVIDITVDKNMP
  KFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPEQEKEEDI
  AVLAEEKIELLCQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026236
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026236.1, NM_026236.2, NM_026236.3, NP_080512.1
• RefSeq Size: 3821 bp
• RefSeq ORF: 2031 bp
• Locus ID: 67561
• MW: 76 kDa