Srpk2 (NM_009274) Mouse Tagged ORF Clone

CAT#: MR210006

Srpk2 (Myc-DDK-tagged) - Mouse serine/arginine-rich protein specific kinase 2 (Srpk2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srpk2
• Synonyms: AW226533; AW492537; AW547358; mSRPK2; Wbp6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210006 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCTCCTTTAGTTC
  CTCCTCCTCCACCACCACCACCGCCACCACCACTGCCAGACCCCGCACCCCCAGAGCCAGAGGAGGAGAT
  TCTGGGGTCAGATGATGAGGAGCAGGAGGACCCCGCAGATTACTGCAAAGGTGGCTATCATCCAGTGAAA
  ATTGGAGATCTCTTCAATGGTCGATATCATGTCATTAGAAAGCTAGGATGGGGGCACTTTTCTACTGTAT
  GGCTGTGCTGGGATATGCAAGGGAAAAGATTTGTTGCAATGAAAGTTGTAAAAAGTGCCCAGCATTATAC
  AGAGACAGCCTTGGATGAAATTAAACTACTCAAATGCGTTCGAGAAAGTGACCCCAGTGACCCAAACAAA
  GACATGGTAGTTCAGCTAATTGATGACTTCAAGATCTCAGGCATGAATGGGATACATGTCTGCATGGTCT
  TTGAAGTACTTGGTCACCATCTCCTCAAATGGATCATCAAATCCAACTATCAAGGCCTCCCAGTACGTTG
  TGTGAAGAGTATCATTCGACAGGTCCTTCAAGGGTTAGATTATCTACACAGTAAGTGCAAGATAATTCAC
  ACCGACATAAAGCCGGAAAACATCTTGATGTGTGTGGATGACGCTTACGTGAGAAGAATGGCAGCCGAAG
  CCACGGAGTGGCAGAAAGCAGGTGCTCCTCCTCCCTCTGGGTCTGCAGTGAGTACGGCTCCACAGCAAAA
  ACCTATAGGAAAAATATCTAAAAACAAAAAGAAAAAGCTGAAAAAGAAACAGAAGAGACAGGCTGAGTTG
  CTGGAGAAACGCCTACAGGAGATTGAGGAATTGGAGCGAGAAGCCGAAAGGAAAATCCTAGAGGAGAACA
  TCACCTCTGCAGAAGCTTCCGGGGAGCAGCAGGATGGAGAGTACCAGCCGGAGGTGACACTGAAAGCAGC
  CGACTTAGAGGACACAACTGAGGAAGAGACAGCAAAGGATAATGGTGAAGTTGAAGACCAGGAAGAGAAA
  GAAGATGCAGAGAAGGAGAACGCGGAGAAGGATGAAGATGATGTTGAACAGGAACTTGCAAACTTAGACC
  CTACCTGGGTGGAGTCCCCGAAAGCCAATGGCCATATTGAAAATGGCCCGTTCTCACTGGAGCAGCAGCT
  GGAGGATGAAGAGGACGATGAAGATGACTGTGCAAATCCCGAGGAGTATAACCTCGATGAGCCAAATGCA
  GAGAGTGATTACACGTATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAATGGACAACATAAGA
  CTTCAGAGTTTCCCACACCGTTGTTTTCTGGGCCCTTAGAACCTGTGGCCTGTGGCTCTGTGATTTCAGA
  GGGATCGCCACTTACCGAGCAGGAGGAAAGCAGTCCCTCCCATGACAGAAGCAGGACAGTTTCAGCCTCT
  AGTACTGGAGATTTGCCAAAAACAAAAACCCGGGCGGCTGACCTGTTGGTGAACCCTCTGGATCCACGGA
  ATGCAGATAAAATTAGAGTAAAAATTGCTGACCTGGGAAATGCTTGTTGGGTGCATAAACATTTCACAGA
  GGATATCCAGACACGTCAGTATAGGTCCATAGAGGTTTTAATAGGAGCAGGCTACAGCACACCTGCAGAC
  ATTTGGAGTACAGCTTGCATGGCATTTGAGCTCGCCACAGGAGACTATTTGTTCGAACCGCATTCTGGGG
  AAGACTATTCCAGAGATGAAGACCACATAGCCCACATCATAGAGCTGCTAGGCAGTATCCCAAGGCACTT
  TGCTCTGTCTGGAAAATATTCTCGGGAATTCTTCAATCGCAGAGGAGAACTGCGGCACATCACCAAGCTG
  AAGCCCTGGAGCCTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAATTTA
  CAGATTTCCTGATCCCAATGTTAGAGATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGCCTTCGACA
  TCCTTGGTTGAATTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210006 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPLPDPAPPEPEEEILGSDDEEQEDPADYCKGGYHPVK
  IGDLFNGRYHVIRKLGWGHFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNK
  DMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCVKSIIRQVLQGLDYLHSKCKIIH
  TDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAEL
  LEKRLQEIEELEREAERKILEENITSAEASGEQQDGEYQPEVTLKAADLEDTTEEETAKDNGEVEDQEEK
  EDAEKENAEKDEDDVEQELANLDPTWVESPKANGHIENGPFSLEQQLEDEEDDEDDCANPEEYNLDEPNA
  ESDYTYSSSYEQFNGELPNGQHKTSEFPTPLFSGPLEPVACGSVISEGSPLTEQEESSPSHDRSRTVSAS
  STGDLPKTKTRAADLLVNPLDPRNADKIRVKIADLGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPAD
  IWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKL
  KPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009274
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009274.1, NM_009274.2, NP_033300.2
• RefSeq Size: 6436 bp
• RefSeq ORF: 2049 bp
• Locus ID: 20817
• MW: 76.9 kDa